Andreas Fließbach

Long-term effects of organic farming on fungal and bacterial residues in relation to microbial energy metabolism

Samples from the bio-dynamic, bio-organic, and conventional trial, Therwil, Switzerland, were analyzed with the aim of determining the effects of organic land use management on the energy metabolism of the soil microbial biomass and on the fraction of microbial residues. The contents of adenylates, adenosine triphosphate (ATP), glucosamine, muramic acid, and galactosamine were significantly largest in the biodynamic organic farming (BYODIN) treatment and significantly lowest in the conventional farming treatment with inorganic fertilization (CONMIN). In contrast, the ergosterol-to-ATP ratio and fungal C-to-bacterial C ratios were significantly lowest in the BYODIN treatment and significantly largest in the CONMIN treatment. No clear treatment effects were observed for the ergosterol content and the adenylate energy charge (AEC), the ATP-to-microbial biomass C ratio and the ergosterol-to-fungal C ratio. Ergosterol, an indicator for saprotrophic fungal biomass, and fungal residues were significantly correlated. The microbial biomass carbon-to-nitrogen ratio showed a negative relationship with the AEC and strong positive relationships with the ratios ergosterol-to-microbial biomass C, ergosterol-to-ATP and fungal C-to-bacterial C. In conclusion, the long-term application of farmyard manure in combination with organic farming practices led to an increased accumulation of bacterial residues.

Impact of reduced tillage on soil organic carbon and nutrient budgets under organic farming

No-tillage (NT) and reduced tillage (RT) systems are well-known management tools for reducing soil erosion and improving soil fertility. NT and RT may improve the environmental and economic performance of organic farming, but they are still not common practice among organic farmers. This paper presents the effects of tillage [RT versus conventional tillage (CT)], fertilization (slurry versus manure compost) and biodynamic preparations (with versus without) on soil fertility indicators such as soil organic carbon (Corg), microbial biomass and microbial activity, soil nutrients and nutrient budgets in an organic farming system during the first six-year crop rotation period of a long-term experiment on a clayey soil in a temperate climate. RT caused stratification of soil organic carbon (Corg), microbial properties and soil nutrients in the soil profile. Under RT, Corg in the 0–10 cm soil layer increased from 2.19 to 2.61% (w/w) from 2002 to 2008, whereas it remained constant under CT. In both tillage treatments, Corg remained constant in the 10–20 cm soil depth. Microbial biomass C increased by 37% under RT in the 0–10 cm soil depth and microbial activity [dehydrogenase activity (DHA)] was enhanced by 57%. Soil microbial biomass C and DHA in the 10–20 cm soil depth were also higher under RT (+10 and +17%, respectively). Soluble soil P and K were 72 and 40%, respectively, higher in 0–10 cm soil depth under RT when compared with CT. Fertilization showed no effects on the measured soil properties. Biodynamic preparations increased solely the Cmic-to-Nmic (soil microbial biomass C to soil microbial biomass N) ratio by 7% in the 0–10 cm soil depth. Nutrient budgets for P were balanced in all treatments, but N and K exports were higher under RT compared to CT. We conclude that RT is a suitable method for increasing indicators of soil fertility in organic farming systems. The combined effects of RT and an organic farming system with a diverse, ley-based crop rotation and organic fertilization merit further promotion and it may be considered for supporting actions by the agricultural policy schemes.

Estimation by PLFA of Microbial Community Structure Associated with the Rhizosphere of Lygeum spartum and Piptatherum miliaceum Growing in Semiarid Mine Tailings

The objective of this study was to compare the microbial community composition and biomass associated with the rhizosphere of a perennial gramineous species (Lygeum spartum L.) with that of an annual (Piptatherum miliaceum L.), both growing in semiarid mine tailings. We also established their relationship with the contents of potentially toxic metals as well as with indicators of soil quality. The total phospholipid fatty acid (PLFA) amount was significantly higher in the rhizosphere soil of the annual species than in the rhizosphere soil of the perennial species. The fungal/bacterial PLFA ratio was significantly greater in the perennial species compared to the annual species. The fatty acid 16:1ω5c, the fungal/bacterial PLFA ratio and monounsaturated/saturated PLFA ratio were correlated negatively with the soluble contents of toxic metals. The cyc/prec (cy17:0 + cy19:0/16:1ω7 + 18:1ω7) ratio was correlated positively with the soluble contents of Pb, Zn, Al, Ni, Cd, and Cu. The results of the PLFA analysis for profiling microbial communities and their stress status of both the plant species indicate that perennial and annual gramineous species appear equally suitable for use in programmes of revegetation of semiarid mine tailings.

Short- and long-term effects on soil microorganisms of two potato pesticide spraying sequences with either glufosinate or dinoseb as defoliants

Pesticides applied to potatoes in Swiss integrated farming were evaluated with respect to their cumulative effects on soil microorganisms in a study performed under controlled conditions. Potatoes were treated with a series of herbicides, fungicides and insecticides and were finally defoliated either by hand in the untreated control or with the total herbicides Basta (active ingredient glufosinate) or Super Kabrol (active ingredient dinoseb). Twenty-one and 135 days after the last pesticide application soil samples were collected and analysed for microbial biomass, activity and community level substrate utilisation (CLSU). In the short-term, cumulative pesticide side-effects on microbial biomass and microbial activities averaged 19% for the spraying sequence with glufosinate and 45% for the one with dinoseb. After 135 days these values merely returned to normal. Remarkably, only the CLSU patterns based on Biolog ecoplates showed a lasting effect. We consider this an indication of change in microbial catabolic capabilities that may be due either to induced pesticide degradation capabilities or to a change within the microbial community. Even though this method has drawbacks in comparison to molecular methods of microbial community analysis—in particular because of its culture dependence—it gives a first indication that changes in the microbial community may have occurred. The microbial community structure may then be analysed in more detail with molecular tools. As an additional tool to conventional testing of agrochemicals, microbial community analysis may help in the interpretation of pesticide side-effects and open up new possibilities for their observation.

Arthropod food webs in organic and conventional wheat farming systems of an agricultural long-term experiment: a stable isotope approach

1 Agricultural intensification not only alters the structure of arthropod communities, but also may affect biotic interactions by altering the availability of basal resources. We analyzed variations in stable isotope ratios (15N/14N and 13C/12C) of fertilizers, plants, prey and generalist predators in organic and conventional farming systems in a long‐term agricultural experiment [DOK trial (bioDynamic, bioOrganic, Konventionell)]. Two basal resources with pronounced differences in carbon isotope signatures, wheat litter (C3 plant) and maize litter (C4 plant), were used to uncover differences in food web properties between the two farming systems (conventional versus organic). 2 Predators incorporated significantly higher proportions of carbon from wheat sources in organically managed fields, suggesting that they were more closely linked to wheat‐consuming prey in this system. The δ15N values of three predaceous species were more than 2‰ greater in summer than in spring. 3 The results obtained suggest that generalist predators consumed higher proportions of herbivore prey in the organic system and that starvation and intraguild predation rates increased in some predator species with time. 4 Because the effects of farming system and sampling date on predators were species‐specific, conserving a diverse natural enemy community including species with different phenologies and sensitivities to management practices may, in the long term, be a good strategy for maintaining high pest suppression throughout the growing season.

Soil type, management history, and soil amendments influence the development of soil-borne (Rhizoctonia solani, Pythium ultimum) and air-borne (Phytophthora infestans, Hyaloperonospora parasitica) diseases

The impact of soil type, long-term soil management, and short-term fertility input strategies on the suppressiveness of soils against soil-borne (Ocimum basilicum – Rhizoctonia solani, Lepidium sativum – Pythium ultimum) as well as air-borne (Lycopersicon esculentum – Phytophthora infestans, Arabidopsis thaliana – Hyaloperonospora parasitica) diseases was studied. Soils from field trials established in five European sites with contrasting pedo-climatic conditions were examined. Sites included (i) a long-term management field trial comparing organic and conventional farming systems (DOK-trial, Therwil, Switzerland) (ii) a short-term fertility input field trial comparing mineral and organic matter fertilisation regimes (Bonn (BON), Germany) (iii) two short-term fertility input field trials (Stockbridge (STC) and Tadcaster (TAD), UK) comparing the impact of farmyard manure, composted farmyard manure, and chicken manure pellet amendements and (iv) soil from a site used as a reference (Reckenholz (REC), Switzerland). Soil type affected disease suppressiveness of the four pathosystems signficantly, indicating that soils can not only affect the development of soil-borne, but also the resistance of plants to air-borne diseases at relevant levels. Suppressiveness to soil- and air-borne diseases was shown to be affected by soil type, but also by long-term management as well as short-term fertility inputs.

Effects of conventionally bred and Bacillus thuringiensis (Bt) maize varieties on soil microbial biomass and activity

Genetically modified (GM) maize containing genes from the soil bacterium Bacillus thuringiensis (Bt) was cultivated on 29% of the total maize production area worldwide in 2009. Most studies to date compare Bt-maize varieties with their near isogenic lines; however, there is little information on the variability of conventional maize breeding lines and how the effects of Bt varieties are ranked within. In our study on the potential risks of Bt-maize varieties, we analyzed tissue quality and compared the effects of ten conventional and GM maize varieties on soil microbiological properties in a replicated climate chamber experiment. All maize varieties were cultivated twice in the same soil microcosm. Shoot yields and soluble C in leaf tissue of Bt varieties were higher than the ones of non-Bt. Soil dehydrogenase activity was reduced by 5% under Bt varieties compared to non-Bt, while most of the other soil microbial properties (soil microbial biomass, basal respiration) showed no significant differences between Bt and non-Bt varieties. The leaves and roots of one Bt variety were decomposed to a greater extent than the ones of its near isogenic line; the conventional breeding lines also showed higher values. Changes in crop and soil parameters were found when comparing the first and the second crops, but the effects of repeated cropping were the same for all tested varieties. For the studied parameters, the variation among non-Bt-maize varieties was similar to the difference between Bt and non-Bt varieties.

Reply to Leifeld et al.: Enhanced top soil carbon stocks under organic farming is not equated with climate change mitigation

In their letter, Leifeld et al. (1) argue that our metaanalysis to identify differences in soil organic carbon (SOC) between organic (OF) and nonorganic farming [conventional farming (CF)] (2) selected CF systems that were nonrepresentative. This was not the case. We included data from all available pairwise field comparisons between OF and CF identified in the literature. The observed difference in external carbon (C) inputs between OF and CF did not result from a bias in the selection of studies/treatments but was attributable to the fact that the field comparisons we analyzed (2) were not from fertilization experiments but from pairwise farming system comparisons where the design and the underlying treatments reflected the current farming practices in the region in which the studies were conducted at the time the experiments were initiated.

Mineralisation and assimilation processes of 14C-labelled shoots of Stipa capensis in a Negev desert soil

Abstract Stipa capensis, one of the most abundant annual grasses of the experimental area in the central Negev, Israel, was cultivated in a growth chamber under a l4CO2 atmosphere. Shoots of the desert grass were dried, placed in litterbags and fixed on the soil surface or buried to 10 cm depth. 14CO2 mineralisation was followed during the rainy season of 1991/1992, the following summer and the subsequent rainy season. Incorporation of labelled plant carbon by the soil microbial biomass as well as by micro-arthropod and nematode populations was determined in soil samples taken from the vicinity of the litterbags. Immediately after the first rain, soil respiration and 14C mineralisation started, even at soil moisture levels below 5% (−3.75 MPa). Higher 14CO2 evolution occurred with a further increase of soil moisture, although temperature was decreasing. Simultaneously, a high level of incorporation of labelled 14C was observed in all soil biotic compartments under study. The soil microbial biomass was the most active component, assimilating two orders of magnitude more 14C than the soil microarthropods and nematodes together. 14C from the litter moved primarily to the soil space below the litterbag, and was still detectable more than 1 year after the experiment started.

Soil organic matter balances in organic versus conventional farming—modelling in field experiments and regional upscaling for cropland in Germany

The question of whether organic farming leads to higher soil organic matter (SOM) levels in arable soils compared with conventional farming is an ongoing debate. Building on several studies reported in the literature, we hypothesize that the impact on SOM levels is not an intrinsic characteristic of any farming system but is the result of the actual structure of the farming system, in particular, the composition and management of crop rotations, and the availability and utilization of organic manure. The SOM balances for organic versus conventional farming in Germany are compared by considering data on the structure of organic and conventional farming systems from agricultural census reports and then applying the SOM balance model HU-MOD. Preliminary testing confirmed the applicability of the model using a survey on soil organic carbon (SOC) change and SOM balances in four long-term field experiments in Germany and Switzerland and found that more positive SOM balances coincided with higher SOC levels. We therefore conclude that, where the SOM supply level of organic farming systems is higher than in conventional management, a shift from conventional to organic agriculture would increase SOM levels. Upscaling using agricultural census data in Germany, we found that SOM balances of organic farming were more positive than for conventional farming in the scenarios without consideration of animal manure application, but SOM balances for the two systems were not different where animal manure application rates were assumed to be at the current average rate for all cropped land. However, in fact, animal manure availability and application shows strong regional variations, and it is likely that this would affect the mean cropland SOM balance if it were possible to calculate it based on such spatially disaggregated data. We confirm the applicability of simple SOM balance models to compare the impact of farming systems and cropland structures on SOC levels. More work is needed to develop data inputs at a sufficient spatial and structural resolution to support more detailed evaluation.