Amanda Mahoney

Training pouched rats to find people

Giant African pouched rats equipped with video cameras may be a tenable option for locating living humans trapped under debris from collapsed structures. In the present study, 5 pouched rats were trained to contact human targets in a simulated collapsed building and to return to the release point after hearing a signal to do so. During test sessions, each rat located human targets more often than it located similar-sized inanimate targets on which it had not previously been trained and spent more time within 1 m of the human target than within 1 m of the other targets. Overall, the rats found humans, plastic bags containing clothes, and plastic bags without clothes on 83%, 37%, and 11% of trials, respectively. These findings suggest that using pouched rats to search for survivors in collapsed structures merits further attention.

Pouched rats' detection of tuberculosis in human sputum: comparison to culturing and polymerase chain reaction.

Setting. Tanzania. Objective. To compare microscopy as conducted in direct observation of treatment, short course centers to pouched rats as detectors of Mycobacterium tuberculosis. Design. Ten pouched rats were trained to detect tuberculosis in sputum using operant conditioning techniques. The rats evaluated 910 samples previously evaluated by smear microscopy. All samples were also evaluated through culturing and multiplex polymerase chain reaction was performed on culture growths to classify the bacteria. Results. The patientwise sensitivity of microscopy was 58.0%, and the patient-wise specificity was 97.3%. Used as a group of 10 with a cutoff (defined as the number of rat indications to classify a sample as positive for Mycobacterium tuberculosis) of 1, the rats increased new case detection by 46.8% relative to microscopy alone. The average samplewise sensitivity of the individual rats was 68.4% (range 61.1–73.8%), and the mean specificity was 87.3% (range 84.7–90.3%). Conclusion. These results suggest that pouched rats are a valuable adjunct to, and may be a viable substitute for, sputum smear microscopy as a tuberculosis diagnostic in resource-poor countries.

Tuberculosis detection by giant african pouched rats.

In recent years, operant discrimination training procedures have been used to teach giant African pouched rats to detect tuberculosis (TB) in human sputum samples. This article summarizes how the rats are trained and used operationally, as well as their performance in studies published to date. Available data suggest that pouched rats, which can evaluate many samples quickly, are sufficiently accurate in detecting TB to merit further investigation as a diagnostic tool.

Self-injurious behavior and functional analysis: where are the descriptions of participant protections?

This study examined the reporting of participant protections in studies involving functional analysis and self-injurious behavior and published from 1994 through 2008. Results indicated that session termination criteria were rarely reported and other specific participant safeguards were seldom described. The absence of such information in no way indicates that functional analysis procedures were unethical or otherwise inappropriate, although the fact that participants emitted many self-injurious responses in some studies where no protections were described and no rationale for requiring such a large sample of behavior was provided is cause for concern. Future publications would benefit from the inclusion of clear and detailed specifications of participant protections.

Evaluation of Giant African Pouched Rats for Detection of Pulmonary Tuberculosis in Patients from a High-Endemic Setting.

Background This study established evidence about the diagnostic performance of trained giant African pouched rats for detecting Mycobacterium tuberculosis in sputum of well-characterised patients with presumptive tuberculosis (TB) in a high-burden setting. Methods The TB detection rats were evaluated using sputum samples of patients with presumptive TB enrolled in two prospective cohort studies in Bagamoyo, Tanzania. The patients were characterised by sputum smear microscopy and culture, including subsequent antigen or molecular confirmation of Mycobacterium tuberculosis, and by clinical data at enrolment and for at least 5-months of follow-up to determine the reference standard. Seven trained giant African pouched rats were used for the detection of TB in the sputum samples after shipment to the APOPO project in Morogoro, Tanzania. Results Of 469 eligible patients, 109 (23.2%) were culture-positive for Mycobacterium tuberculosis and 128 (27.3%) were non-TB controls with sustained recovery after 5 months without anti-TB treatment. The HIV prevalence was 46%. The area under the receiver operating characteristic curve of the seven rats for the detection of culture-positive pulmonary tuberculosis was 0.72 (95% CI 0.66–0.78). An optimal threshold could be defined at ≥2 indications by rats in either sample with a corresponding sensitivity of 56.9% (95% CI 47.0–66.3), specificity of 80.5% (95% CI 72.5–86.9), positive and negative predictive value of 71.3% (95% CI 60.6–80.5) and 68.7% (95% CI 60.6–76.0), and an accuracy for TB diagnosis of 69.6%. The diagnostic performance was negatively influenced by low burden of bacilli, and independent of the HIV status. Conclusion Giant African pouched rats have potential for detection of tuberculosis in sputum samples. However, the diagnostic performance characteristics of TB detection rats do not currently meet the requirements for high-priority, rapid sputum-based TB diagnostics as defined by the World Health Organization.

Using Task Clarification and Feedback Training to Improve Staff Performance in an East African Nongovernmental Organization

The present study used a multiple-baseline design to illustrate the effectiveness of an intervention package consisting of a multipurpose job aid and feedback training in improving the performance of supervisors and animal trainers in a nongovernmental organization headquartered in East Africa. Prior to the intervention, the performance of three supervisors and three animal trainers was suboptimal. Performance improved when supervisors were taught to use the job aid and provide feedback, and reached a high and consistent level during a subsequent phase, in which supervisors used the job aid independently. Limited maintenance and generalization data suggest that the intervention package produced lasting and generalized effects, and social validity data suggest that supervisors viewed the intervention as acceptable. These findings appear to be the first experimentally controlled demonstration of the potential value of organizational behavior management in improving performance in nongovernmental organizations working in resource-poor areas.

Using giant African pouched rats to detect human tuberculosis: a review.

Despite its characteristically low sensitivity, sputum smear microscopy remains the standard for diagnosing tuberculosis (TB) in resource-poor countries. In an attempt to develop an alternative or adjunct to microscopy, researchers have recently examined the ability of pouched rats to detect TB-positive human sputum samples and the microbiological variables that affect their detection. Ten published studies, reviewed herein, suggest that the rats are able to detect the specific odor of Mycobacterium tuberculosis, which causes TB, and can substantially increase new-case detections when used for second-line TB screening following microscopy. Further research is needed to ascertain the rats’ ability to detect TB in children and in HIV-positive patients, to detect TB when used for first-line screening, and to be useful in broad-scale applications where cost-effectiveness is a major consideration.

Landmine-detection rats: an evaluation of reinforcement procedures under simulated operational conditions.

Because the location of landmines is initially unknown, it is impossible to arrange differential reinforcement for accurate detection of landmines by pouched rats working on actual minefields. Therefore, provision must be made for maintenance of accurate responses by an alternative reinforcement strategy. The present experiment evaluated a procedure in which a plastic bag containing 2,4,6-trinitrotoluene (TNT), the active ingredient in most landmines, was placed in contact with the ground in a disturbed area, then removed, to establish opportunities for reinforcement. Each of five rats continued to accurately detect landmines when extinction was arranged for landmine-detection responses and detections of TNT-contaminated locations were reinforced under a fixed-ratio 1 schedule. The results of this translational research study suggest that the TNT-contamination procedure is a viable option for arranging reinforcement opportunities for rats engaged in actual landmine-detection activities and the viability of this procedure is currently being evaluated on minefields in Angola and Mozambique.

Ethical Issues and Considerations

Functional behavioral assessment (FBA) refers to a range of methods designed to identify the environmental variables that control problematic behaviors. Methods for collecting these data revealing these variables include indirect measures, such as interviews and questionnaires, or direct methods, such as narrative recording of the antecedents that precede responses of interest and the consequences that follow them. Many behavior analysts believe that the “gold standard” of FBA is experimental functional analysis (FA) (Iwata, Dorsey, Slifer, Bauman, & Richmond, 1982/1994), which systematically arranges consequences for problem behaviors to identify their functions, that is, the reinforcers that maintain those behaviors. FBA is one of several ways of collecting information about clients, and professional organizations such as the American Psychological Association (APA) and the Behavior Analyst Certification Board (BACB) have established general ethical guidelines regarding how assessments should be conducted and interpreted. For example, Standard 9 of the Ethical Principles of Psychologists and Code of Conduct promulgated by the APA (2010) is devoted entirely to assessment. The same is true of Standard 3.0 of the Behavior Analyst Certification Board Guidelines for Responsible Conduct (BACB Guidelines, BACB 2011). That standard is presented in Table 13.1. Any practitioner who abides with the standards established there and elsewhere in the Guidelines is therefore behaving ethically, regardless of whether he or she is involved in functional assessment or another professional activity.

Echoic and self-echoic responses in children.

Eleven typically developing children were assessed on the accuracy of prompted self-echoic responses following a 5-s delay from their initial echoic response, replicating procedures in Esch, Esch, McCart, and Petursdottir (2010) that compared discrepancies between echoic and self-echoic scores of autistic and typically developing children following a 2-s delay. We compared the two studies in terms of age, level tested, and echoic/self-echoic discrepancy scores. Age and test level differences were found to be statistically significant. Results are discussed in terms of discrepant self-echoic performance and selfechoic rehearsal as it relates to participant age, test level, motivating variables, and the development of complex behavior.