Andréa Araújo de Vasconcellos

Comparison of methods for quantifying dental wear caused by erosion and abrasion

Various methods have been applied to evaluate the effect of erosion and abrasion. So, the aim of this study was to check the applicability of stylus profilometry (SP), surface hardness (SH) and focus‐variation 3D microscopy (FVM) to the analysis of human enamel and dentin subjected to erosion/abrasion. The samples were randomly allocated into four groups (n = 10): G1‐enamel/erosion, G2‐enamel/erosion plus abrasion, G3‐dentin/erosion, and G4‐dentin/erosion plus abrasion. The specimens were selected by their surface hardness, and they were subjected to cycles of demineralization (Coca‐Cola®‐60 s) and remineralization (artificial saliva‐60 min). For groups G2 and G4, the remineralization procedures were followed by toothbrushing (150 strokes). The above cycle was repeated 3×/day during 5 days. The samples were assessed using SH, SP, and FVM. For each substrate, the groups were compared using an unpaired t‐test, and Pearson correlation coefficients were calculated (α = 5%). For enamel, both profilometry technique showed greater surface loss when the erosion and abrasion processes were combined (P <0.05). The correlation analysis did not reveal any relationships among SH, SP, and FVM to G2 and G4. There were significant correlation coefficients (–0.70 and –0.67) for the comparisons between the FVM and SH methods in enamel and dentin, respectively, in G1 and G3. Choosing the ideal technique for the analysis of erosion depends on the type of dental substrate. SP was not sufficiently sensitive to measure the effects on dentin of erosion or erosion/abrasion. However, SP, FVM and SH were adequate for the detection of tissue loss and demineralization in enamel. Microsc. Res. Tech., 2013.

Environmental pH influences Candida albicans biofilms regarding its structure, virulence and susceptibility to fluconazole.

Candida albicans colonizes sites with different environmental pH. However, it is unclear how these conditions can interfere on biofilms. This study aimed to evaluate the influence of environmental pH on behavior of C. albicans regarding its structure, virulence and susceptibility to fluconazole (FLZ). Minimal inhibitory concentration, minimal fungicidal concentration and time kill were used to evaluate the susceptibility to FLZ in planktonic cells under three pH values (4.0, 5.5, 7.0). These pH values were used for biofilms analysis. C. albicans ATCC 90028 was developed on poly(methlymethacrylate) resin for 48 h. Then, 2.56 μg/mL of FLZ was added to experimental groups for 24 h, and biofilms were analyzed by cell quantification, bioactivity, secretion of proteinases and phospholipases and structure. All data were analyzed by two-way ANOVA, followed by Tukeys test (α = 0.05). For planktonic cells, changes in environmental pH decreased the susceptibility to FLZ. C. albicans biofilms developed at pH 5.5 showed higher cell counts, bioactivity, bio-volume, average thickness and roughness coefficient (p < 0.05). In contrast, the presence of FLZ at pH 4.0 did not influence the structural parameters (p > 0.05), but increased secretion of proteinase and phospholipase (p < 0.05). Within the conditions studied, it was shown that environmental pH modulates the structure, virulence and susceptibility of C. albicans to FLZ.

Candida-Associated Denture Stomatitis: Clinical Relevant Aspects

Candida-associated denture stomatitis is a common fungal infection that affects removable denture wearers. Although Candida spp are considered commensal fungal in the oral cavity, changes in local and/or systemic predisposing factors related to the host conditions may lead to pathogenic form and cause disease. The clinical manifestations are usually associated with the predisposing factors, changing from no symptoms to severe pain and difficulty swallowing. The therapeutic strategies commonly adopted in the clinical practice are the use of topical and/or systemic antifungal, in addition to removing mechanically the plaque from denture surfaces and from underlying mucosa and giving instructions about the correct oral hygiene to the patient. In this context, considering the high prevalence of this disease, a review about the etiology, risk factors, clinical manifestations and therapy management of these patients is of utmost importance.

Influence of cantilever position and implant connection in a zirconia custom implant-supported fixed partial prosthesis: in silico analysis

Introduction: A better tension distribution on implants and abutments in implant-supported fixed partial prosthesis is essential in the rehabilitation of posterior mandible area. Objective: To evaluate the influence of cantilever position and implant connection in a zircônia custom implant-supported fixed partial prosthesis using the 3-D finite element method. Material and method: Four models were made based on tomographic slices of the posterior mandible with a zirconia custom three-fixed screw-retained partial prosthesis. The investigated factors of the in silico study were: cantilever position (mesial or distal) and implant connection (external hexagon or morse taper). 100 N vertical load to premolar and 300 N to molar were used to simulate the occlusal force in each model to evaluate the distribution of stresses in implants, abutments, screws and cortical and cancellous bone. Result: The external hexagon (EH) connection showed higher cortical compression stress when compared to the morse taper (MT). For both connections, the molar cantilever position had the highest cortical compression. The maximum stress peak concentration was located at the cervical bone in contact with the threads of the first implant. The prosthetic and abutment screws associated with the molar cantilevers showed the highest stress concentration, especially with the EH connection. Conclusion: Morse taper implant connetions associated with a mesial cantilever showed a more favorable treatment option for posterior mandible rehabilitation. Descriptors: Dental implant-abutment design; finite element analysis; zirconia. Bastos, Bordin, Vasconcellos et al. Rev Odontol UNESP. 2018 July-Aug.; 47(4): 223-229 224 224/229

Relining effects on the push-out shear bond strength of glass fiber posts

Introduction The correct use of glass fiber posts in endodontically treated teeth is essential for the clinical success of restorative treatment. Objective This study evaluated the push-out shear bond strength of relined (R) or non-relined (NR) glass fiber posts, cemented with self-adhesive resin cement [RelyXTM U100 (U100)] and conventional resin cement [RelyXTM ARC (ARC)]. Material and method Sixty human single-rooted teeth were endodontically treated and divided into ARC-NR; U100-NR; ARC-R; U100-R groups. The teeth were sectioned into cervical, middle and apical thirds, and subjected to the push-out test. Bond strength was analyzed by the Friedman test; cement and post types were compared by the Mann Whitney test. The pattern of failures was evaluated with digital camera through images at 200x magnification, and was classified as adhesive (at the cement/dentin or cement/post interface), cohesive (cement or post), and mixed failures. Result In ARC-NR, bond strength values were higher in the cervical third; in U100-NR and ARC-R they were similar between the thirds. In U100-R, in the cervical and middle thirds the bond strength values were similar, and there was lower value in the apical third. For non-relined glass fiber posts, the highest mean bond strength values were observed with self-adhesive resin cement. Whereas, relined posts cemented with conventional resin cement had stronger cement layer in comparison with non-relined fiber posts. Conclusion The post relining technique was efficient in ARC-R. ARC-NR and U100-R showed improved bond strength in the cervical region of canal walls. The main failures were adhesive at the cement-post interface.

Effect of thermocycling in a hard denture liner and acrylic resin after different chemical and mechanical polishing

Objective: To evaluate the effect of aging in a hard denture reline (New Truliner) and an acrylic resin (Classic) after chemical and mechanical polishing. Methods: Eighty specimens were made, divided randomly between 8 groups: G1.Acrylic Resin Classic (RAC)/Mechanical Polishing (PM); G2.RAC/PM + thermocycling (TR); G3) RAC/Chemical Polishing (PQ); G4.RAC/PQ + TR; G5.New Truliner (NT)/PM; G6.NT/PM + TR; G7. NT/PQ; G8.NT/PQ + TR. The surface hardness was measured before and after polishing, and after TR. The impact resistance tests were measured after all applied treatments. The data were submitted to ANOVA and Tukey test (a=5%). Results: Regardless of the polishing, it was noted that RAC presented significantly higher surface hardness than NT; PM had higher hardness in both materials compared with PQ; on both polishing, the impact resistance of the RAC was higher than NT. Independently of TR test, the impact resistance of the materials that received PQ was higher than received PM. The PQ caused greater changes in properties than the PM. Conclusion: Aging and chemical and mechanical polishing influenced the physical and mechanical properties of hard denture reline and acrylic resin.