Alojzy Danch

Selenium levels in blood of upper Silesian population: evidence of suboptimal selenium status in a significant percentage of the population.

The selenium status and the relationship of whole-blood selenium and plasma homocysteine are reported for healthy human subjects living in Upper Silesia. A total of 1063 individuals (627 male and 436 female) examined for whole-blood selenium were subdivided into six groups according to age; the youngest included adolescents (n=143) aged 10–15 yr, and the oldest were centenarians (n=132). The mean Se content was relatively low (62.5±18.4 μg/L), and it tended to be higher in men (65.9±17.2 μg/L) than in women (57.5±18.9 μg/L). Selenium levels appeared to be age dependent, as the highest values were observed in young and middle-age adults (21–40 yr), whereas they were significantly lower in adolescents and in the elderly. In more than 40% of apparently healthy adults (aged 21–69 yr), the Se concentration was within the range 60–80 μg/L (i.e., below the lower limit of the nutritional adequacy range [80 μg/L]). A significant inverse correlation between whole-blood selenium and plasma total homocysteine was detected in a smaller population sample of middle-aged and elderly persons (n=204).

Effects of Electromagnetic Field on Free-Radical Processes in Steelworkers. Part II: Magnetic Field Influence on Vitamin A, E and Selenium Concentrations in Plasma

Effects of Electromagnetic Field on Free‐ Radical Processes in Steelworkers. Part II: Magnetic Field Influence on Vitamin A, E and Selenium Concentrations in Plasma: Andrzej Sobczak, et al. Department of General and Analytical Chemistry, Silesian School of Medicine, Poland—The aim of the study was the evaluation of the influence of electromagnetic fields (electric field strength of 20 V/m, f=50 Hz and magnetic field strength of 2 A/m, f=50 Hz) on the concentrations of vitamin A, vitamin E (a‐ and y‐tocopherol), and selenium, which represent non‐enzymatic antioxidants, in the plasma of steelworkers exposed to such a field. The cohort was divided into 3 study groups: group 1, workers employed for less than 3 yr; group 2, employed for 3‐ 10 yr; and group 3, employed for more than 10 years. Analysis of the results obtained revealed a statistically significant decrease in plasma vitamin A, α‐, and γ‐ tocopherol concentrations in the study groups as well as a marked increase in plasma selenium concentrations in groups 2 and 3. These changes suggest that the electromagnetic fields studied cause an increase in free‐radical process activity in steelworkers.

Silicon metabolism. The interrelations of inorganic silicon (Si) with systemic iron (Fe), Zinc (Zn), and copper (Cu) pools in the rat.

The influence of silicon treatment on the levels of trace elements zinc (Zn), copper (Cu), and iron (Fe) in serum and tissues was studied in rats. The concentrations of silicon, iron, and zinc were estimated in samples of sera and tissues of rats receivingper os a soluble, inorganic silicon compound—sodium metasilicate nonahydrate (Na2SiO3·9H2O), dissolved in the drinking water. An increase of copper concentrations in liver and aortic walls in the experimental group was observed, with simultaneous reduction of zinc amounts in serum and all the tissue samples in the course of the experiment. The iron concentrations in the analyzed samples did not show any significant changes between both groups. The silicon levels in serum and in all the examined tissues were significantly higher in the tested group.The results provide evidence for the silicon interaction with copper and zinc, which could result in a number of metabolic process modifications, antiatheromatous activity among them.

Modulation of procainamide toxicity by selenium-enriched yeast in rats

Free radical processes are proposed to play a crucial role in the development of procainamide adverse effects. Therefore, selenium, as a potent antioxidant, may modified procainamide toxicity. To test this hypothesis plasma and liver thiobarbituric acid-reacting substances (TBARS), plasma antioxidant activity (AOA), erythrocyte and liver superoxide dismutase (SOD), catalase, as well as selenium-dependent glutathione peroxidase (Se-GPX) were determined in the following four groups of rats: selenium-treated (Se), procainamide-treated (P), procainamide and selenium-treated (P + Se), and control (C). Morphological studies of leukocytes [tested for lupus erythematosus (LE) cells] and liver were also made. Atypical, i.e. enlarged and swollen, leukocytes resulting from procainamide and selenium treatment were observed. These changes were found in four out of five rats in the Se group, eight out of ten in the P group, and in seven out of ten in the P + Se group. LE-like cells were observed in two rats in the P + Se group. A statistically significant decrease in plasma and liver TBARS by 20% and 36%, respectively, increased activity of SOD by 20%, catalase by 48% and Se-GPX by 15% in erythrocytes, and decreased activity of liver SOD by 17% and catalase by 22% were found in the P + Se group as compared to the P group. These results indicated that selenium exerted antioxidant effects on the procainamide-treated rats. However, selenium did not prevent the development of disturbances in leukocyte morphology, on the contrary, it possibly promoted the conversion of leukocytes to LE cells.