Asaichiro Ozaki

Microbial Production of Salicylic Acid from Naphthalene: Part I. Isolation of the High Yield Strain and the Flask Scale Culture

The high yield strain was isolated by the direct selection process using the naphthalene plate prepared by a new technique. The highest conversion rate of 94 weight per cent was obtained by studying the relationship among factors which affect the salicylate accumulation and degradation pattern. The almost complete conversion seems to be a result of inactivity of the salicylate oxidation enzyme until the exhaustion of naphthalene. The authors found it possible for the high-concentrated accumulation to neutralize the acidity of the accumulated salicylic acid indirectly by the addition of urea, instead of alkali or high-concentrated phosphate.

Bacteriophages of l-Glutamic Acid-Producing Bacteria: Part XI. Selection of Inhibitors for Phage Infection and Suppression of Phage Adsorption by Phytic AcidPart XII. Inhibition of Brevibacterium Phages by Non-Ionic Surface-Active Agents

Antiphage properties of many kinds of chemicals such as antibiotics, surface-active agents and chelating agents were examined on Brevibacterium lactofermentum No. 2256—phage P465 system using double-layer agar method, as a part of the basic study, for preventing phage infection in the industrial fermentation.A great majority of inhibitors which were selected were usually nonspecific and inhibited also bacterial growth. Among about 200 chemicals tested, 5 antibiotics such as chloramphenicol and tetracycline, 6 chelating agents such as phytic acid and 19 surface- active agents such as PEG monoester and POE alkyl ether showed the selective inhibitions for phage infection at the concentrations which did not affect bacterial growth, or at the subbactericidal concentrations that suppressed bacterial growth slightly.Of the above chemicals which showed selective inhibitions for phage infection, a possible mechanism of chelating agents chiefly of phytic acid was investigated. When 0.1 to 0.2% of phytic acid was pr...