Albert J. Chulia

Characterization of ursolic acid as a lipoxygenase and cyclooxygenase inhibitor using macrophages, platelets and differentiated HL60 leukemic cells.

A new property of ursolic acid, lipoxygenase and cyclooxygenase inhibition, has been described in an acetone‐extract of heather flowers (Calluna vulgaris) wllich could help explain the anti‐inflammatory characteristics of this plant. In mouse peritoneal macrophages, human platelets and differentiated HL60 leukemic cells, ursolic acid, at 1 μM, blocks arachidonate metabolism.

Inhibition of lipoxygenase activity and HL60 leukemic cell proliferation by ursolic acid isolated from heather flowers (Calluna vulgaris)

A compound was isolated and purified from heather flowers (Calluna vulgaris) based on its ability to inhibit lipoxygenase activity. This molecule was characterized as ursolic acid by GC-MS. Ursolic acid was found to be an inhibitor of both potato tuber 5-lipoxygenase and soybean 15-lipoxygenase with IC50 values of 0.3 mM. Ursolic acid also inhibits lipoxygenase activity in mouse peritoneal macrophages at 1 microM and HL60 leukemic cells growth (IC50 = 0.85 microM) as well as their DNA synthesis (IC50 = 1 microM). The possible role of lipoxygenase inhibition in the proliferation of leukemic cells is discussed.

Flavonoid glycosides from Erica cinerea

Abstract The new glycoside, limocitrin 4′-glucoside has been characterized from fresh flowers of Erica cinerea , together with the known compounds apigenin, apigenin 7-glucoside, quercetin 3-glucoside and 3-galactoside, gossypetin 8-methyl ether 3-glucoside and 3-galactoside as well as limocitrin 3-glucoside. Structural elucidation was achieved mainly by 1 H and 13 C NMR spectroscopy.

Norterpenoid and sesquiterpenoid glucosides from Juniperus phynicea and Galega officinalis

Abstract Four norterpenoid glucosides have been characterized along with a sesquiterpenoid glucoside from the aerial parts of Juniperus phynicea and Galega officinalis . Among these five compounds, two norterpenoids which are 3-oxo-a-ionol glucosides named junipeionoloside and 6-hydroxy-junipeionoloside are new natural products. These new norterpenoids were isolated from Juniperus phynicea together with an other known norterpenoid glucoside: roseoside and a sesquiterpenoid glucoside: dihydrophaseic acid 4′- O - β - d -glucopyranoside. We also have identified from Galega officinalis a rare norterpenoid glucoside: dearabinosyl pneumonanthoside.

Dihydroflavonol glycosides from Rhododendron ferrugineum

Abstract Chemical investigation of the leaves and the flowers of Rhododendron ferrugineum afforded six known flavonoids: hyperoside, myricetin 3-O-β-galactopyranoside, kaempferol 3-O-(6″-O-acetyl)-glucoside, quercetin 3-O-(6″-O-acetyl)-glucoside, quercetin 3-O-(6″-O-acetyl)-galactoside, quercetin 3-O-(3″,6″-O-diacetyl)-galactoside and two new dihydroflavonol glycosides: trans-taxifolin 3-O-α-arabinopyranoside and cis-taxifolin 3-O-α-arabinopyranoside.

Three phenylpropanoids from Juniperus phœnicea

Abstract Three new compounds, junipediol A [2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-diol], junipediol A 8-glucoside [1-β- d -glucosyloxy-2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-diol] and junipediol B 8-glucoside [1-β- d -glucosyloxy-2-(3,4-methylenedioxyphenyl)-propane-1,3-diol], have been isolated from acetone and methanolic extracts of the aerial parts of Juniperus phœnicea . The structural elucidation of these new natural products was achieved mainly by UV, mass and 1 H and 13 C NMR spectroscopy.

Quercetin 3-[triacetylarabinosyl(1→6)galactoside] and chromones from Calluna vulgaris☆

The new quercetin 3-[2,3,4-triacetyl-alpha-L-arabinopyranosyl (1-->6)-beta-D-galactoside] has been isolated along with 5,7-dihydroxychromone and 5,7-dihydroxychromone 7-beta-D-glucoside from fresh flowers of Calluna vulgaris. Structural elucidation of these natural products was achieved mainly by 1H and 13C NMR.

Further flavonoid glycosides from Calluna vulgaris

Abstract The new quercetin 3-[2,3,4-triacetyl-α- l -arabinosyl (1→ 6)-β- d -glucoside] has been isolated together with the dihydroflavonol glucoside c

Flavone and flavonol glycosides from Calluna vulgaris

Abstract The new flavonoid glycosides, apigenin 7-(2-acetyl 6-methyl)glucuronide and kaempferol 3-triacet-ylarabinosyl (l → 6)glucoside have been isolated from flowers of Calluna vulgaris and characterized by chemical and spectroscopic means.

A phloracetophenone glucoside from Rhododendron ferrugineum

Abstract The new phloracetophenone 4′-glucoside was isolated from dried flowers of Rhododendron ferrugineum L.