Antonio Montagnoli

Fine-root seasonal pattern, production and turnover rate of European beech (Fagus sylvatica L.) stands in Italy Prealps: Possible implications of coppice conversion to high forest

Abstract The aim of this study was to investigate the possible effects of coppice conversion to high forest on the beech fine-root systems. We compared the seasonal pattern of live and dead fine-root mass (d < 2 mm), production and turnover in three beech stands that differed in management practices. Tree density was higher in the 40-year-old coppice stand than in the stands that were converted from coppice to high forest in 1994 and 2004, respectively. We found that a reduction in tree density reduced the total fine-root biomass (Coppice stand, 353.8 g m−2; Conversion 1994 stand, 203.6 g m−2; Conversion 2004 stand, 176.2 g m−2) which continued to be characterised by a bimodal pattern with two major peaks, one in spring and one in early fall. Conversion to high forest may also affect the fine-root soil depth distribution. Both fine-root production and turnover rate were sensitive to management practices. They were lower in the Coppice stand (production 131.5 g m−2 year−1; turnover rate 0.41 year−1) than in the converted stands (1994 Conversion stand: production 232 g m−2 year−1, turnover rate 1.06 year−1; 2004 Conversion stand: production 164.2 g m−2 year−1, turnover rate 0.79 year−1).

Involvement of lignin and hormones in the response of woody poplar taproots to mechanical stress

Mechanical stress is a widespread condition caused by numerous environmental factors that severely affect plant stability. In response to mechanical stress, plants have evolved complex response pathways able to detect mechanical perturbations and inducing a suite of modifications in order to improve anchorage. The response of woody roots to mechanical stresses has been studied mainly at the morphological and biomechanical level, whereas investigations on the factors triggering these important alterations are still at the initial stage. Populus has been widely used to study the response of stem to different mechanical stresses and, since it has the first forest tree genome to be decoded, represents a model woody plant for addressing questions on the mechanisms controlling adaptation of woody roots to changing environments. In this study, a morphological and physiological analysis was used to investigate factors controlling modifications in Populus nigra woody taproots subjected to mechanical stress. An experimental model analyzing spatial and temporal mechanical force distribution along the woody taproot axis enabled us to compare the events occurring in its above-, central- and below-bending sectors. Different morphogenetic responses and local variations of lignin and plant hormones content have been observed, and a relation with the distribution of the mechanical forces along the stressed woody taproots is hypothesized. We investigated the differences of the response to mechanical stress induction during the time; in this regard, we present data referring to the effect of mechanical stress on plant transition from its condition of winter dormancy to that of full vegetative activity.

Fine-root carbon and nitrogen concentration of European beech (Fagus sylvatica L.) in Italy Prealps: possible implications of coppice conversion to high forest

Fine-root systems represent a very sensitive plant compartment to environmental changes. Gaining further knowledge about their dynamics would improve soil carbon input understanding. This paper investigates C and N concentrations in fine roots in relation to different stand characteristics resulting from conversion of coppiced forests to high forests. In order to evaluate possible interferences due to different vegetative stages of vegetation, fine-root sampling was repeated six times in each stand during the same 2008 growing season. Fine-root sampling was conducted within three different soil depths (0–10; 10–20; and 20–30 cm). Fine-root traits were measured by means of WinRHIZO software which enable us to separate them into three different diameter classes (0–0.5, 0.5–1.0 and 1.0–2.0 mm). The data collected indicate that N concentration was higher in converted stands than in the coppiced stand whereas C concentration was higher in the coppiced stand than in converted stands. Consequently the fine-root C:N ratio was significantly higher in coppiced than in converted stands and showed an inverse relationship with fine-root turnover rate, confirming a significant change of fine-root status after the conversion of a coppice to high forest.

MicroRNAs expression patterns in the response of poplar woody root to bending stress

AbstractMain conclusionThe paper reports for the first time, in poplar woody root, the expression of five mechanically-responsive miRNAs. The observed highly complex expression pattern of these miRNAs in the bent root suggest that their expression is not only regulated by tension and compression forces highlighting their role in several important processes, i.e., lateral root formation, lignin deposition, and response to bending stress. Mechanical stress is one of the major abiotic stresses significantly affecting plant stability, growth, survival, and reproduction. Plants have developed complex machineries to detect mechanical perturbations and to improve their anchorage. MicroRNAs (miRNAs), small non-coding RNAs (18–24 nucleotides long), have been shown to regulate various stress-responsive genes, proteins and transcription factors, and play a crucial role in counteracting adverse conditions. Several mechanical stress-responsive miRNAs have been identified in the stem of Populus trichocarpa plants subjected to bending stress. However, despite the pivotal role of woody roots in plant anchorage, molecular mechanisms regulating poplar woody root responses to mechanical stress have still been little investigated. In the present paper, we investigate the spatial and temporal expression pattern of five mechanically-responsive miRNAs in three regions of bent poplar woody taproot and unstressed controls by quantitative RT-PCR analysis. Alignment of the cloned and sequenced amplified fragments confirmed that their nucleotide sequences are homologous to the mechanically-responsive miRNAs identified in bent poplar stem. Computational analysis identified putative target genes for each miRNA in the poplar genome. Additional miRNA target sites were found in several mechanical stress-related factors previously identified in poplar root and a subset of these was further analyzed for expression at the mRNA or protein level. Integrating the results of miRNAs expression patterns and target gene functions with our previous morphological and proteomic data, we concluded that the five miRNAs play crucial regulatory roles in reaction woody formation and lateral root development in mechanically-stressed poplar taproot.

The influence of slope on Spartium junceum root system: morphological, anatomical and biomechanical adaptation

Root systems have a pivotal role in plant anchorage and their mechanical interactions with the soil may contribute to soil reinforcement and stabilization of slide-prone slopes. In order to understand the responses of root system to mechanical stress induced by slope, samples of Spartium junceum L., growing in slope and in plane natural conditions, were compared in their morphology, biomechanical properties and anatomical features. Soils sampled in slope and plane revealed similar characteristics, with the exception of organic matter content and penetrometer resistance, both higher in slope. Slope significantly influenced root morphology and in particular the distribution of lateral roots along the soil depth. Indeed, first-order lateral roots of plants growing on slope condition showed an asymmetric distribution between up- and down-slope. Contrarily, this asymmetric distribution was not observed in plants growing in plane. The tensile strength was higher in lateral roots growing up-slope and in plane conditions than in those growing down-slope. Anatomical investigations revealed that, while roots grown up-slope had higher area covered by xylem fibers, the ratio of xylem and phloem fibers to root diameter did not differ among the three conditions, as also, no differences were found for xylem fiber cell wall thickness. Roots growing up-slope were the main contributors to anchorage properties, which included higher strength and higher number of fibers in the xylematic tissues. Results suggested that a combination of root-specific morphological, anatomical and biomechanical traits, determines anchorage functions in slope conditions.

Drought and fire stress influence seedling competition in oak forests: fine-root dynamics as indicator of adaptation strategies to climate change

Increased summer drought and wildfires as a consequence of continuing climate change are expected to lead to disturbance of Mediterranean ecosystems. Seedlings recruitment is sensitive to both stresses and, therefore, any adaptation and restoration strategy devised to protect these forests should take into account a careful study on their effects on seedling development. As a substantial fraction of net primary productivity of forested ecosystems is channelled in the belowground compartments, the knowledge of how roots behave under stressful conditions becomes of primary importance to select the right management strategy to be implemented. This work tries to enlighten the events occurring in the fine root portion of the root system in young seedlings of three co-existing oak species (Quercus ilex, Quercus trojana and Quercus virgiliana) under controlled conditions. We have made a comparative analysis of the effect of these two stresses, alone or in combination, with the aim to evaluate the tolerance level of these seedlings and, therefore, to obtain an indication of their recruitment potential in the field. The parameters investigated were biomass and a number of morphological traits. Data obtained suggest that a decrease in diameter could be part of a tolerance strategy in all three oaks tested together with a reduction of root length. In addition, tolerance to water shortage could require a reduction of carbon allocated belowground, in particular in the very fine roots, which leads to an overall reduction of the root system dimension. Q. trojana seedlings seem to be the fastest in resuming growth after stress interruption but a good recovery was also found for the remaining two oak species. Although our study provides interesting information regarding a possible tolerance strategy taking place in the fine root compartment when seedlings of these three oak species undergo water stress and fire treatment, more information is needed before any suggestion can be made as to which species would be best suited to make these forests more resistant to global changes.

Interspecific variation in functional traits of oak seedlings (Quercus ilex, Quercus trojana, Quercus virgiliana) grown under artificial drought and fire conditions.

To face summer drought and wildfire in Mediterranean-type ecosystems, plants adopt different strategies that involve considerable rearrangements of biomass allocation and physiological activity. This paper analyses morphological and physiological traits in seedlings of three oak species (Quercus ilex, Quercus trojana and Quercus virgiliana) co-occurring under natural conditions. The aim of this study was to evaluate species-specific characteristics and the response of these oak seedlings to drought stress and fire treatment. Seedlings were kept in a growth chamber that mimicked natural environmental conditions. All three species showed a good degree of tolerance to drought and fire treatments. Differences in specific biomass allocation patterns and physiological traits resulted in phenotypic differences between species. In Q. ilex, drought tolerance depended upon adjustment of the allocation pattern. Q. trojana seedlings undergoing mild to severe drought presented a higher photosystem II (PSII) efficiency than control seedlings. Moreover, Q. trojana showed a very large root system, which corresponded to higher soil area exploitation, and bigger leaf midrib vascular bundles than the other two species. Morphological and physiological performances indicated Q. trojana as the most tolerant to drought and fire. These characteristics contribute to a high recruitment potential of Q. trojana seedlings, which might be the reason for the dominance of this species under natural conditions. Drought increase as a result of climate change is expected to favour Q. trojana, leading to an increase in its spatial distribution.

New branch roots produced by vascular cambium derivatives in woody parental roots of Populus nigra L.

Abstract In literature, it has been suggested that new branch roots are produced in a woody parental along its axis not only in the proximal zone where a primary anatomical structure is present but even where old branch roots are present, and a thick production of wood internally characterizes the parental root anatomy. This paper confirms this hypothesis by showing, for the first time, the presence of a new branch root primordium forming within the secondary phloem tissues of a woody parental root. The new branch root primordia found in this work are designated secondary branch roots (SBRs) and seem to develop from derivatives of the vascular cambium that abandon the formation of conducting elements to become the mother cells of these new root primordia. We find that traces belonging to SBRs present some anatomical difference compared with those belonging to branch roots deriving from primary tissues designated, according to literature, primary branch roots (PBRs). This difference in traces could help to distinguish between the origin of the SBRs and PBRs present along a woody parental axis, and this could be helpful in understanding how the root system of a woody plant is developed. The possibility of branching, wherever needed, along the root axes, independently of their internal anatomical organization, evokes a new scenario in which woody plants, in analogy with herbaceous plants, continuously modify their root system in an attempt to adapt and to better exploit their rooting environment.

Poplar woody root proteome during the transition dormancy-active growth

Woody plants living in temperate climates finely regulate their growth and development in relation to seasonal changes; their transition from vegetative to dormancy phase represents an adaptation to their environment. Events occurring in the shoot during onset/release from dormancy have been largely investigated, whereas in woody roots they remain completely unknown. In recent years, we have been interested in understanding the molecular and physiological events occurring in poplar woody root during release from dormancy. Here, we propose the results of a comparative analysis of the proteome of poplar woody root sampled at different time points: T0 (dormancy condition), T1 (release from dormancy), and T2 (full vegetative condition). This study identified proteins that may be involved in the long-term survival of a dormant root or landmarking a specific time point.

Non-destructive Phenotypic Analysis of Early Stage Tree Seedling Growth Using an Automated Stereovision Imaging Method

A plant phenotyping approach was applied to evaluate growth rate of containerized tree seedlings during the precultivation phase following seed germination. A simple and affordable stereo optical system was used to collect stereoscopic red–green–blue (RGB) images of seedlings at regular intervals of time. Comparative analysis of these images by means of a newly developed software enabled us to calculate (a) the increments of seedlings height and (b) the percentage greenness of seedling leaves. Comparison of these parameters with destructive biomass measurements showed that the height traits can be used to estimate seedling growth for needle-leaved plant species whereas the greenness trait can be used for broad-leaved plant species. Despite the need to adjust for plant type, growth stage and light conditions this new, cheap, rapid, and sustainable phenotyping approach can be used to study large-scale phenome variations due to genome variability and interaction with environmental factors.