Anja Boisen

High throughput label-free platform for statistical bio-molecular sensing.

Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

Simulation and Measurement of Angle Resolved Reflectance from Black Si Surfaces

In this work angle-resolved reflectance from nanostructured Si surfaces realized by maskless RIE texturing has been simulated and measured. The simulation and experimental measurement data show the same trend. Experimentally a total reflectance below 1% for incident angles below 30 and specular reflectance below 0.1% at incident angles below 70 is seen. In both simulation and experiment the specular reflectance is below 10% at incident angles below 65 and below 1% at incident angles below 45 in the case of non-linear graded refractive index. From the simulation results the non-linear graded refractive index yields lower reflectance than the linearly graded refractive index.