Ann Cowling

Visual control of flight speed in honeybees

SUMMARY Visual control of flight speed in honeybees (Apis mellifera L.) was investigated by training them to fly through a specially constructed tunnel in which the motion, contrast and texture of the patterns lining the walls could be varied. Manipulation of pattern motion revealed that the speed of flight is controlled by regulating the image motion that is experienced by the eyes. Flight speed is surprisingly robust to changes in the contrast and/or spatial texture of the visual environment, suggesting that the underlying movement-detecting mechanisms estimate the speed of image motion in the eye largely independently of these parameters. This ensures that flight speed depends primarily on the distances to nearby surfaces and not upon their particular visual properties, such as contrast or visual texture. The removal of image motion cues drastically compromises the regulation of flight speed, underscoring their role in this function.

Eucalyptus foliar chemistry explains selective feeding by koalas.

The koala is the quintessential specialist herbivore, feeding almost exclusively on Eucalyptus foliage. Consequently, the limitations imposed on the koalas diet by plant defences indicate the extent to which evolutionary adaptations allow mammalian herbivores to circumvent such defences. We tested whether a recently discovered group of plant secondary metabolites, the formylated phloroglucinol compounds (FPCs), deters koalas from feeding on some eucalypt foliage. We found that captive koalas ate less foliage in a single night from trees with high FPC concentrations. Individual trees also differ in the types of FPC they possess, but for a given eucalypt species, most FPCs were similarly effective deterrents. Two closely related and sympatric eucalypt species could be clearly separated by the amounts that koalas ate from each; however, this difference could not be explained by total FPC concentrations alone. We suggest, that in this case, the presence of a distinct type of FPC deters koala herbivory on the less palatable species, and may have facilitated the evolutionary divergence of these species. We conclude that plant defences probably play an important role in determining the distribution and abundance of koalas.

Differential susceptibility to Eucalyptus secondary compounds explains feeding by the common ringtail (Pseudocheirus peregrinus) and common brushtail possum (Trichosurus vulpecula)

The effect of two plant secondary metabolites, tannins and formylated phloroglucinol compounds (FPCs), on the intake of Eucalyptus foliage by common ringtail (Pseudocheirus peregrinus) and common brushtail possums (Trichosurus vulpecula) was studied. We manipulated the amount of tannin that was free to bind with protein by coating foliage with polyethylene glycol 4000 (PEG) and relied on natural intraspecific variation in FPC concentrations. In contrast to ringtail possums, brushtail possums showed a greater tolerance to FPCs and ate more foliage when it was coated with PEG, suggesting that tannins limited their food intake. Brushtails detected the effects of tannins through immediate oral sensations rather than through systemic effects. Ringtail possums appeared highly tolerant of foliar tannins yet susceptible to low concentrations of FPCs. We could not detect any interaction between tannins and FPCs that affected the intake of Eucalyptus foliage by either species of possum. Although ringtail and brushtail possums are widely regarded as specialist and generalist folivores, respectively, their differential susceptibility to co-occurring secondary metabolites suggests greater complexity. Each possum species appears to be a specialist in its own right, which leads to a partitioning of available foliage. Brushtails avoid tannins and ringtails avoid FPCs.

Visual control of flight speed and height in the honeybee

The properties of visually guided flight speed and height control were investigated by training honeybees (Apis mellifera L.) to fly through a tunnel in which the visual cues in the lateral and ventral visual fields could be varied by changing the patterns on the walls and floor of the tunnel The results show that honeybees regulate their flight speed by keeping the velocity of the image of the environment in their eye constant The results also show that honeybees use visual information from the ground to control their height above the ground The findings of this study reveal that the mechanisms of flight speed and height control in the honeybee are perfectly adapted for extracting information from a complex visual environment using simple sensors and computations Consequently, the techniques of visual guidance that are reported here suggest insect-inspired strategies for the control of aircraft flight.

Leaf shape linked to photosynthetic rates and temperature optima in South African Pelargonium species

The thermal response of gas exchange varies among plant species and with growth conditions. Plants from hot dry climates generally reach maximal photosynthetic rates at higher temperatures than species from temperate climates. Likewise, species in these environments are predicted to have small leaves with more-dissected shapes. We compared eight species of Pelargonium (Geraniaceae) selected as phylogenetically independent contrasts on leaf shape to determine whether: (1) the species showed plasticity in thermal response of gas exchange when grown under different water and temperature regimes, (2) there were differences among more- and less-dissected leafed species in trait means or plasticity, and (3) whether climatic variables were correlated with the responses. We found that a higher growth temperature led to higher optimal photosynthetic temperatures, at a cost to photosynthetic capacity. Optimal temperatures for photosynthesis were greater than the highest growth temperature regime. Stomatal conductance responded to growth water regime but not growth temperature, whereas transpiration increased and water use efficiency (WUE) decreased at the higher growth temperature. Strikingly, species with more-dissected leaves had higher rates of carbon gain and water loss for a given growth condition than those with less-dissected leaves. Species from lower latitudes and lower rainfall tended to have higher photosynthetic maxima and conductance, but leaf dissection did not correlate with climatic variables. Our results suggest that the combination of dissected leaves, higher photosynthetic rates, and relatively low WUE may have evolved as a strategy to optimize water delivery and carbon gain during short-lived periods of high soil moisture. Higher thermal optima, in conjunction with leaf dissection, may reflect selection pressure to protect photosynthetic machinery against excessive leaf temperatures when stomata close in response to water stress.

Factors affecting the detection of possums by spotlighting in Western Australia

This paper describes how environmental factors, survey method procedures and differences in forest structure resulting from logging relate to the detection of koomal (common brushtail possum, Trichosurus vulpecula hypoleucus) and ngwayir (western ringtail possum, Pseudocheirus occidentalis). A total of 169 vehicle-based spotlight surveys of possums within native jarrah (Eucalyptus marginata) forest was conducted on three transects over eight years (1996–2003). Up to 5.7 koomal and up to 3.3 ngwayir were detected per kilometre per transect side. Only one ngwayir was detected during the eight surveys conducted between 2001 and 2003. More koomal were seen in spring and autumn (i.e. September–November and March–May respectively) and more ngwayir were seen between October and April. Although surveys were not conducted on very rainy or excessively windy nights, fewer possums were nonetheless seen on nights following rainy days and on cold nights. Cloud cover also affected sightings of koomal. The time taken to complete the surveys increased in conjunction with the number of possums detected, on account of the time required to record data. The importance of standardising travelling speed also is emphasised. Possum spotlight counts differed between recently logged and unlogged areas. However, these findings were not supported by complementary koomal abundance estimates derived from trapping, suggesting that vegetation structure may affect detectability. Factors such as the lunar cycle, wind speed and survey start time after sunset did not significantly affect detection rates of either species. On the basis of these findings, specific survey conditions can be selected to improve spotlight detection efficiency.

Habitat models for the four-fingered skink (Carlia tetradactyla) at the microhabitat and landscape scale

A spatially nested experimental design was used to survey reptiles in a sheep- and cattle-grazing landscape in southern New South Wales, Australia. Sixteen landscape units were chosen on the basis of their aspect, topography and amount of tree cover. Across these landscape units, 288 pitfall traps were established at 144 plots arranged in 48 sites. Each plot was searched for reptiles and pitfall-trapped in two separate survey periods. The four-fingered skink (Carlia tetradactyla) was the most commonly encountered reptile species, and detailed statistical habitat models were constructed for this species. The four-fingered skink responded to both landscape scale and microhabitat variables. The species was more likely to be detected in landscape units characterised by a high amount of tree cover. Adults were most likely to inhabit microhabitats dominated by box- or gum-type eucalypts, with a moderate amount of canopy cover and with a large number of spiders. Juveniles were most likely to inhabit microhabitats with a moderate amount of weed invasion where shrubs were present. Finally, the four-fingered skink was more likely to be found at sites with at least two other co-occurring small reptile species. Body condition and tail loss of adult skinks were not related to the habitat or microhabitat where a given individual was found. The results are discussed in relation to current herpetological research, and some long-term conservation implications are highlighted.

Bird populations in native forest patches in south-eastern Australia: the roles of patch width, matrix type (age) and matrix use

We surveyed birds in patches of native eucalypt forest and in surrounding exotic matrix (Radiata pine forests) in south-eastern Australia. Our objectives were: (1) to examine the influence of the width of native forest patches and the age of surrounding pine forests on bird occurrence in patches of native forest; and (2) to verify the relationship between the use of the surrounding pine matrix and bird species response to variation in width of patches of native forests. A total of 32 study sites (boundaries between eucalypt and pine forests) were surveyed. Birds were counted by the area search method within 0.5-ha quadrats. Data were analysed using generalised linear models. Wide patches of eucalypt forest supported higher species richness and greater numbers of birds, such as foliage searchers and nectarivores, than narrow patches. Matrix age also influenced the occurrence of some species in native patches. The abundance of species in wide and narrow patches of native forest was related to their use of the matrix. This was true for native forests surrounded by old but not by young pine forests. We suggest that management in wood production landscapes take into account both characteristics of native patches and the surrounding matrix. Negative impacts of fragmentation in managed landscapes might be reduced by promoting matrix types that are suitable for bird species.

A comparison of survey methods for arboreal possums in jarrah forest, Western Australia

Comparative trials of different survey methods were conducted in the southern jarrah (Eucalyptus marginata) forest to determine the most efficient means of detecting koomal (common brushtail possum, Trichosurus vulpecula hypoleucus) and ngwayir (western ringtail possum, Pseudocheirus occidentalis). In particular, we examined different trapping and spotlighting methods and compared these with scat surveys. Six different trapping methods (derived by combining three bait types and two trap positions) were compared at six sites. Significantly fewer koomal were caught on ‘universal’ bait (i.e. peanut butter, rolled oats and sardines) than on flour-based baits using rose oil or Eucalyptus oil as lures. Significantly more individuals of both possum species were caught in arboreal traps than in ground traps (P < 0.001 in both cases). Recapture rates of koomal were high, whereas ngwayir were rarely retrapped. There were no detection differences between six different spotlighting methods (derived by combining three spotlight intensities with two filter colours) for koomal. Significantly more ngwayir were detected using 50-W or 100-W lights than 20-W lights (P = 0.01). There were no significant differences in the detection rates for ngwayir using red or white light. There were, however, significant observer differences in the number of possums of both species detected (koomal, P = 0.025; ngwayir, P = 0.004). Spotlighting detected, on average, only 4.9% of the koomal ‘known to be alive’ by trapping. However, spotlighting with a 50-W or 100-W spotlight detected more ngwayir than did trapping. Koomal abundance measures derived from scat surveys were not related to trapping or spotlight abundance estimates. For ngwayir, however, scat counts were strongly related to spotlight counts and there were no significant observer differences for the former. We conclude that koomal are more effectively surveyed using arboreal trapping with rose or Eucalyptus lures. Ngwayir are best surveyed using scat surveys or 50-W spotlights.

Life-history phenotypes in a live-bearing fish Brachyrhaphis episcopi living under different predator regimes: seasonal effects?

Several key life-history attributes in a tropical live-bearing fish, Brachyrhaphis episcopi, have previously been shown to differ between populations that co-occur with large predatory fish (Characin sites) and those that do not (Rivulus sites). Here we show that differences between Characin and Rivulus localities are also repeatable over time; patterns observed in the wet season also persisted during the dry. Both sexes reached maturity at a smaller size at Characin sites. Although there was no difference in fecundity between larger females living in different predator communities, smaller females at Characin sites produced more offspring. Females also produced smaller offspring at Characin localities. These differences are remarkably similar to those reported in two other species of live-bearing fish, B. rhabdophora and Poecilia reticulata suggesting possible convergent adaptation in life-history strategies due to predator-mediated effects or correlates thereof. We also found seasonal changes in life-history traits that were independent of predator community. In the wet season, mature males were larger, females allocated more to reproduction, and offspring mass was also greater. The results of our study generate testable predictions using B. episcopi to further our understanding of life-history evolution.