Albrecht Haase

Anticancer drug delivery system based on calcium carbonate particles loaded with a photosensitizer.

In photodynamic therapy (PDT), photosensitizers are required to arrive in high concentrations at selective targets like cancer cells avoiding toxicity in healthy tissue. In this work, we propose the application of porous calcium carbonate carriers in the form of polycrystalline vaterite for this task. We investigated the loading efficiency for the photosensitizer Photosens in vaterite micro- and nanocarriers. A possible release mechanism depending on the surrounding pH was studied, showing a fast degradation of the carriers in buffers below pH7. These results hold out the prospect of a novel PDT drug delivery system. Variation of particle size or additional coatings allow custom-design of workload release curves. An intrinsic cancer-sensitivity can be expected from the pH-dependent release in the acidic microenvironment of cancer tissue.

Possibility of single-atom detection on a chip

We investigate the optical detection of single atoms held in a microscopic atom trap close to a surface. Laser light is guided by optical fibers or optical microstructures via the atom to a photodetector. Our results suggest that with present-day technology microcavities can be built around the atom with sufficiently high finesse to permit unambiguous detection of a single atom in the trap with 10 µs of integration. We compare resonant and nonresonant detection schemes and discuss the requirements for detecting an atom without causing it to undergo spontaneous emission.

Trapping and manipulating neutral atoms with electrostatic fields

We report on experiments with cold thermal (7)Li atoms confined in combined magnetic and electric potentials. A novel type of three-dimensional trap was formed by modulating a magnetic guide using electrostatic fields. We observed atoms trapped in a string of up to six individual such traps, a controlled transport of an atomic cloud over a distance of 400 microm, and a dynamic splitting of a single trap into a double well potential. Applications for quantum information processing are discussed.

Heralded single-photon absorption by a single atom

The absorption of one photon of an entangled pair by a lone trapped atom is identified by a correlation between the atomic absorption process and the detection of the second photon.

Tunable narrowband entangled photon pair source for resonant single-photon single-atom interaction.

We present a tunable, frequency-stabilized, narrow-bandwidth source of frequency-degenerate, entangled photon pairs. The source is based on spontaneous parametric downconversion in periodically poled KTiOPO(4). Its wavelength can be stabilized to 850 or 854 nm, thus allowing to address two transitions in (40)Ca(+) ions. Its output bandwidth of 22 MHz coincides with the absorption bandwidth of the calcium ions. Its spectral power density is 1.0 generated pairs/(s MHz mW).

Tailored intracellular delivery via a crystal phase transition in 400 nm vaterite particles

Porous vaterite containers of 400 nm size are studied with respect to intracellular drug delivery applications. A generic crystal phase transition from vaterite to calcite serves as a novel payload release mechanism, which reveals a delayed burst-release. This will permit control of the pharmacokinetics allowing for applications like preventive drug administration or scheduled application of pharmaceuticals during long term therapy. Experiments with two types of payloads, providing different molecular weights and zeta-potentials, demonstrate a flexible way of tailoring the payload delivery time via the molecular properties of the cargo. A dual in vitro cellular uptake experiment with human ovarian carcinoma cells ES2 and human fibroblasts MRC5 shows no cytotoxicity, no influence on cell viability, and fast penetration of substance-loaded containers into cells. Flow cytometry analysis proves high uptake rates and 3D microscopy analysis reveals the intracellular distribution.

Searching for anatomical correlates of olfactory lateralization in the honeybee antennal lobes: a morphological and behavioural study.

The honeybee, Apis mellifera L. (Hymenoptera: Apidae), has recently become a model for studying brain asymmetry among invertebrates. A strong lateralization favouring the right antenna was discovered in odour learning and short-term memory recall experiments, and a lateral shift favouring the left antenna for long-term memory recall. Corresponding morphological asymmetries have been found in the distribution of olfactory sensilla between the antennae and confirmed by electrophysiological odour response measurements in isolated right and left antennae. The aim of this study was to investigate whether a morphological asymmetry can be observed in the volume of the primary olfactory centres of the central nervous system, the antennal lobes (ALs). Precise volume measurements of a subset of their functional units, the glomeruli, were performed in both sides of the brain, exploiting the advantages of two-photon microscopy. This novel method allowed minimal invasive acquisition of volume images of the ALs, avoiding artefacts from brain extraction and dehydration. The study was completed by a series of behavioural experiments in which response asymmetry in odour recall following proboscis extension reflex conditioning was assessed for odours, chosen to stimulate strong activity in the same glomeruli as in the morphological study. The volumetric measurements found no evidence of lateralization in the investigated glomeruli within the experimental limits. Instead, in the behavioural experiments, a striking odour dependence of the lateralization was observed. The results are discussed on the basis of recent neurophysiological and ethological experiments in A. mellifera.

Asymmetric neural coding revealed by in vivo calcium imaging in the honey bee brain.

Left–right asymmetries are common properties of nervous systems. Although lateralized sensory processing has been well studied, information is lacking about how asymmetries are represented at the level of neural coding. Using in vivo functional imaging, we identified a population-level left–right asymmetry in the honey bees primary olfactory centre, the antennal lobe (AL). When both antennae were stimulated via a frontal odour source, the inter-odour distances between neural response patterns were higher in the right than in the left AL. Behavioural data correlated with the brain imaging results: bees with only their right antenna were better in discriminating a target odour in a cross-adaptation paradigm. We hypothesize that the differences in neural odour representations in the two brain sides serve to increase coding capacity by parallel processing.

Detecting magnetically guided atoms with an optical cavity

We show that a low-finesse cavity can be efficient for detecting neutral atoms. The low finesse can be compensated for by decreasing the mode waist of the cavity. We have used a near-concentric resonator with a beam waist of 12 microm and a finesse of only 1100 to detect magnetically guided Rb atoms with a detection sensitivity of 0.1 atom in the mode volume. For future experiments on single-atom detection and cavity QED applications, it should be beneficial to use miniaturized optical resonators integrated on atom chips.

An entangled photon source for resonant single-photon?single-atom interaction

We present the development and operation of a tunable, frequency-stabilized, narrow-bandwidth source of entangled photon pairs, which can be tuned to the two D–P transitions in Ca+ ions at 850 and 854 nm. The source is based on spontaneous parametric down-conversion in periodically poled KTiOPO4 (PPKTP) followed by tunable optical filters. Its output bandwidth of 22 MHz coincides with the absorption bandwidth of the calcium ions. Its spectral power density is 1.0 generated pairs (s MHz mW)−1. Here, we report details of the setup which was first described in Haase et al (2009 Opt. Lett. 34 55).