Anthony J. Brake
Chiron Corporation
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Publication
Featured researches published by Anthony J. Brake.
Cell | 1984
David Julius; Anthony J. Brake; Lindley Blair; Riyo Kunisawa; Jeremy Thorner
S. cerevisiae kex2 mutants are defective for the production of two biologically active secreted peptides: killer toxin and the mating pheromone, alpha-factor. Both molecules are excised from larger precursor polypeptides. In normal cells, the alpha-factor precursor is core-glycosylated and proteolytically processed intracellularly. In kex2 mutants, however, prepro-alpha-factor is not proteolytically cleaved and is secreted in a highly glycosylated form. All kex2 mutants examined (three independent alleles) lack a Zn++-sensitive membrane-associated endopeptidase with specificity for cleaving on the carboxyl side of a pair of basic residues. Absence of this activity cosegregates with the other phenotypes of a kex2 lesion in genetic crosses. The normal KEX2 gene was isolated by complementation of three of the phenotypes conferred by the kex2-1 mutation. The cloned DNA, either on a multicopy plasmid or integrated into the genome, restores both enzymatic activity in vitro and the normal pattern of proteolytic processing and glycosylation of prepro-alpha-factor in vivo. Gene dosage effects suggest that KEX2 is the structural gene for the endopeptidase.
Nature Biotechnology | 1990
J.A. van den Berg; K.J. van der Laken; A. van Ooyen; T.C.H.M. Renniers; Krijn Rietveld; A. Schaap; Anthony J. Brake; R.J. Bishop; K. Schultz; D. Moyer
Archive | 1987
Anthony J. Brake
Archive | 1987
Anthony J. Brake; Johan A. Van den Berg
Archive | 1983
Anthony J. Brake
Archive | 1984
Anthony J. Brake; Lawrence S. Cousens; Mickey S. Urdea; Pablo Valenzuela
Archive | 1988
Pablo Valenzuela; Anthony J. Brake; Anne Randolph
Archive | 1986
Anthony J. Brake; Lawrence S. Cousens; Mickey S. Urdea; Pablo Valenzuela
Archive | 1985
Philip J. Barr; Steven Rosenberg; Anthony J. Brake
Archive | 1984
Philip J. Barr; Anthony J. Brake; Guy Mullenbach