Andreza Guedes Barbosa Ramos

Antiulcerogenic Activity of the Hydroalcoholic Extract of Leaves of Croton campestris A. St.-Hill in Rodents

Croton campestris A. St.-Hill., popularly known as “velame do campo,” is a species native to the savannah area of Northeast Brazil, which is used by traditional communities in folk medicine for variety of health problems, especially detoxification, inflammation, and gastritis. The hydroalcoholic extract of C. campestris leaves (HELCC) was assessed for its antiulcerogenic effect in gastric lesion models and effect on intestinal motility in mice, and possible mechanisms of action were examined. HELCC showed significant gastroprotective action in all models of gastric ulcer evaluated; the results suggest that this action probably involves the nitric oxide pathway. HELCC did not show alteration of intestinal motility in mice. It was also found that C. campestris represents a promising natural source with important biological potential, justifying some of its uses in folk medicine.

Modulation of antibiotic activity by the hydroalcoholic extract from leaves of Caryocar coriaceum WITTM

Abstract The aim of this study was to identify the presence of tannins, phenols and flavonoids on the hydroalcoholic extract of Caryocar coriaceum leaves (HECCL) and to determine the antioxidant and antibacterial activity of this extract. The extract was tested alone (1024–1 μg/mL) or associated (MIC/8) with several antibiotics in order to identify any antibacterial activity against multiresistant bacterial strains (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa). The existence of tannins, total phenols (901.31 mg/g) and flavonoids (89.68 mg/g) was confirmed in the HECCL. The presence of rutin and quercetin were confirmed by Thin-layer chromatography (TLC). Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, the antioxidant activity of the extract (9 μg/mL) was determined. Moreover, the Minimum Inhibitory Concentration (MIC) value found for HECCL was 1024 μg/mL and the association between HECCL (MIC/8) with benzylpenicillin significantly changed its minimum inhibitory concentration from 2500 to 625 μg/mL against E. coli.

Evidence of insulin-dependent signalling mechanisms produced by Citrus sinensis (L.) Osbeck fruit peel in an insulin resistant diabetic animal model

Citrus sinensis (L.) Osbeck is extensively cultivated worldwide and one of the most consumed fruits in the world. We evaluated the therapeutic properties of the methanol extract from Citrus sinensis fruit peel (CSMe) in high-fat diet-fed streptozotocin-induced insulin-resistant diabetic rats. Body weight, food intake, and water consumption were analysed. Biochemical and molecular biologic indices, and the expression of insulin receptor-induced signalling molecules were assessed to identify possible mechanisms. In addition, we conducted histology of pancreatic and adipose tissues. UHPLC-MS/MS analysis showed the presence of 17 dietary phenolics at substantial concentrations. High-fat diet-fed streptozotocin-induced diabetic rats administered CSMe (50 and 100 mg/kg) had reduced fasting blood glucose (56.1% and 55.7%, respectively) and plasma insulin levels (22.9% and 32.7%, respectively) compared with untreated diabetic control rats. CSMe reversed the biochemical abnormalities in diabetic rats, showed cytoprotective activity, and increased the intensity of the positive immunoreactions for insulin in pancreatic islets. CSMe treatment increased the expression of PPARγ in the adipose tissue and signalling molecules GLUT4 and insulin receptor. Our data suggest that CSMe could optimize glucose uptake of adipose tissues through the insulin-dependent signalling cascade mechanism and it should be investigated in the management of individuals with type 2 diabetes mellitus.

AS EMOÇÕES E OS SENTIMENTOS NA ASSISTÊNCIA DE ENFERMAGEM À CRIANÇA COM CÂNCER

Esta pesquisa surge da necessidade de aprofundar conhecimentos acerca da assistencia dos profissionais de enfermagem a crianca portadora de câncer. O estudo teve como objetivo conhecer aspectos emocionais relacionados a assistencia a crianca com câncer; atraves da pesquisa qualitativa. A coleta de dados foi realizada mediante entrevistas semi-estruturadas e observacao de campo com 14 profissionais da Equipe de Enfermagem do Hospital Infantil Albert Sabin, Fortaleza-Ceara. Os dados foram submetidos a analise de conteudo, sendo identificadas cinco categorias emergentes dos discursos dos sujeitos. Conclui-se que, apesar da sobrecarga emocional a que estao expostos, os profissionais mantem o compromisso na assistencia as criancas, entretanto evidencia-se a necessidade de suporte emocional para assisti-las, pois existe tendencia para exaustao emocional, desânimo e fracasso. Reforca-se a importância da inclusao da disciplina oncologia e do tema morte nos curriculos dos cursos de formacao e a importância da promocao e prevencao em saude no ambiente de trabalho. Palavras chave: Câncer infantil; Assistencia; Sentimentos.

Uso etnofarmacológico de plantas medicinais em infecções geniturinárias por moradoras da Chapada do Araripe, Crato, Ceará – Brasil - doi: 10.5020/18061230.2012.p278

Objective: To determine the ethnopharmacological use of plants in the treatment of urogenital tract infections (UTIs) by women living in the city of Crato, located in Chapada do Araripe, in the interior of the State of Ceara, Brazil. Methods: A cross-sectional study, with a quantitative approach, performed from September to November, 2010, with 25 residents of Chapada do Araripe, who use and/or recommend plants for the treatment of UTIs. Results: The prevalent characteristics of the informants were: age-group between 56 and 60 years old, married, all of them farmers with a monthly income of minimum wage, and most of them with incomplete schooling. Nine plant species were identified as being used by the population against infection, inflammation and pain. The most common forms of usage are the decoction, cooking and the maceration of leaves and barks. Barbatimao and mallow were the most reported species by informants. Conclusion: Popular medicine is greatly adopted by traditional communities and represents an incalculable cultural wealth which should be preserved and evaluated to assure sustainable development. Information brought out by this research may be useful in further pharmacological studies.