Alice E. Thackray

Acute High-Intensity Interval Running Reduces Postprandial Lipemia in Boys

INTRODUCTION Acute moderate-intensity exercise reduces postprandial lipemia in boys. However, the effect of high-intensity exercise has not been investigated. This study examined the effect of low-volume, high-intensity interval running (HIIR) on postprandial plasma triacylglycerol (TAG) concentrations. METHODS Fifteen healthy, active boys (means ± SD; age = 11.8 ± 0.4 yr, body mass = 42.8 ± 8.0 kg, peak oxygen uptake [VO2] = 55 ± 6 mL · kg(-1) · min(-1)) completed two 2-d trials in a counterbalanced, crossover design separated by 14 d. On day 1, participants rested (CON) or completed 10 × 1 min running intervals at 100% maximal aerobic speed, determined from an incremental peak VO2 test, with 1 min recovery between intervals (HIIR). On day 2, capillary blood samples were taken in the fasted state and at predetermined intervals throughout the 6.5-h postprandial period while participants rested. A standardized breakfast was consumed at 0800 h, immediately after the fasting sample, and a standardized lunch meal was consumed at 1200 h. RESULTS Differences in fasting plasma TAG concentration were small to moderate (95% confidence interval [CI] = -0.11 to 0.01, effect size [ES] = 0.40). Postprandial TAG concentration was lower during HIIR compared with CON (95% CI = -0.19 to -0.02, ES = 0.58). The total area under the TAG concentration versus time curve was lower after HIIR compared with CON (5.2 ± 1.1 vs 5.8 ± 1.5 mmol(-1) · L 6.5 h; 95% CI = -1.18 to -0.12, ES = 0.50). CONCLUSION This is the first study to show that low-volume HIIR attenuates postprandial TAG concentration in healthy, active 11- to 12-yr-old boys.

Exercise, appetite and weight control: are there differences between men and women?

Recent years have witnessed significant research interest surrounding the interaction among exercise, appetite and energy balance, which has important implications for health. The majority of exercise and appetite regulation studies have been conducted in males. Consequently, opportunities to examine sex-based differences have been limited, but represent an interesting avenue of inquiry considering postulations that men experience greater weight loss after exercise interventions than women. This article reviews the scientific literature relating to the acute and chronic effects of exercise on appetite control in men and women. The consensus of evidence demonstrates that appetite, appetite-regulatory hormone and energy intake responses to acute exercise do not differ between the sexes, and there is little evidence indicating compensatory changes occur after acute exercise in either sex. Limited evidence suggests women respond to the initiation of exercise training with more robust compensatory alterations in appetite-regulatory hormones than men, but whether this translates to long-term differences is unknown. Current exercise training investigations do not support sex-based differences in appetite or objectively assessed energy intake, and increasing exercise energy expenditure elicits at most a partial energy intake compensation in both sexes. Future well-controlled acute and chronic exercise studies directly comparing men and women are required to expand this evidence base.

Acute effect of exercise intensity and duration on acylated ghrelin and hunger in men

Acute exercise transiently suppresses the orexigenic gut hormone acylated ghrelin, but the extent to which exercise intensity and duration determine this response is not fully understood. The effects of manipulating exercise intensity and duration on acylated ghrelin concentrations and hunger were examined in two experiments. In experiment one, nine healthy males completed three, 4-h conditions (control, moderate-intensity running (MOD) and vigorous-intensity running (VIG)), with an energy expenditure of ~2.5 MJ induced in both MOD (55-min running at 52% peak oxygen uptake (V.O2peak)) and VIG (36-min running at 75% V.O2peak). In experiment two, nine healthy males completed three, 9-h conditions (control, 45-min running (EX45) and 90-min running (EX90)). Exercise was performed at 70% V.O2peak In both experiments, participants consumed standardised meals, and acylated ghrelin concentrations and hunger were quantified at predetermined intervals. In experiment one, delta acylated ghrelin concentrations were lower than control in MOD (ES = 0.44, P = 0.01) and VIG (ES = 0.98, P < 0.001); VIG was lower than MOD (ES = 0.54, P = 0.003). Hunger ratings were similar across the conditions (P = 0.35). In experiment two, delta acylated ghrelin concentrations were lower than control in EX45 (ES = 0.77, P < 0.001) and EX90 (ES = 0.68, P < 0.001); EX45 and EX90 were similar (ES = 0.09, P = 0.55). Hunger ratings were lower than control in EX45 (ES = 0.20, P = 0.01) and EX90 (ES = 0.27, P = 0.001); EX45 and EX90 were similar (ES = 0.07, P = 0.34). Hunger and delta acylated ghrelin concentrations remained suppressed at 1.5 h in EX90 but not EX45. In conclusion, exercise intensity, and to a lesser extent duration, are determinants of the acylated ghrelin response to acute exercise.

Acute Exercise and Postprandial Lipemia in Young People

Exaggerated postprandial triacylglycerol concentrations (TAG) independently predict future cardiovascular events. Acute exercise and diet interventions attenuate postprandial TAG in adults. This paper aims to examine the exercise postprandial lipemia studies published to date in young people. Nine studies satisfied the inclusion criteria adopted for this summary. The majority of studies are in boys (22% girls) and have shown a single ~60-min session of moderate-intensity exercise, performed 12-18 hours before a standardized meal, reduces postprandial TAG. Manipulations of exercise duration and intensity suggest an exercise energy expenditure dose-dependent response is not supported directly in healthy young people. Studies investigating alternative exercise bouts have reported lower postprandial TAG after simulated intermittent games activity, high-intensity interval running and cumulative 10-min blocks over several hours, which may appeal to the spontaneous physical activity habits of young people. Although extension of these initial findings is warranted, exercise may be an effective strategy to promote regular benefits in TAG metabolism in children and adolescents; this may contribute to an improved cardiovascular disease risk profile early in life.

Acute effects of exercise on appetite, ad libitum energy intake and appetite-regulatory hormones in lean and overweight/obese men and women

Background:Acute exercise does not elicit compensatory changes in appetite parameters in lean individuals; however, less is known about responses in overweight individuals. This study compared the acute effects of moderate-intensity exercise on appetite, energy intake and appetite-regulatory hormones in lean and overweight/obese individuals.Methods:Forty-seven healthy lean (n=22, 11 females; mean (s.d.) 37.5 (15.2) years; 22.4 (1.5) kg m−2) and overweight/obese (n=25, 11 females; 45.0 (12.4) years, 29.2 (2.9) kg m−2) individuals completed two, 8 h trials (exercise and control). In the exercise trial, participants completed 60 min treadmill exercise (59 (4)% peak oxygen uptake) at 0–1 h and rested thereafter while participants rested throughout the control trial. Appetite ratings and concentrations of acylated ghrelin, peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) were measured at predetermined intervals. Standardised meals were consumed at 1.5 and 4 h and an ad libitum buffet meal was provided at 7 h.Results:Exercise suppressed appetite (95% confidence interval (CI) −3.1 to −0.5 mm, P=0.01), and elevated delta PYY (95% CI 10 to 17 pg ml−1, P<0.001) and GLP-1 (95% CI 7 to 10 pmol l−1, P<0.001) concentrations. Delta acylated ghrelin concentrations (95% CI −5 to 3 pg ml−1, P=0.76) and ad libitum energy intake (95% CI −391 to 346 kJ, P=0.90) were similar between trials. Subjective and hormonal appetite parameters and ad libitum energy intake were similar between lean and overweight/obese individuals (P⩾0.27). The exercise-induced elevation in delta GLP-1 was greater in overweight/obese individuals (trial-by-group interaction P=0.01), whereas lean individuals exhibited a greater exercise-induced increase in delta PYY (trial-by-group interaction P<0.001).Conclusions:Acute moderate-intensity exercise transiently suppressed appetite and increased PYY and GLP-1 in the hours after exercise without stimulating compensatory changes in appetite in lean or overweight/obese individuals. These findings underscore the ability of exercise to induce a short-term energy deficit without any compensatory effects on appetite regardless of weight status.

Individual variation in hunger, energy intake and ghrelin responses to acute exercise

Purpose This study aimed to characterize the immediate and extended effect of acute exercise on hunger, energy intake, and circulating acylated ghrelin concentrations using a large data set of homogenous experimental trials and to describe the variation in responses between individuals. Methods Data from 17 of our groups experimental crossover trials were aggregated yielding a total sample of 192 young, healthy males. In these studies, single bouts of moderate to high-intensity aerobic exercise (69% ± 5% V˙O2 peak; mean ± SD) were completed with detailed participant assessments occurring during and for several hours postexercise. Mean hunger ratings were determined during (n = 178) and after (n = 118) exercise from visual analog scales completed at 30-min intervals, whereas ad libitum energy intake was measured within the first hour after exercise (n = 60) and at multiple meals (n = 128) during the remainder of trials. Venous concentrations of acylated ghrelin were determined at strategic time points during (n = 118) and after (n = 89) exercise. Results At group level, exercise transiently suppressed hunger (P < 0.010, Cohens d = 0.77) but did not affect energy intake. Acylated ghrelin was suppressed during exercise (P < 0.001, Cohens d = 0.10) and remained significantly lower than control (no exercise) afterward (P < 0.024, Cohens d = 0.61). Between participants, there were notable differences in responses; however, a large proportion of this spread lay within the boundaries of normal variation associated with biological and technical assessment error. Conclusion In young men, acute exercise suppresses hunger and circulating acylated ghrelin concentrations with notable diversity between individuals. Care must be taken to distinguish true interindividual variation from random differences within normal limits.

Exercise energy expenditure and postprandial lipemia in girls

PURPOSE This study aimed to examine the effect of 30 and 60 min of moderate-intensity treadmill walking on postprandial triacylglycerol concentrations ([TAG]) in healthy girls. METHODS Eighteen 10- to 14-yr-old girls (mean ± SD; body mass = 48 ± 11 kg, body fat = 19.0 ± 4.6%, peak oxygen uptake [V˙O2] = 47 ± 6 mL·kg·min) completed three 2-d trials in a counterbalanced crossover design, each separated by 14 d. On day 1, they rested (CON) or completed 30 min (EX30) or 60 min (EX60) of intermittent treadmill exercise at 56% peak V˙O2, inducing energy expenditures of 777 and 1536 kJ (186 and 367 kcal), respectively. On day 2, after a 12-h fast, a capillary blood sample was taken for fasting [TAG] before a high-fat milkshake (80 kJ·kg(-1) body mass) was consumed. Further blood samples were taken hourly over a 6-h postprandial rest period for [TAG]. ANOVA and Students t-tests were used to analyze the data. RESULTS Fasting [TAG] was lower in EX60 than CON (95% confidence interval [CI] = -0.36 to 0.04, effect size (ES) = 0.41) and EX30 (95% CI = -0.47 to 0.04, ES = 0.46); all group mean concentrations were low (≤ 0.90 mmol·L(-1)). The main effect for condition revealed differences in postprandial [TAG] over time (ES = 0.36). The EX60 total area under the [TAG] versus time curve was lower than CON (95% CI = -2.66 to -0.04, ES 0.40) and EX30 (95% CI = -2.11 to 0.15, ES = 0.30); CON and EX30 were similar (95% CI = -1.44 to 0.71, ES = 0.10). CONCLUSION This study demonstrates that 60 min but not 30 min of moderate treadmill exercise, with a gross energy expenditure of 1536 kJ (367 kcal), attenuated postprandial [TAG] in girls.

High-Intensity Running and Energy Restriction Reduce Postprandial Lipemia in Girls.

PURPOSE This study examined the potency of combining acute high-intensity exercise and energy intake restriction on postprandial triacylglycerol concentrations ([TAG]) in healthy girls. METHODS Sixteen 11- to 13-yr-old girls (mean (SD) body mass, 45.1 (7.6) kg; peak oxygen uptake (V˙O2), 43 (6) mL·kg(-1)·min(-1)) completed three 2-d conditions in a counterbalanced crossover design separated by 14 d. On day 1, participants completed 10 × 1-min interval runs (HIIR) or 5 × 1-min interval runs combined with 0.82 (0.19) MJ energy intake restriction (HIIR-ER) or rested (CON). Exercise was completed at 100% maximal aerobic speed determined from an incremental peak V˙O2 test, with 1-min recovery between intervals. On day 2, capillary blood samples were taken in the fasted state and at predetermined intervals throughout the 6.5-h postprandial period. A standardized breakfast and lunch were consumed immediately and 4 h after the fasting sample, respectively. RESULTS Based on ratios of the geometric means (95% confidence intervals (CI) for ratios), fasting [TAG] was 16% and 8% lower than CON in HIIR (-24% to -7%; effect size (ES), 0.49; P = 0.002) and HIIR-ER (-17% to 1%; ES, 0.24; P = 0.09), respectively; HIIR was 8% lower than HIIR-ER (-17% to 1%; ES, 0.25; P = 0.08). The total area under the [TAG] versus time curve was 10% and 9% lower than CON in HIIR (-16% to -3%; ES, 0.30; P = 0.01) and HIIR-ER (-15% to -2%; ES, 0.28; P = 0.01), respectively; HIIR-ER and HIIR were similar (-1%; -8% to 6%; P = 0.80). CONCLUSIONS Manipulations of HIIR and ER reduce postprandial [TAG] in girls. The magnitude of effect was marginally, although not meaningfully, greater after HIIR than that after HIIR-ER.

Acute Effects of Energy Deficit Induced by Moderate-Intensity Exercise or Energy-Intake Restriction on Postprandial Lipemia in Healthy Girls

Eleven healthy girls (mean ± SD: age 12.1 ± 0.6 years) completed three 2-day conditions in a counterbalanced, crossover design. On day 1, participants either walked at 60 (2)% peak oxygen uptake (energy deficit 1.55[0.20] MJ), restricted food energy intake (energy deficit 1.51[0.25] MJ) or rested. On day 2, capillary blood samples were taken at predetermined intervals throughout the 6.5 hr postprandial period before, and following, the ingestion of standardized breakfast and lunch meals. Fasting plasma triacylglycerol concentrations (TAG) was 29% and 13% lower than rest control in moderate-intensity exercise (effect size [ES] = 1.39, p = .01) and energy-intake restriction (ES = 0.57, p = .02) respectively; moderate-intensity exercise was 19% lower than energy-intake restriction (ES = 0.82, p = .06). The moderate-intensity exercise total area under the TAG versus time curve was 21% and 13% lower than rest control (ES = 0.71, p = .004) and energy-intake restriction (ES = 0.39, p = .06) respectively; energy-intake restriction was marginally lower than rest control (-10%; ES = 0.32, p = .12). An exercise-induced energy deficit elicited a greater reduction in fasting plasma TAG with a trend for a larger attenuation in postprandial plasma TAG than an isoenergetic diet-induced energy deficit in healthy girls.

Interindividual responses of appetite to acute exercise: a replicated crossover study

Purpose Acute exercise transiently suppresses appetite, which coincides with alterations in appetite-regulatory hormone concentrations. Individual variability in these responses is suspected, but replicated trials are needed to quantify them robustly. We examined the reproducibility of appetite and appetite-regulatory hormone responses to acute exercise and quantified the individual differences in responses. Methods Fifteen healthy, recreationally active men completed two control (60-min resting) and two exercise (60-min fasted treadmill running at 70% peak oxygen uptake) conditions in randomized sequences. Perceived appetite and circulating concentrations of acylated ghrelin and total peptide YY (PYY) were measured immediately before and after the interventions. Interindividual differences were explored by correlating the two sets of response differences between exercise and control conditions. Within-participant covariate-adjusted linear mixed models were used to quantify participant–condition interactions. Results Compared with control, exercise suppressed mean acylated ghrelin concentrations and appetite perceptions (all ES = 0.62–1.47, P < 0.001) and elevated total PYY concentrations (ES = 1.49, P < 0.001). For all variables, the standard deviation of the change scores was substantially greater in the exercise versus control conditions. Moderate-to-large positive correlations were observed between the two sets of control-adjusted exercise responses for all variables (r = 0.54–0.82, P ⩽ 0.036). After adjusting for baseline measurements, participant–condition interactions were present for all variables (P ⩽ 0.053). Conclusions Our replicated crossover study allowed, for the first time, the interaction between participant and acute exercise response in appetite parameters to be quantified. Even after adjustment for individual baseline measurements, participants demonstrated individual differences in perceived appetite and hormone responses to acute exercise bouts beyond any random within-subject variability over time.