Alla A. Buryakova

PNA-RELATED OLIGONUCLEOTIDE MIMICS AND THEIR EVALUATION FOR NUCLEIC ACID HYBRIDIZATION STUDIES AND ANALYSIS

DNA mimics containing phosphonate analogues of PNAs (pPNAs), particularly PNA-pPNA hybrids as well as hetero-oligomers consisted of pPNA units and PNA-like molecules on the base of trans-4-hydroxy-L-proline (HypNA) have been synthesized. The evaluation of their effectiveness in assays based on the hybridization technique in the comparison with natural oligonucleotides and classical PNAs has shown a high potential of these mimics as sensor molecules for nucleic acid based diagnostics and as molecular probes for mRNA isolation.

Synthesis of DNA analogues with novel carboxamidomethyl phosphonamide and glycinamide internucleoside linkages.

Thymidine oligonucleotide analogues with phosphodiester bonds fully substituted by carboxamidomethyl phosphonamide, or glycinamide linkages were synthesized on a solid support, and their hybridization properties toward DNA and RNA targets were determined by Tm analysis.

Synthesis and Properties of Some Morpholino Oligonucleotide Analogues

Abstract The solid phase synthesis of the three types of morpholino analogues of nucleic acids has been accomplished and their hybridization properties were evaluated.

Polyester and N-Methyl Analogues of Peptide Nucleic Acids: Synthesis and Hybridization Properties

Abstract The synthesis of a set of homo-thymine analogues of peptide nucleic acids containing ester or N-methylamide linkages as well as analogues derived from trans-4-hydroxy-L-proline and their hybrids has been accomplished, and their binding affinity to complementary DNA and RNA strands was evaluated.

Synthesis And Binding Study Of Phosphonate Analogues Of Pnas And Their Hybrids With Pnas

Abstract The preparation of monomers for the synthesis of phosphonate analogues of peptide nucleic acids containing the four natural nucleobases: thymine, cytosine, adenine and guanine, has been ccomplished. The monomers obtained were used for the automated online solid phase synthesis of pure phosphono-PNA oligomers as well as chimeras consisting of phosphono-PNA and PNA resudies. The hybridization properties of these oligonucleotide mimics to complementary DNA and RNA fragments were studied.

NOVEL OLIGONUCLEOTIDE ANALOGUES DERIVED FROM SERINE AND 4-HYDROXYPROLINE

Abstract A set of DNA analogues derived from serine and 4-hydroxyproline and containing carboxamidomethyl phosphonate and carboxamidophosphate bonds between monomeric units was synthesized.

CONVENIENT APPROACHES TO THE SYNTHESIS OF OLIGONUCLEOTIDE MACROCYCLES CONTAINING NON-NUCLEOTIDE LINKERS

Two convenient, practical routes to the synthesis of non-nucleotide bridged cyclic oligonucleotides have been developed. The first procedure included circularization of oligonucleotides by template-directed ligation on solid phase, while the second procedure involved preparation of a circular oligomer by non-template chemical ligation of a linear precursor in solution. Using these approaches, a series of single- and double-stranded cyclic oligonucleotides with non-nucleotide bridges has been synthesized.

Synthesis of Circular Oligonucleotide Conjugates

Abstract A new approach to the non-template synthesis of circular oligodeoxyribonucleotides containing flexible non-nucleotidic linkers has been developed. Using this technique a set of circular molecules representing triple helix forming, antisense and guanosine tetrad containing oligonucleotides has been obtained.