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Dive into the research topics where Ewa Kopieczna-Grzebieniak is active.

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Featured researches published by Ewa Kopieczna-Grzebieniak.


Metabolism-clinical and Experimental | 1992

Effect of hemodialysis on lipid peroxidation and antioxidant system in patients with chronic renal failure.

Michal Toborek; Tomasz Wasik; Marian Dróżdż; Mariusz Klin; Krystyna Magner-Wróbel; Ewa Kopieczna-Grzebieniak

Plasma lipid peroxidation, activity of erythrocyte superoxide dismutase (SOD) and catalase, and serum antioxidant activity (AOA) in uremic patients were examined before and after hemodialysis. An increased level of lipid peroxidation, a decreased serum AOA level, and elevated SOD and normal catalase activity before hemodialysis were observed in uremic patients compared with controls. Hemodialysis was found to produce increased lipid peroxidation, a simultaneous decrease of SOD activity, and lack of any changes in serum AOA and erythrocyte catalase. It is suggested that intensification of lipid peroxidation during hemodialysis could account for accelerated progress of atherosclerosis in patients with renal insufficiency.


Atherosclerosis | 1995

Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits

Michal Toborek; Ewa Kopieczna-Grzebieniak; Marian Dróżdż; Marian Wieczorek

Methionine is converted by the transmethylation/transsulfuration pathway to homocysteine which may exert atherogenic effects by several mechanisms, including lipid peroxidation. Therefore, the excessive dietary methionine may induce the development of atherosclerosis. To test this hypothesis, plasma and aortic thiobarbituric acid reactive substances (TBARS), as well as activities of aortic and erythrocyte superoxide dismutase (SOD), catalase and selenium-dependent glutathione peroxidase (GPX) were measured in rabbits fed a diet enriched with 0.3% methionine for 6 or 9 months. Histological examinations of aortas also were performed. Feeding rabbits a methionine-enriched diet for 6 or 9 months resulted in significant increases in plasma and aortic TBARS levels and aortic antioxidant enzyme activities. However, a decrease in plasma antioxidant activity (AOA) was observed. In erythrocytes, SOD activity increased, catalase remained normal and GPX decreased in the treated animals. Histological examination of aortas showed typical atherosclerotic changes, such as intimal thickening, deposition of cholesterol, and calcification in methionine-fed rabbits. These results confirm that high-methionine diet may induce atherosclerosis in rabbits and indicate disturbances in lipid peroxidation and antioxidant processes as possible mechanisms of its atherogenic influence.


Nutrition | 1996

Increased lipid peroxidation and antioxidant activity in methionine-induced hepatitis in rabbits

Michal Toborek; Ewa Kopieczna-Grzebieniak; Marian Dróżdż; Marian Wieczorek

The liver is especially susceptible to the toxic effects of methionine due to its role in sulfur amino acid metabolism. Therefore, the excessive amounts of this amino acid may induce liver damage. To test the mechanisms of methionine-related hepatotoxicity, liver thiobarbituric acid reactive substances (TBARS), and activities of superoxide dismutase, catalase and selenium-dependent glutathione peroxidase were measured in rabbits fed a methionine-enriched diet for 6 or 9 mo. Morphological studies of livers were also made. Feeding rabbits the methionine-enriched diet for 9 mo resulted in a significant increase in liver TBARS levels and antioxidant enzyme activities. Moreover, an inflammatory infiltration of portal triads in the treated rabbits were observed. These results indicate that methionine may induce hepatitis by increasing free radical processes.


Archives of Toxicology | 1993

Modulation of procainamide toxicity by selenium-enriched yeast in rats

Michal Toborek; Krystyna Magner-Wróbel; Marian Dróżdż; Alojzy Danch; Ewa Kopieczna-Grzebieniak

Free radical processes are proposed to play a crucial role in the development of procainamide adverse effects. Therefore, selenium, as a potent antioxidant, may modified procainamide toxicity. To test this hypothesis plasma and liver thiobarbituric acid-reacting substances (TBARS), plasma antioxidant activity (AOA), erythrocyte and liver superoxide dismutase (SOD), catalase, as well as selenium-dependent glutathione peroxidase (Se-GPX) were determined in the following four groups of rats: selenium-treated (Se), procainamide-treated (P), procainamide and selenium-treated (P + Se), and control (C). Morphological studies of leukocytes [tested for lupus erythematosus (LE) cells] and liver were also made. Atypical, i.e. enlarged and swollen, leukocytes resulting from procainamide and selenium treatment were observed. These changes were found in four out of five rats in the Se group, eight out of ten in the P group, and in seven out of ten in the P + Se group. LE-like cells were observed in two rats in the P + Se group. A statistically significant decrease in plasma and liver TBARS by 20% and 36%, respectively, increased activity of SOD by 20%, catalase by 48% and Se-GPX by 15% in erythrocytes, and decreased activity of liver SOD by 17% and catalase by 22% were found in the P + Se group as compared to the P group. These results indicated that selenium exerted antioxidant effects on the procainamide-treated rats. However, selenium did not prevent the development of disturbances in leukocyte morphology, on the contrary, it possibly promoted the conversion of leukocytes to LE cells.


Acta Biochimica Polonica | 1990

Concentration of some proteinase inhibitors: alpha 1-antitrypsin and alpha 2-macroglobulin in rabbit blood serum in two models of experimental atherosclerosis.

Rozalia Grabowska-Bochenek; Marian Dróżdż; Ewa Kopieczna-Grzebieniak; Marek Piekarski


Medical Science Monitor | 1998

Effect of radiotherapy and surgery on glyoxalase activities in larynx cancer patient erythrocytes

Danuta Piskorska; Alojzy Danch; Marian Dróżdż; Ewa Kopieczna-Grzebieniak; Grzegorz Namysłowski; Eugeniusz Czecior; Ewa Nowińska


The FASEB Journal | 1997

Aortic oxidative stress in methionine-induced atherosclerosis in rabbits

Michal Toborek; Ewa Kopieczna-Grzebieniak; Marian Dróżdż; Marian Wieczorek


Acta Biochimica Polonica | 1992

Collagenolytic activity in experimental atherosclerosis induced in rabbits fed a cholesterol-rich diet

Grabowska-Bochenek R; Marian Dróżdż; Ewa Kopieczna-Grzebieniak; Mach A; Sobczak A; Wasik T


Revue Roumaine de Biochimie | 1990

Elastin metabolism in different models of experimental atherosclerosis in rabbits. II. Indirect measurement of desmosine biosynthesis in aorta

Ewa Kopieczna-Grzebieniak; Marian Dróżdż; M. Toborek; A. Sobczak; R. Grabowska-Bochenek


Revue Roumaine de Biochimie | 1990

Elastin metabolism in different models of experimental atherosclerosis in rabbits. I. Measurement of elastin degradation level by determination of desmosine concentration in urine

A. Sobczak; Marian Dróżdż; Ewa Kopieczna-Grzebieniak; M. Toborek; R. Grabowska-Bochenek

Collaboration


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Marian Dróżdż

University of Silesia in Katowice

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Michal Toborek

University of Silesia in Katowice

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M. Toborek

University of Silesia in Katowice

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R. Grabowska-Bochenek

University of Silesia in Katowice

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Marian Wieczorek

University of Silesia in Katowice

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A. Sobczak

University of Silesia in Katowice

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Alojzy Danch

Medical University of Silesia

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B. Brandys

University of Silesia in Katowice

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D. Piskorska

University of Silesia in Katowice

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Eugeniusz Czecior

University of Silesia in Katowice

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