Angela Patrícia Santana

Caracterização das lesões por Cysticercus bovis, na inspeção post mortem de bovinos, pelos exames macroscópico, histopatológico e pela reação em cadeia da polimerase (PCR)

Considering the importance of improving methods for diagnosis of bovine Cysticercosis, this study aimed to verify Cysticercus bovis occurrence in different anatomical sites, as head, heart, esophagus, diaphragm, tongue, liver and carcass, examined by federal inspection service. Diagnosis was performed by gross examination, histopatholgy and PCR with boiling DNA extraction for metacestode identification. Of 22043 slaughtered cattle, 713 (3.23%) were infected. The heart was mostly affected with 1.90% (420/22043), followed by head, 1.11% (245/22043), esophagus, 0.08% (18/22043), carcass, 0.07% (15/22043), diaphragm, 0.03% (7/22043), liver, 0.02% (5/22043) and tongue, 0.01% (3/22043). Of the cysts obtained, 58.35% (416/713) were dead and 41.65% (297/713) were alive. The differences among anatomical sites and cysts status were significant (p<0.05). Of the 416 dead cysts 253, characterized by nodular firm whitish lesions, containing yellowish material, some times in calcareous aspect were examined for histopathology. The histological exams of these cysts yielded granulomatous lesions, whose centers were characterized by caseous and/or calcareous material, multinucleate giant cells, histiocytes in palisade and infiltrate composed predominantly by lymphoid cells, wrapped up by fibrosis. Some times the lesions peripheries had granulation tissue and mineralized areas, like linear blade. The parasite debris were like a hyaline, non cellular material with spherical and ovoid, basophilic, eosinophilic and colorless corpuscles. These corpuscles were seen rarely, some times, among inflammatory reaction. Fibrous nodules, rich in lymphoid or mixed infiltrates, were frequently seen. Of the live cysts subjected to PCR with boiling DNA extraction, 65% (13/20) were positive for C. bovis, confirming the ambulatory diagnosis and the efficacy of the PCR procedure used. Due to microscopic and PCR diagnostic exams of C. bovis, mainly in the liver and esophagus, it is suggested changes in the 176 article of the regulatory inspection, by including these sites in the bovine routine inspection at the slaughterhouses.

Doságem de cortisol sánguíneo em suínos submetidos áo mánejo pré-ábáte e insensibilizáção elétricá

SUMMARY The dosage of cortisol concentration has beenused as stress measure parameter of swinebreedings. Serum cortisol concentration wasdetermined in finishing pigs in rest (group 1) andin finishing pigs submmited to pre-slaughter han-dling and electric sttuning (group 2). Blood fromslaughtered swine were collected immediatelyafter sttuning. Cortisol concetration average forgroup 1 animal was of 21.70 ng/ml while for thegroup 2 was of 74.5 ng/ml, the difference betweencortisol levels were significant (p<0.001)suggesting stressfull condition of pre-slaughterhandilng and electric sttunig for those studied. ThepH average of carcasses from the animalsslaughtered after 24 h in the cold chamber was 6.2and in their visual evaluation, carcasses withcharacteristics PSE (pale, soft and exsudative) orDFD (dry, firm and dark) were not detected. INTRODUCAOA implementacao de nova tecnologianos sistemas de criacao, o melhoramentogenetico do rebanho, o controle sanitario,vem consolidando o consumo da carnesuina seja industrializada, ou

Desempenho de suínos de dois cruzamentos de linhagens comerciais criados em cama sobreposta

Este experimento foi conduzido para avaliar o desempenho de suinos criados em cama sobreposta, foram utilizados 480 animais, distribuidos em delineamento de blocos ao acaso, num arranjo fatorial 2 x 2 (cruzamentos x sexos), com tres repeticoes de quarenta animais por unidade experimental. Foi avaliada a espessura de toucinho (ET) entre a 10a e 11a costela e na altura P2 e area de olho de lombo (AOL) utilizando-se ultra-som em tempo real e as caracteristicas de desempenho. Verificou-se que houve efeito do sexo, onde machos apresentaram resultados superiores para consumo semanal (CS) e diario (CD) de racao, entretanto, a conversao (CA) e eficiencia alimentar (EA) apresentaram resultados inferiores quando comparados as femeas. Nao se observou efeito no ganho de peso diario (GPD) e peso final (PF), entre macho e femea. Suinos provenientes de ambos os cruzamentos e sexos apresentaram resultados satisfatorios e podem ser produzidos no sistema de cama sobreposta, entretanto, as femeas suinas apresentaram melhor desempenho zootecnico.

Ocorrência e diferenciação de espécies de Listeria spp. em salsichas tipo hot dog a granel e em amostras de carne moída bovina comercializadas no Distrito Federal

Listeria monocytogenes is a pathogen disclosed by foods. Its accurate identification is important for the correct determination of the risk associated with the ingestion of food. The aim of this research was to determine the occurrence of Listeria spp. in samples of hot dog sausages and ground beef, sold in bulk in the Federal District. A total of 162 samples, 127 hot dog sausages and 35 samples of ground beef cattle, were analyzed. The isolation and identification of the genus were made by conventional methodology and distinction of species was verified by specific biochemical kit (API-Listeria®) and by restriction fragment length polymorphism (PCR-RFLP) of 23S rRNA gene. Twenty-six strains of Listeria spp. were isolated. Samples of hot dog sausages identified 18 strains of Listeria innocua and 08 strains of Listeria monocytogenes. Of the 35 samples of ground beef cattle were isolated, 16 strains of Listeria spp., 12 strains of Listeria innocua and 04 strains of Listeria monocytogenes. There was total agreement in the distinction of Listeria species using the two methods. The presence of Listeria spp. in samples of hot dog sausages and ground beef, sold in bulk in commercial establishment of the Federal District represents a risk to consumer health.

Avaliação da qualidade microbiológica de queijos do tipo Minas padrão de produção industrial, artesanal e informal

This study aimed at evaluating the microbiological quality of Minas type cheeses, manufactured in industrial scale under federal inspection, artisanal production (from unpasteurized milk) under state and municipal inspections and informal (home production). Also the Minas type cheeses consumption habits among the population of Distrito Federal, Brazil were assessed. Microbiological quality was evaluated in 21 samples by performing the following analyses: coliforms counts at 30 °C and 45 °C, enumeration of coagulasepositive Staphylococcus and detection of Listeria monocytogenes and Salmonella spp. According to the RDC 12/2001 from ANVISA, 57.14 % of industrial, 100 % of informal and 100 % of artisanal samples were noncompliant in the coagulase-positive Staphylococcus counts. In addition, 71.42 % of informal samples and 14.28 % of artisanal and informal cheeses showed coliforms counts at 45 oC above the permitted one. No sample was positive for L. monocytogenes and Salmonella spp. A simplified questionnaire regarding Minas cheese consumption was applied to 50 persons at purchase time, and 47 % of the interviewees preferred the taste of informal cheeses. Nevertheless, the informal cheeses are not inspected and do not follow the production standards; therefore they represent a risk to the public health.