Arkadiusz Terman

Associations between the prolactin receptor gene polymorphism and reproductive traits of boars

The prolactin receptor gene (PRLR), located on chromosome 16 in pigs, is a candidate gene for reproductive traits. The experiment was aimed to detect the DNA mutations in this gene and to find probable relations between the genotype and some reproductive traits in boars. The polymorphism in thePRLR gene was identified by PCR-RFLP method using specific primers and the restriction enzymeAluI. In total 229 boars of various breeds were genotyped. The frequency of allele A was estimated at 0.62 and allele B at 0.38. Genotype AA was found at a frequency of 0.45, AB at 0.35 and BB at 0.20. We found associations betweenPRLR genotype and ejaculate volume, sperm concentration, percentage of live sperm, and number of live sperm in the ejaculate (P < 0.01).

The IGF1R gene: A new marker for reproductive performance traits in sows?

Abstract The goal of this study was to determine if there is any association between the DNA mutations in the insulin-like growth factor-1 receptor gene and litter size in Polish sows. The following reproductive traits were investigated: total number of piglets born (TNB), number of piglets born alive (NBA), and number of piglets weaned. The polymorphism in the IGF1R gene was analyzed by a PCR-RFLP method using the restriction enzymes SacII. Two different alleles of the IGF1R gene were identified. The statistical analysis in first parity showed that the BB genotype was superior and the differences were highly significant (p≤0.01). Later parities sows with the BB genotype still had the largest litter size compared to AB and AA sows, but the difference was statistically not significant. The preliminary study suggests a possibility of using the IGF1R gene polymorphisms to improvement of litter size of sows.

Association between prolactin receptor (PRLR) gene polymorphism and reproduction performance traits of Polish swine

Abstract The aim of this study was to estimate the relationship between prolactin receptor (PRLR) gene polymorphism and litter size in pigs of synthetic line 990 (n = 374). Prolactin receptor gene variants were determined by PCR–RFLP method. It was demonstrated that the analyzed polymorphism had significant influence (P ≤ 0.05) on reproduction traits in the first parity.

Association between GPX5 gene polymorphism and selenium concentration in liver and kidney of wild boars from West Pomerania province, Poland

The aim of the experiment was to detect polymorphism in the GPX5 gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in GPX5 gene was detected using the PCR-RFLP method with specific primers and the restriction enzyme Hinf I. Two different alleles of the GPX5 gene were identified - allele 1B (0.37) and 2B (0.63). The relationships between the GPX5 genotypes and concentrations of selenium in liver and kidney were analyzed, revealing statistically significant (P≤0.05) differences between wild boars carrying different GPX5 genotypes in both genders. Obtained results indicated that wild boars with the 2B2B genotype had the highest selenium concentration in livers and kidneys compared to 1B1B and 1B2B animals and these differences were statistically significant (P≤0.05).