Alexandru Petculescu

Peculiarity of the mammal associations from the Upper Pleistocene (Dobrogea, Romania)

Paleontological studies of the mammals from the Upper Pleistocene at Dobrogea are distinct in comparison with other mammal associations from Romania. The paleoenvironment from the last glacial cycle (Wurm) is reconstructed on the basis of the mammal cave fossils discovered in caves at Dobrogea. Research in karst deposits from the last glacial cycle is based on systematic surveying of the most important paleontological sites.

Novel approach to microbiological air monitoring in show caves

Air microbial pollution in touristic areas poses a risk for both the integrity of an ecosystem and human health. Microbiological monitoring together with environmental parameters monitoring allows for the assessment of the impacts and formulation of sound management decisions to protect humans and ecosystems. Four show caves from the Carpathian Mountains were selected for our study. The caves were sampled monthly to obtain an overview of the changes that occur over a yearly cycle. For the microbial monitoring, we used RIDA®COUNT test plates, while the environmental parameters were monitored with a variety of devices. Second and third generations of microbes extracted from the plates were grown on specific media for the purpose of 16S rRNA and 18S rRNA extraction and taxa identification. The bacterial communities identified in the air samples in the four investigated show caves were dominated by Staphylococcus, while regarding the fungi communities, Penicillium was more likely to occur in the touristic part of the caves and Cladosporium in the non-visited passages. Together with data on number of visitors, number of bats and radon levels we were able understand the impact of tourists on the cave environment and to generate microbiological risk maps for human health. This type of comprehensive study can be used not only to protect the integrity of a touristic area from the impacts caused by the introduction of allochthonous organic matter, but also for the protection of the tourists and guides from potential pathogenic taxa.