Andrea Maria Lazzari

Brucelose canina: relato de caso

The ocurrence of canine brucellosis is described in a kennel from Uruguaiana city. Due to abortion registered at the kennel the suspected disease was brucellosis. The isolation of Bucella canis was realized by culturing the material collected from aborted fetuses, placenta and neonatos. The agar gel immunodiffusion test was used for sorological evaluation and showed that 72.7% (8/11) of the dogs were infected wifh the bacteria. The authors describe also the clinical signs, serological findings and also the isolation of the Brucella canis.

Isolation of Prothoteca zopfii from a case of bovine mastitis in Brazil

The isolation of Prothoteca zopfii, an algae lacking chlorophyll, from bovine mastitic milk, is described herein. The isolation was performed on 8% sheep blood agar, following incubation at 37 °C for 48 h. Based on biochemical tests, susceptibility to clotrimazole, and light and electron microscopic observation of cellular morphology the algae was identified as P. zopfii . The affected animal did not improve following treatment and had to be eliminated.

Pneumonia enzoótica em javalis (Sus scrofa)

The aim of this paper is to describe the clinical, epidemiological, pathological, bacteriological and immunohistochemical aspects of a pneumonia outbreak in a wild pig farm in the Distrito Federal, Brazil. Ninety wild pigs died in a period of five months, and 63 of these had pulmonary lesions. Clinically, the pigs presented reduced growth rate, anorexia, lethargy, cough and dyspnea, especially after they were moved. High body temperature (40oC in average) was verified in some animals. Auscultation revealed moderate pulmonary crepitation and stertors. Pulmonary gross lesions were typical of lobular bronchopneumonia. Lung lesions were characterized by ventral-cranial consolidation in the majority of the cases. The color of affected pulmonary areas varied from diffuse dark red to mosaic pattern (dark red lobule intercalate by grayish lobule) or diffusely grayish. The majority of the lungs had mucopurulent exsudate in the bronchial lumen that also drained from the parenchyma cut surface. Upon microscopy, the changes were characterized by purulent and histiocytic bronchopneumonia with necrotic foci. In some animals, there was BALT hyperplasia associated with perivascular and peribronchial plasma cells and lymphocytes infiltration in most of these cases. Bordetella bronchiseptica and Streptococcus spp. were the most frequently isolated bacteria. Immunohistochemistry evaluation demonstrated Mycoplasma hyopneumoniae on the luminal surface of bronchial and bronchiolar epithelial cells, and the DNA of bacteria was detected by PCR. This is the first report of bronchopneumonia in wild boars associated with M. hyopneumoniae infection.

Aspectos epidemiológicos do Rhodococcus equi em eqüinos do Município de Bagé, RS, Brasil

Epidemiological aspects about Rhodococcus equi (R. equi) at six horse-breeding farms from Bage county, in the state of Rio Grande do Sul, Brazil, were analysed. Soil samples (36) and equine feces (143) were collected. The objective was to verify the presence of R. equi in those samples. Six soil samples were also used for pH and organic matter (OM) determination. R. equi identification was based on equi factor search and morfological, tintorial and biochemistry behavior. All the horse-breeding farms had the R. equi present in soil and feces samples. Soil yielded 75.00% (27/36) positive samples and 66.43% (95/143) of equine feces were positive. The results obtained for pH and OM from the soil had no correlation with the isolation of R. equi from soil samples of the different horse-breeding farms.

Bronchopneumonia in wild boar (Sus scrofa) caused by Rhodococcus equi carrying the VapB type 8 plasmid

BackgroundRhodococcus equi is associated with pyogranulomatous infections, especially in foals, and this bacterium has also emerged as a pathogen for humans, particularly immunocompromised patients. R. equi infections in pigs, wild boar (Sus scrofa) and humans are mainly due to strains carrying the intermediate virulence (VapB) plasmid. In Brazil, R. equi carrying the VapB type 8 plasmid is the most common type recovered from humans co-infected with the human immunodeficiency virus (HIV). R. equi infection in pigs and wild boar is restricted predominantly to the lymphatic system, without any reports of pulmonary manifestations.FindingsThis report describes the microbiological and histopathological findings, and molecular characterization of R. equi in two bronchopneumonia cases in wild boar using PCR and plasmid profile analysis by digestion with restriction endonucleases. The histological findings were suggestive of pyogranulomatous infection, and the plasmid profile of both R. equi isolates enabled the characterization of the strains as VapB type 8.ConclusionsThis is the first report of bronchopneumonia in wild boar due to R. equi. The detection of the VapB type 8 plasmid in R. equi isolates emphasize that wild boar may be a potential source of pathogenic R. equi strains for humans.

AGENTES INFECCIOSOS ISOLADOS DE Chinchilla laniger

This report describes a case of infection for Pseudomonas aeruginosa and outbreaks caused by Bordetella bronchiseptica in chinchila farms from the region of Santa Maria, Southern Brazil. The affected animals presented apathy, anorexia, respiratory impairment and nasal secretion. The isolation of Pseudomonas aeruginosa was carried out through the culture of samples of lung whereas Bordetella bronchiseptica was obtainedfrom lung, liver and swab of traqueal secretion. In this report, the clinical data and the pathological findings as well as the isolation and identification of these agents are described.

Rhodococcus equi: sorologia e avaliação da transferência passiva de anticorpos para potros

Two equine populations were evaluated in relation to serology and passive antibodies against Rhodococcus equi. The first population had 59 foals with no history of mare vaccination against Rhodococcus equi. The foals sera samples and the mare colostrum were examined with the agar gel immunodiffusion test. Antibodies were detected in 32.2% of the foals and also 30.5% (18/59) of colostrum samples examined. However, no passive antibody transfer was observed. The second population, 12 pregnant mares were vaccinated against Rhodococcus equi. Before the vaccination, all mares were seronegative. After vaccination, 91.6% (11/12) of the vaccinated mares were positive on the serologic test. In mare colostrum and foals sera, positive results were 75% (9/12) and 66.7% (8/12) respectively. Passive antibody transmission was observed in the foals and there was a relationship with ingestion of positive colostrum.

Caracterização fenotípica, produção de biofilme e resistência aos antimicrobianos em isolados de Staphylococcus spp. obtidos de casos de mastite em bovinos e bubalinos

The aim of the present study was to evaluate the antimicrobial susceptibility pattern and to perform the phenotypic and genotypic characterization of the Staphylococcus spp. isolates from mastitis cases in cattle (n=30) and buffaloes (n=30). The susceptibility to antimicrobial drugs was performed by disk diffusion test and the presence of efflux pump was evaluated in Mueller Hinton (MH) Agar supplemented with ethidium bromide as well as by detection of msrA gene. Similarly, the PCR technique was done to detect mecA, blaZ and ermA, B e C genes, that were related after with the presence of antimicrobial resistance in disk diffusion test. The formation of biofilm was characterized using Congo Red Agar (CRA), microplate adherence and detection of icaD gene. Staphylococcus spp. isolates shown high antimicrobial susceptibility in disk diffusion test. The multidrug resistance (MDR) rate ranged from 0 and 0,5. In the efflux pump test, 26.7% of Staphylococcus spp. strains were positive in phenotypic method and 6.7% in PCR for msrA gene. The erm, mecA and blaZ genes were detected in 1.7%, 6.7% and 11.7% of Staphylococcus spp. strains respectively. In biofilm production tests, 23.3% of the samples were positive in CRA, 50% in microplate adherence test and 8.3% in icaD gene PCR. The cattle isolates were less sensitive to antimicrobial drugs when compared to the buffaloes ones. The characterization of these isolates is very important to guide a successful antimicrobial therapy. The biofilm presence in the isolates may be associated with other factors besides antimicrobial resistance.