Alcyr Oliveira

Motor Improvement Using Motion Sensing Game Devices for Cerebral Palsy Rehabilitation

ABSTRACT The authors aimed to investigate the effects of an intervention based on interactive game set with the movement sensor Kinect on children with cerebral palsy (CP). Twenty-eight participants were recruited. Their age was between 3 and 12 years old, and rated as level I, II, or III on the Gross Motor Function Classification System. They played two games from the Xbox 360 Kinect system and were evaluated using the Gross Motor Function Measure (GMFM) after a period of 8 weeks. The intervention led to significant motor function improvement as increase of the global scores on the GMFM (p < .001). Rehabilitation interventions using the Xbox 360 Kinect interactive games may represent useful tools for children with CP.

The footfault test as a screening tool in the 6-hydroxydopamine rat model of Parkinson's disease.

The administration of 6-hydroxydopamine (6-OHDA) into the nigrostriatal pathway is a rat model of Parkinsons disease (PD). The footfault test is a behavioural task in which rodents have their motor functions assessed. Here, we observed that unilaterally 6-OHDA-lesioned animals show a context-induced ipsilateral rotational behaviour when placed on the footfault apparatus for 3 min and this may be used as index to detect lesioned animals. Our results showed a sensitivity and specificity of 100% for lesions higher than 94% and 64%, respectively (ROC curve: AUC=0.988). A binary logistic regression model showed an expB=1.116 (95% CI, 1.007-1.236) and C=-9.081+/-4.554 (p=0.046) using the nigral tyrosine hidroxylase immunocontent as standard (each unit represents a 10%-lesion extension). Additionally, the footfault test was more sensitive than apomorphine challenging at 1mg/kg when these tests were carried out days apart and it was less sensitive than methylphenidate at 40 mg/kg (sign test, p<0.05). Therefore, the footfault test may be very useful in the PD animal model for screening animals since it is fast and simple and it does not require a drug to induce rotational activity.

Yoga and the Interplay between Attentional Load and Emotion Interference

This study compared 45 yoga practitioners (M age = 29.7yr., SD = 6.4) and 45 matched controls (M age = 29.3yr., SD = 6.2) on the performance of a behavioral task that assessed negative emotion interference during a high- and a low-attentional demand condition, as well as on state and trait anxiety scores. Outcomes were also compared between beginner and advanced practitioners. For the behavioral task, the final sample comprised 36 yoga and 38 control participants. The yoga group presented lower emotion interference in the high attentional condition, compared to the low attentional condition; rated emotional images as less unpleasant, compared to controls; and reported lower state and trait anxiety scores relative to controls. Also, emotion interference in the low attentional condition was lower among advanced practitioners and state anxiety was lower among practitioners attending more than two weekly yoga classes. The results suggested that yoga may help improve self-regulatory skills and lower anxiety. The psychological mechanisms underlying the relationship between yoga and emotion regulation should be further investigated in longitudinal studies.

Virtual reality technology for rehabilitation of cerebral palsy: a literature review

Cerebral palsy (CP) is a non-progressive encephalopathy that causes changes in muscle tone, posture and movement. The importance of early rehabilitation intervention is widely recognized, and new treatment tools such as computational technologies have been added for rehabilitation. The use of computational virtual reality games raises the possibility to control the training intensity as well as to observe the progress and direct monitoring control over patients’ activities. Additionally, some studies have shown the effectiveness of these systems in improving the management of tasks for patients with various levels of encephalopathy. Based on the assumptions above, the objective of this review was to discuss the literature on the use of such technologies in the rehabilitation of CP patients. The potential use of VR in rehabilitation of CP patients is clear. There is, however, a reduced number of randomized trials to sustain whether these methods may effectively act as a complement for physical and/or neuro-cognitive rehabilitation or work as a replacement for the traditional rehabilitation methods.

Mesenchymal stem cell transplantation and aerobic exercise for Parkinson’s disease: therapeutic assets beyond the motor domain

Abstract Parkinson’s disease (PD) is a very common neurodegenerative condition in which both motor and nonmotor deficits evolve throughout the course of the disease. Normally characterized as a movement disorder, PD has been broadly studied from a motor perspective. However, mild to moderate cognitive deficits began to appear in the early phases of the disease, even before motor disturbances actually manifest, and continue to progress relentlessly. These nonmotor manifestations are also a source of detriment to the patients’ already strained functionality and quality of life, and pose a therapeutic challenge seeing that replacing therapies have had conflicting results. Considering that the currently approved therapies can hardly be considered curative, efforts to find therapeutic approaches with an actual disease-modifying quality and capable of addressing not only motor but also cognitive dysfunctions are clearly needed. Among possible alternatives with such attribute, mesenchymal stem cell transplantation and exercise are worth highlighting given their common neuroprotective, neuroplastic, and immunomodulatory properties. In this paper, we will summarize the existent literature on the topic, focusing on the mechanisms of action through which these two approaches might beget therapeutic benefits for PD beyond the commonly assessed motor dysfunctions, alluding, at the same time, toward a potential synergic association of both therapies as an optimized approach for PD.

Association of GPX1 and GPX4 polymorphisms with episodic memory and Alzheimer’s disease

It is well established that healthy aging, mild cognitive impairment (MCI), and Alzheimers disease (AD) are associated with substantial declines in episodic memory. However, there is still debate about the roles of GPX1 and GPX4 polymorphisms. The aim of this study was to investigate the association of rs1050450 and rs713041 polymorphisms with memory. This research was composed of a cross-sectional study (334 subjects) and a case-control study (108 healthy controls and 103 with AD-NINCDS/ARDA, DSM-IV-TR criteria). For the association of the genetic polymorphisms with memory or cognitive loss, the phenotypes were analyzed as follows: 1) each memory as a quantitative trait; 2) presence of deficit on a specific memory; 3) presence of MCI; 4) presence of AD. To assess verbal learning and the ability to store new information, we used the Rey Verbal Learning Test. Scores were recorded as a function of age as in the WMS-R testing battery. DNA was obtained from whole blood, and genotypes for GPX1 (rs1050450) and GPX4 (rs713041) were detected by allelic discrimination assay using TaqMan® MGB probes on a real-time PCR system. GPX1 TT homozygotes had lower long-term visual memory scores than CC/CT group (-0.28 ± 1.03 vs. 0.13 ± 1.03, respectively, p = 0.017). For the GPX4 rs713041, the frequency of the TT genotype was higher in the group with normal scores than in the group with long-term visual memory deficits (p = 0.025). In a multivariate logistic regression, GPX1 CC homozygotes had a 2.85 higher chance of developing AD (OR = 2.85, CI95% = 1.04-7.78, p = 0.041) in comparison to the reference genotype. No significant differences were observed regarding the MCI group between genetic variants. This study is one of the first to show that polymorphisms in GPX1 and GPX4 are significantly associated with episodic memory and AD in a South Brazilian population.

Green Juice in Human Metabolism: A Randomized Trial

ABSTRACT Objective: Fruits and vegetables contain many compounds presenting potential antioxidant activity. The objective of this study was to evaluate the effect of a green juice recipe in adult metabolism in order to identify new preventive dietary sources. Method: This was a single-blind randomized controlled clinical trial. Recruitment and data were, respectively, made and collected at the Universidade Federal de Ciências da Saúde de Porto Alegre. Individuals who met all the inclusion criteria during the period of recruitment were included. Green juice (experimental group) or placebo (control group) were consumed from Monday to Friday between 8 and 9 am, in the amount of 300 mL for 60 days (except Saturdays and Sundays). To verify the effect of green juice on metabolism, the following were evaluated: (a) glycemia, plasma lipid profile, renal and liver functions, redox profile, and antioxidant enzymes; (b) anthropometry; and (c) well-being and anxiety. Results: This study included 14 participants in the test group (juice group) and 13 controls (placebo group), with mean ages of 31.07 and 30.15 years, respectively. We did not observe a significant difference between the treatments. Dietary properties of vegetable and fruit juices are an area of significant interest. Conclusions: Together with an analysis of previous works, we suggest that green juice did not cause an improvement in metabolic function and there is a need for further research on this issue, mainly through different interventions and other samples.

Stem cells transplantation for the treatment of cognitive deficits in Parkinson’s disease.

Parkinson’s disease (PD) is a progressive neurodegenerative syndrome classically characterized by motor dysfunctions such as tremor, slowness of movement, postural instability, and rigidity. However, an additional decline in cognitive function to different degrees of compromise may coexist. Many therapeutic approaches have been established to treat and delay the progression of these impairments. Most of them are not restorative and just aim to keep the functions on. Stem cell (SC) transplantation is a promising treatment for many neurological conditions, and PD motor symptoms have been particularly targeted with this approach. Nonetheless, it is also potentially effective for cognitive deficits. By boosting the release of neurotropic factors, enhancing the synaptic density and plasticity, and improving the survival of endangered neurons and replacing those already lost, it targets underlying pathological mechanisms and therefore holds potential curative properties. The present minireview emphasizes the current evidence that encourages the use of this therapeutic approach for the treatment of PD-related cognitive deficits. All the same, this area of study require more and PD-specific studies that will help to elucidate the mechanisms of action through which this therapy could bring a clinical improvement, thereby assessing the real restorative potential of stem cell transplantation in this condition.

Modelos animales de degeneración cognitiva y uso de transplantes neurales de células madre como terapia

There are few animal models of cognitive decline that could be considered as prominent as the cholinergic lesion model of Alzheimer’s disease. Alzheimer’s disease is a neurodegenerative disorder associated with aging with accentuated progressive cognitive impairment. Its multiple complex sequential and interactive neural pathways may lead to the understanding that a single agent intervention might be insufficient to impact the course of this disease. The current pharmacological treatment is palliative and, although it may slow down the disease’s progression, it still cannot offer cure. The combination of drugs and antioxidant substances has been investigated, nevertheless there are few studies demonstrating optimal and consistent results based on this combination. Thus, the purpose of this paper is to highlight cell-based therapies as prospective treatment of peripheral and central nervous system disorders.

Tecnología computacional de la realidad virtual aplicada a la rehabilitación de la parálisis cerebral: una revisión de la literatura

Cerebral palsy (CP) is a non-progressive encephalopathy that causes changes in muscle tone, posture and movement. The importance of early rehabilitation intervention is widely recognized, and new treatment tools such as computational technologies have been added for rehabilitation. The use of computational virtual reality games raises the possibility to control the training intensity as well as to observe the progress and direct monitoring control over patients’ activities. Additionally, some studies have shown the effectiveness of these systems in improving the management of tasks for patients with various levels of encephalopathy. Based on the assumptions above, the objective of this review was to discuss the literature on the use of such technologies in the rehabilitation of CP patients. The potential use of VR in rehabilitation of CP patients is clear. There is, however, a reduced number of randomized trials to sustain whether these methods may effectively act as a complement for physical and/or neuro-cognitive rehabilitation or work as a replacement for the traditional rehabilitation methods.