Antonella Spagnoletti
University of Ferrara
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Featured researches published by Antonella Spagnoletti.
Natural Product Research | 2015
Massimo Tacchini; Antonella Spagnoletti; Matteo Marieschi; Augusta Caligiani; Renato Bruni; Thomas Efferth; Gianni Sacchetti; Alessandra Guerrini
Decoctions (DECs) and hydro-alcoholic extracts (HEs) prepared from roots of Boerhaavia diffusa L. (Nyctaginaceae) and Curculigo orchioides Gaertn. (Hypoxidaceae) were phytochemically characterised by HPLC-DAD and profiled for their antioxidant, antigenotoxic and cytotoxic activities. B. diffusa DEC was rich in ferulic acid and vanillin, while the HE also contained boeravinone B and eupalitin. Both C. orchioides HE and DEC displayed the main occurrence of orcinol-β-d-glucoside and curculigoside A. Antioxidant activity was assayed through spectrophotometric DPPH, ABTS and β-carotene bleaching test, and using (HP)TLC bioautographic strategies. For both crude drugs, HE was the best performing preparation. Properly modified SOS-Chromotest evidenced a 10% inhibition by phytocomplexes against 4-nitroquinoline-N-oxide, and a higher bioactivity for vanillin (36.60 ± 1.68%) and ferulic acid (35.09 ± 1.53%). C. orchioides HE was the preparation which showed higher cytotoxicity against drug-sensitive human T-lymphoblastoid cell line (CCRF-CEM) and multidrug-resistant leukaemia cell line (CEM/ADR5000), and eupalitin was the only pure compound to exhibit an IC50 value.
Evidence-based Complementary and Alternative Medicine | 2016
Alessandra Guerrini; Gianni Sacchetti; Alessandro Grandini; Antonella Spagnoletti; Mercedes Asanza; Laura Scalvenzi
Bioautography has been used as rapid and easy strategy to detect and identify bioactive fractions/molecules in the never before investigated Hedyosmum sprucei Solms (Chloranthaceae) essential oil (EO). The antioxidant activity, performed through DPPH bioautographic assay and spectrophotometric evaluations (IC50 = 230 ± 10 µg/mL), seemed to be mainly due to α-cadinol and α-muurolol. (HP)TLC bioautography, focused on antimicrobial capacities, pointed out α-cadinol, α-muurolol, τ-muurolol, caryophyllene oxide, and methyleugenol as the most effective compounds against Staphylococcus aureus, considered as testing strain. Moreover, the microdilution method, assessed among a wide panel of microorganisms, revealed Listeria grayi and Staphylococcus aureus as the most sensitive among human tested strains and Clavibacter michiganensis among phytopathogens. GC-MS chemical profile showed that bioactive molecules represented only a small quantity of the whole EO: germacrene D (23.16%), β-caryophyllene (15.53%), δ-cadinene (5.50%), α-copaene (5.08%), and α-phellandrene (3.48%) were the main compounds, highlighting an uncommon composition among the genus Hedyosmum. Finally, H. sprucei EO was checked for cytotoxic potential against A549 (lung cancer) and MCF-7 (breast cancer) cell lines showing promising cytotoxic effects against both cell lines after 48 h (IC50 A549 = 44.05 ± 2.35 µg/mL; IC50 MCF-7 = 32.76 ± 4.92 µg/mL) and 72 h (IC50 A549 = 43.55 ± 2.80 µg/mL; IC50 MCF-7 = 33.64 ± 0.43 µg/mL).
Plant Biosystems | 2018
Roberta Tardugno; Antonella Spagnoletti; Alessandro Grandini; I. Maresca; Gianni Sacchetti; Federica Pellati; Stefania Benvenuti
Abstract Cedrelopsis grevei H. Baillon bark, endemic plant from Madagascar, is used in folk medicine for the treatment of rheumatism, muscular pain, and for its antifungal and antibiotic activities. In this paper, the phytochemical composition, antioxidant, antimicrobial, and cytotoxic activities of C. grevei bark essential oil (EO), its non-polar (I and II) and polar (III) fractions and its main compounds (ishwarane, β-elemene and α-copaene) were investigated. The GC–MS analysis pointed out the presence of 36 components, representing about 80% as semi-quantitative characterization of the total. The presence of ishwarane, β-elemene and α-copaene as the main constituents highlighted its peculiar composition as a sesquiterpene-rich phytocomplex. Moreover, the quantification was performed for the first time by means of the experimental and predicted response factors (ERFs and PRFs, respectively). As regards the biological activity, C. grevei EO and its fractions showed weak antioxidant activity against Trolox. The whole EO demonstrated instead considerable antimicrobial activity against Staphylococcus aureus and Staphylococcus epidermidis, while its polar-fraction evidenced an interesting bioactivity against Pseudomonas aeruginosa and Candida albicans. Finally, C. grevei EO and its fractions exhibited an interesting cytotoxic activity on human lung cancer cells (A549) and human colorectal cancer cells (CaCo-2).
Molecules | 2017
Laura Scalvenzi; Alessandro Grandini; Antonella Spagnoletti; Massimo Tacchini; David Neill; José Luis Ballesteros; Gianni Sacchetti; Alessandra Guerrini
In this study, we performed the chemical characterization of Myrcia splendens (Sw.) DC. (Myrtaceae) essential oil from Amazonian Ecuador and the assessment of its bioactivity in terms of cytotoxic, antibacterial, and antioxidant activity as starting point for possible applicative uses. M. splendens essential oil, obtained by hydro-distillation, was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Flame Ionization Detector (GC-FID): the major components were found to be trans-nerolidol (67.81%) and α-bisabolol (17.51%). Furthermore, we assessed the cytotoxic activity against MCF-7 (breast), A549 (lung) human tumor cell lines, and HaCaT (human keratinocytes) non-tumor cell line through 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test: promising results in terms of selectivity and efficacy against the MCF-7 cell line (IC50 of 5.59 ± 0.13 μg/mL at 48 h) were obtained, mainly due to α-bisabolol. Furthermore, antibacterial activity against Gram positive and negative bacteria were performed through High Performance Thin Layer Chromatography (HPTLC) bioautographic assay and microdilution method: trans-nerolidol and β-cedren-9-one were the main molecules responsible for the low antibacterial effects against human pathogens. Nevertheless, interesting values of Minimum Inhibitory Concentration (MIC) were noticeable against phytopathogen strains. Radical scavenging activity performed by HPTLC bioautographic and spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) approaches were negligible. In conclusion, the essential oil revealed a good potential for plant defense and anti-cancer applications.
Phytotherapy Research | 2015
Giancarlo A. Statti; Mariangela Marrelli; Filomena Conforti; Antonella Spagnoletti; Massimo Tacchini; Carmela Fimognari; Eleonora Brognara; Roberto Gambari; Gianni Sacchetti; Alessandra Guerrini
Indian Sarsaparilla (Hemidesmus indicus R. Br.) is widely used in Indian traditional medicine. In the present work, we explored the effects of decoction, traditional Ayurvedic preparation, and hydroalcoholic extract, a phytocomplex more traditionally studied and commercialized as food supplement in western medicine, from the roots as possible source of chemicals with new functional potential linked to their nutritional uses. The antiproliferative and antioxidant properties were assayed. To test antiproliferative affects, different cancer cell lines, growing both as monolayers (CaCo2, MCF‐7, A549, K562, MDA‐MB‐231, Jurkat, HepG2, and LoVo) and in suspension (K562 and Jurkat) were used. The decoction showed strong activity on HepG2 cells, while the hydroalcoholic extracts were active on HepG2, LoVo, MCF‐7, K562, and Jurkat cell lines. Weak inhibition of cancer cell proliferation was observed for the principal constituents of the preparations: 2‐hydroxy‐4‐methoxybenzaldehyde, 2‐hydroxy‐4‐methoxybenzoic acid, and 3‐hydroxy‐4‐methoxybenzaldehyde that were tested alone. The antiradical activity was tested with 2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid)diammonium salt tests and inhibition of nitric oxide production in lipopolysaccharide‐stimulated RAW 264.7 macrophages. Interesting result has also been obtained for hydroalcoholic extract regarding genoprotective potential (58.79% of inhibition at 37.5 µg/mL). Copyright
Natural Product Communications | 2016
Antonella Spagnoletti; Alessandra Guerrini; Massimo Tacchini; Vittorio Vinciguerra; Claudia Leone; Immacolata Maresca; Giovanna Simonetti; Gianni Sacchetti; Letizia Angiolella
Journal of Cosmetics, Dermatological Sciences and Applications | 2014
Gabriela Sprada Tavares da Mota; Angela Bonjorno Arantes; Gianni Sacchetti; Antonella Spagnoletti; Paola Ziosi; Emanuela Scalambra; Silvia Vertuani; Stefano Manfredini
Journal of applied botany and food quality | 2014
Alessandra Guerrini; Damiano Rossi; Alessandro Grandini; Laura Scalvenzi; Paco Fernando Noriega Rivera; Elisa Andreotti; Massimo Tacchini; Antonella Spagnoletti; Irene Poppi; Silvia Maietti; Gianni Sacchetti
Valorizzazione della biodiversità italiana: le proprietà salutistiche delle piante alimentari | 2015
Massimo Tacchini; Antonella Spagnoletti; Alessandro Grandini; Thomas Efferth; Gianni Sacchetti; Alessandra Guerrini
Vorbild Natur | 2014
Massimo Tacchini; Antonella Spagnoletti; Eleonora Brognara; Roberto Gambari; Filomena Conforti; Mariangela Marrelli; Alessandra Guerrini; Thomas Efferth; Gianni Sacchetti