Albert Fischli

Cob(I)alamin als Katalysator. 1. Mitteilung. Reduktion von gesättigten Nitrilen in wässeriger Lösung

Co(I)alamin as Catalyst. 1. Communication. Reduction of Saturated Nitriles in Aqueous Solution Using cob(I)alamin as homogeneous catalyst in the presence of aqueous acetic acid saturated nitriles 5a–m are reduced to the corresponding aldehydes 6a–m in good yields. A possible reaction mechanism for this reduction is discussed.

Gastric (H+ + K+)-ATPase: modulation of the inhibitory properties of the novel potent antisecretagogue Ro 18-5364 by sulfhydryl reagents and nucleotides

The sulfoxide Ro 18‐5364, a potential metabolite of the IND Ro 18‐5362, is a powerful inhibitor of gastric mucosal (H+ + K+)‐ATPase, decreasing enzymatic activity with an apparent K i, of 0.1 μM. Exposure of Ro 18‐5364‐treated gastric membranes to dithiothreitol fully restored (H+ + K+)‐ATPase activity. ATP protected the enzyme against Ro 18‐5364‐induced inactivation of enzymatic activity. In addition, Ro 18‐5364 inhibited vesicular proton uptake. In proton translocation experiments reduced lipoic acid methyl ester partially restored transport properties. Dithiothreitol and mercaptoethanol were without effect. The results are discussed with respect to the possible location of essential sulfhydryl groups for enzyme activity and proton transport.