Antonio Valera Espín
University of Murcia
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Featured researches published by Antonio Valera Espín.
Journal of Plant Physiology | 1994
M. E. Candela; R. Muñoz; María D. Alcázar; Antonio Valera Espín
Summary Cucumber mosaic virus (CMV) infection of Capsicum annuum leaves provokes a strong increase in soluble peroxidase activity. Isoelectrofocusing of the peroxidase activity in polyacrylamide gel patterns, after staining the soluble protein fractions from both healthy and infected leaves with benzidine/H 2 O 2 , revealed the presence of two acidic and one basic isoperoxidases, the increase in peroxidase activity being mainly due to the former. Staining of peroxidase activity in the gels with coniferyl alcohol/H 2 O 2 showed that acidic isoenzymes were more capable of catalyzing coniferyl alcohol oxidation than basic isoenzymes. Native polyacrylamide gel electrophoresis of the soluble fraction showed that the increase in peroxidase isoenzymes. CMV elicitation of the acidic isoperoxidase through its involvement in the lignification process might be considered as a part of the defense mechanism of Capsicum annuum against viral infection, lignin deposition on plasmodesmata could act as a physical barrier against the cell-to-cell advance of viral particles.
Biologia Plantarum | 1993
M. E. Candela; Antonio Valera Espín; María D. Alcázar
Eight major pathogenesis related (PR) proteins were found in a soluble extract of cucumber mosaic virus (CMV) infectedCapsicum annuum leaves. None of them was present in the soluble extract of sham-inoculated controls. The proteins were detected by two-dimensional polyacrylamide gel electrophoresis using (2D-PAGE)PhastSystem, native-PAGE in the first dimension and SDS-PAGE in the second. Two major acidic PR-proteins were identified on the basis of their relative molecular mass (Mr), PR1 of 15.3 kD and PR2 of 29 kD. Six proteins were basic and were identified as PRla of 15.9 kD, PRlb of 15.0 kD, PRlc of 15.9 kD, PR2 of 27.0 kD, PR3 of 36.3 kD and PR4 of 48.8 kD.
Physiologia Plantarum | 1995
M. Dolores Alcázar; Catalina Egea; Antonio Valera Espín; M. Emilia Candela
Anales De Psicologia | 1997
Antonio Valera Espín; Julio Sánchez Meca
Psicothema | 2000
Antonio Valera Espín; Julio Sánchez Meca; Fulgencio Marín Martínez
Anales De Psicologia | 1992
Julio Sánchez Meca; Antonio Valera Espín; Antoino P. Velandrino Nicolás; Fulgencio Marín Martínez
Revista de psicología general y aplicada: Revista de la Federación Española de Asociaciones de Psicología | 1998
Antonio Valera Espín; Julio Sánchez Meca; Fulgencio Marín Martínez; Antonio Pablo Velandrino Nicolás
Anales De Psicologia | 1992
Manuel Ato García; José Miguel Nieto Cano; Antonio Valera Espín; Antonio Portela Pruaño
Anales De Psicologia | 1992
Fulgencio Marín Martínez; Antoino P. Velandrino Nicolás; Antonio Valera Espín; Julio Sánchez Meca
Archive | 2000
Antonio Valera Espín; Julio Sánchez Meca; Fulgencio Marín Martínez