Ana Maria Iba Kanashiro

Ocorrência de Salmonella spp. em carcaças de frangos industrialmente processadas, procedentes de explorações industriais do Estado de São Paulo, Brasil

In the last years the demand for safe food has turned into an increasing necessity for market. Besides, the absence of any microorganism responsible for zoonosis is a requirement of national and international rules. However, even with technological development broiler meat is still susceptible to bacteria contamination, especially Salmonella. The aim of this research was to study the occurrence of Salmonella in broiler carcasses, from Sao Paulo slaughter plants ready to be distributed to the market. The research classified the samples according to presence or absence of Salmonella in 25g of the product. One hundred and sixteen frozen broiler carcasses were collected and analyzed, using a conventional culture method, during one year (July 2006 - June 2007). A total of two (1.7%) samples presented Salmonella spp and one (0.8%) Salmonella Enteritidis, numbering three positive samples (2.5%). Although this research showed a lower contamination rate comparing to other studies, the results are still a concern to public health.

Molecular characterization of Brazilian infectious bursal disease virus isolated from 1997 to 2005.

Abstract This retrospective study concerned 41 infectious bursal disease virus (IBDV) isolates obtained from Brazilian broiler and layers flocks by reverse transcription-polymerase chain reaction. Twenty-five of them were identified as very virulent (vv) by restriction enzyme analysis and by further nucleotide and phylogenetic analysis. All of them had the typical amino acid residues, and all clustered in a phylogenetic tree with the vvIBDV strains. Four amino acid substitutions, at positions D213N, G254D, S317R, and D323E, were common to 3 vv isolates, Br/03/DB, Br/03/CK, and Br/04/CR, and differed from other vv isolates and strains. These isolates came from the same locale, but were collected in different years, indicating that the vvIBDVs circulating on Brazilian farms are undergoing slight but continuous exchanges.

Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders

Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (n = 2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemars test (P ≤ .001), and Kappa coefficient showed a weak correlation between the two techniques (k = 0.25; 0.21 ≤ k < 0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS.

Important Aspects of Salmonella in the Poultry Industry and in Public Health

The objective of this review was to discuss relevant issues related to the pathogeny, epidemiology and antimicrobial resistance of Salmonella spp. Due to its economic importance, and because it poses risks to human health, Salmonella spp. is one of the most frequently studied enteropathogens. Nowadays, the disease is considered to be a consequence of interrelated factors, such as food, the environment, vectors, men, utensils and equipments, the production line, animal transit and animal reservoirs.

Ocorrência de Salmonella Spp. em Carcaças de Frango Resfriadas no Período de 2000 A 2010

Ana Lucia Sicchiroli Paschoal Cardoso, Eliana Neire Castiglioni Tessari, Greice Filomena Zanatta Stoppa, Ana Maria Iba Kanashiro, Renato Luis Luciano, Antonio Guilherme Machado de Castro. Ocorrencia de Salmonella Spp. em Carcacas de Frango Resfriadas no Periodo de 2000 A 2010. In: Anais do 12o Congresso Latinoamericano de Microbiologia e Higiene de Alimentos MICROAL 2014 [= Blucher Food Science Proceedings, num.1, vol.1]. Sao Paulo: Editora Blucher, 2014. DOI 10.5151/foodsci-microal-149 Ocorrencia de Salmonella spp. em Carcacas de Frango Resfriadas no Periodo de 2000 A 2010