Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Ashok Aggarwal is active.

Publication


Featured researches published by Ashok Aggarwal.


Journal of Soil Science and Plant Nutrition | 2012

Impact of different levels of superphosphate using arbuscular mycorrhizal fungi and Pseudomonasfluorescens on Chrysanthemum indicum L

K Prasad; Ashok Aggarwal; Kuldeep Yadav; Anju Tanwar

A pot experiment was conducted to investigate the potential effect of arbuscular mycorrhizal fungi (Glomus mosseae & Acaulospora laevis) and phosphate solubi- lizing bacteria (Pseudomonas fluorescens) with different levels of superphosphate on Chrysanthemum indicum L. After 100 days, different plant growth parameters such as mycorrhizations characteristics, phosphatase activity and phosphorus up- take were measured. The obtained results revealed that the inoculation of plants with biofertilizers and recommended dose of superphosphate significantly improved the growth parameters. Inoculation with A. laevis + P. fluorescens at medium con- centration of superphosphate showed maximum height, fresh and dry root weight, AM root colonization, AM spore count, alkaline phosphatase activity, acidic phos- phatase activity and the percent phosphorus uptake in shoot and root whereas root length was maximum in G. mosseae + A. laevis + P. fluorescens. Leaf area and fresh and dry shoot weight were maximum in the treatment (G. mosseae + A. laevis + P. fluorescens) at low concentration of superphosphate. The use of AMF increased nu- trient acquisition from an organic fertilizer source by enhancing acidic phosphatase (ACP) and alkaline phosphatase (ALP) activity, thus facilitating P acquisition and improving plant growth.


Journal of Soil Science and Plant Nutrition | 2013

Arbuscular mycorrhizal inoculation and super phosphate application influence plant growth and yield of Capsicum annuum

Anju Tanwar; Ashok Aggarwal; Nisha Kadian; A. Gupta

The effect of two arbuscular mycorrhizal fungi [G. mosseae (G) and A. laevis (A)] with P. fluorescence (Pf) in the presence of super phosphate (P) fertilization on growth and yield of bell pepper (Capsicum annuum var. California Wonder) was evaluated in pots under greenhouse conditions, in a completely randomized design with four levels of phosphorus fertilizer [F0–without P, F1–0.200g pot -1 (half of the recommended dose), F3–0.400g pot -1 (recommended dose) and F4–0.800g pot -1 (double the recommended dose)] having six different combinations of bioinoculants. Inoculation of bioinoclulants with F1 increased plant growth and nutrition to an acceptable level with AM fungi in combination with P. fluorescens. Application of higher dose of P fertilizer markedly decreased all the growth parameters. The prevalence of AM colonization was highest in G+A+Pf with F1. Similarly highest yield was recorded for the treatment involving multi inoculation of G+A+Pf in the treatment of F1 followed by dual inoculation of G+Pf in F0 plants. Thus this finding suggests the application of efficient bioinoculants (G+A+Pf) along with right dose of P fertilizer (half of the recommended P) during seedling transplantation to increase overall growth and yield performance of bell pepper and could be considered as a sustainable substitute to higher phosphorus fertilizer for bell pepper cultivation.


Fitoterapia | 2013

Evaluation of genetic fidelity among micropropagated plants of Gloriosa superba L. using DNA-based markers — a potential medicinal plant

Kuldeep Yadav; Ashok Aggarwal; Narender Singh

Malabar glory lily (Gloriosa superba L.) is a medicinally potent plant species used for the production of alkaloid colchicine. With ever increasing demand, there is a pressing need to conserve it through biotechnological approaches. A large number of complete plantlets were obtained by direct regeneration from the non-dormant tuber explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg/l 6-benzylaminopurine (BAP)+0.5 mg/l α-naphthalene acetic acid (NAA). Large number of plants can be produced in vitro under aseptic conditions, but there is always a danger of producing somaclonal variants by tissue culture technology. Thus, the genetic stability of micropropagated clones was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis. During the study a total of 80 (50 RAPD and 30 ISSR) primers were screened, out of which 10 RAPD and 7 ISSR primers produced a total of 98 (49 RAPD and 49 ISSR) clear, distinct and reproducible amplicons. The amplification products of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. This is the first report that evaluates the use of genetic markers to establish genetic fidelity of micropropagated G. superba using RAPD and ISSR, which can be successfully applied for the mass multiplication, germplasm conservation and further genetic transformation assays for colchicine production to meet the ever increasing demand of this medicinally potent plant for industrial and pharmaceutical uses.


Journal of Crop Science and Biotechnology | 2012

Actions for ex situ conservation of Gloriosa superba L. - an endangered ornamental cum medicinal plant

Kuldeep Yadav; Ashok Aggarwal; Narender Singh

Factors affecting in vitro propagation and microtuberization were evaluated for Gloriosa superba L., an endangered ornamental cum medicinal plant having limited reproductive capacity. Surface sterilization of tuber explants with 0.1% mercuric chloride (HgCl2) for 5 min eliminated the contamination effectively with highest survival rate. Among the various combinations used, Murashige and Skoog (MS) medium with 2.0 mg L−1 6-benzylaminopurine (BAP) + 0.5 mg L−1 α-naphthalene acetic acid (NAA) containing 3% sucrose with 16-h photoperiod exhibited the greatest in vitro tuberization (3.2) with the highest shoot regeneration frequency (90%). The longest tuber regeneration occurred on MS media containing 4% sucrose. Transfer of in vitro-regenerated shoots to half-strength MS medium with 1.0 mg L−1 indole-3-butyric acid (IBA) + 0.5 mg L−1 NAA showed maximum root induction (66.6%). The in vitro-grown plantlets were successfully acclimatized and transplanted to sterilized soil and sand mixture (3:1) in the glasshouse with 70% survival. The colchicine content was determined in the tubers of ex vitro plants by HPLC using the same retention time (1.5 min) as that of the standard colchicine. This revealed that the micropropagation protocol developed by us for rapid mass production could be used as raw material for colchicine extraction and provides a basis for germplasm conservation and genetic improvement of G. superba.


Biological Agriculture & Horticulture | 2013

Screening and selection of efficient host and sugarcane bagasse as substrate for mass multiplication of Funneliformis mosseae

Anju Tanwar; Ashok Aggarwal; Alpa Yadav; Vipin Parkash

This investigation reports a novel and low-cost method of inoculum production of arbuscular mycorrhizal (AM) fungus, Funneliformis mosseae (sensu Glomus mosseae), using an organic medium. The experiment is a 3 × 3 × 4 factorial design employing three hosts, maize (Zea mays L.), lemon grass (Cymbopogon nardus (L.) Rendle) and palmarosa (Cymbopogan martini (Roxb.) Wats.), three forms of sugarcane bagasse substrate (fresh, dry and compost) and four different concentrations of each substrate (without substrate, 25, 50 and 100 g pot− 1). Mass multiplication of F. mosseae was measured in terms of AM spore number and per cent root colonization. The plant growth was monitored in terms of plant height, above-ground fresh and dry weights and root fresh and dry weights. All three grasses tested varied in their tendency to be colonized by F. mosseae and in spore formation. Maize was found to be the most appropriate host followed by lemon grass and palmarosa. In the case of maize, a positive relationship was observed between spore number and root colonization rate and compost bagasse with highest concentration, 100 g, resulted in the greatest multiplication of F. mosseae. Maize with 100 g compost bagasse, lemon grass with 25 g of dry and 50 g of compost bagasse and palmarosa with 100 g compost and fresh bagasse showed greatest increase in plant growth.


Russian Agricultural Sciences | 2013

AM fungi ameliorates growth, yield and nutrient uptake in Cicer arietinum L. Under salt stress

Nisha Kadian; Kuldeep Yadav; Neetu Badda; Ashok Aggarwal

A pot experiment was performed to see the effect of two indigenous arbuscular mycorrhizal (AM) fungi Glomus mosseae and Acaulospora laevis, alone and in combination on growth, biomass and mineral nutrition of Cicer arietinum under different salinity levels imposed by 4, 8, and 12 dS/m EC solution (sodium chloride, calcium chloride and sodium sulphate). All AM inoculated plants showed significant increment in growth, biomass, mineral nutrition and yield over control. The extent of bioinoculants response on plant growth as well as root colonization decreases with the increase in the level of salinity. Among all the growth parameters plant height (26.4 ± 1.14 cm), root length (13.4 ± 1.67 cm), total chlorophyll (2.33 ± 0.02 mg/100 mg f. wt), root colonization (41.6 ± 2.70%) and AM spore number (63.8 ± 1.78 per 10 gm soil) were recorded highest in dual combination (G. mosseae and A. laevis) at 4 dS/m while fresh shoot (6.80 ± 0.67 gm) and dry shoot weight (0.96 ± 0.18 gm) was found maximum in G. mosseae at the same concentration of salinity. Greater Phosphorus (P) acquisition and yield was observed at 4 dS/m with dual combination (G. mosseae and A. laevis) that possibly be responsible to protect plants from salt stress. Although Nitrogen (N), Potassium (K) and Sodium (Na) contents also declined with increasing salinity. Overall results showed that mycorrhizal colonization improves host plant mineral concentration and thereby increases the growth, yield and nutrient uptake of C. arietinum ameliorating the harmful effect at salinity stress.


International Journal of Vegetable Science | 2013

Biological Amendments on Growth, Nutritional Quality, and Yield of Celery

Anju Tanwar; Kuldeep Yadav; Karishma Prasad; Ashok Aggarwal

Soil nutrients can decrease during organic production of celery (Apium graveolens L.) transplants. Arbuscular mycorrhizal fungi (AMF) are known to promote plant nutrition and growth. Hence, a study was carried out to determine the potential of AMF [Funneliformis mosseae (Nicol. and Gerd.) Walker and Schüβler and Acaulospora laevis Gerd. and Trappe], used alone or in combination with the bacterium Pseudomonas fluorescens and/or the nonmycorrhizal fungus Trichoderma viride Pers. on celery in a greenhouse. The objective was to select an efficient AMF, or other growth-promoting microbes, for soil inoculation to improve establishment and growth of celery transplants. Inoculation with a combination of F. mosseae + P. fluorescens produced increased root length and fresh and dry weights, and the best plant height was obtained when all of the bioinoculants were used together. Mycorrhizal root colonization increased when P. fluorescens was used and the combination of F. mosseae + P. fluorescens produced the most colonization. Inoculation with F. mosseae + P. fluorescens increased shoot and root P content and root N content and increased celery yield by increasing leafstalk length. Higher photosynthetic rate, chlorophyll content, and shoot N content occurred when F. mosseae + A. laevis + P. fluorescens was used, with improved numbers of leafstalks, leaf area, and basal disc diameter. Soil amended with F. mosseae + P. fluorescens and F. mosseae + A. laevis + P. fluorescens at transplanting stage increased production of high-quality celery stalks with better nutrition and reduced fertilizer application.


New Zealand Journal of Crop and Horticultural Science | 2014

Effect of bioinoculants and superphosphate fertilizer on the growth and yield of broccoli (Brassica oleracea L. var. italica Plenck)

Anju Tanwar; Ashok Aggarwal; Vipin Parkash

A glasshouse pot experiment was conducted to investigate the effect of two species of arbuscular mycorrhizal fungi (Glomus intraradices and Acaulospora laevis) and Pseudomonas fluorescens under three levels of superphosphate fertilizer (half dose, 40 kg/ha; recommended dose, 80 kg/ha; and double dose, 160 g/ha) on the growth and yield of broccoli (Brassica oleracea var. italica). There was negligible colonization of broccoli roots by the mycorrhizal fungi, demonstrating that they have no role in the growth of this plant. However, the strain of P. fluorescens used (MTCC No. 103) was found to have a potential role in enhancing the growth, phosphatase activity, chlorophyll content, nutrient uptake and yield of broccoli when combined with the recommended dose of fertilizer. By contrast, P. fluorescens combined with a half or double dose of fertilizer led to poor growth and delayed head formation. This suggests that root inoculation with P. fluorescens (MTCC No. 103) will improve plant growth, nutrient uptake and yield of broccoli when combined with the recommended dose of superphosphate fertilizer.


Journal of Plant Interactions | 2014

Multifaceted potential of bioinoculants on red bell pepper (F1 hybrid, Indam Mamatha) production

Anju Tanwar; Ashok Aggarwal

The present investigation was undertaken to determine the comparative efficacy of two arbuscular mycorrhizal (AM) fungi (Funneliformis mosseae and Acaulospora laevis) with Trichoderma viride and Pseudomonas fluorescens on growth and yield of red bell pepper. The results indicate that F. mosseae colonized the plant roots better as compared to A. laevis and promoted maximum increment in AM spore number, root colonization, leaf area, acid phosphatase activity, early fruit formation along with maximum increase in fruit nitrogen, and protein content. Whereas F. mosseae+P. fluorescens promoted maximum increase in plant height, shoot weight, mycorrhizal dependency, chlorophyll a, alkaline phosphatase activity, and fruit phosphorus content. Regarding root length, root weight, leaf photosynthesis, chlorophyll b, number of fruits per plant and their fresh weight, it was found best in F. mosseae+A. laevis+P. fluorescens. Therefore, soil inoculation with suitable bioinoculant should be used at nursery stage for better yield.


Chilean Journal of Agricultural Research | 2012

Influence of Arbuscular Mycorrhizal Fungi and Pseudomonas fluorescens at Different Superphosphate Levels on Linseed (Linum usitatissimum L.) Growth Response

Neetu Neetu; Ashok Aggarwal; Anju Tanwar; Alpa Alpa

The aim of this study was to investigate the influence of arbuscular mycorrhizal fungi (AMF) Glomus mosseae (T.H. Nicolson & Gerd.) Gerd. & Trappe and Acaulospora laevis (Gerd. & Trappe) on linseed (Linum usitatissimum L.) growth response with phosphate solubilizing bacteria Pseudomonas fluorescens; different doses of superphosphate were used: 20 kg ha -1 (half recommended dose), 40 kg ha -1 (recommended dose), and 80 kg ha -1 (double recommended dose) in earthen pots filled with sterilized soil under greenhouse conditions. Among all the growth parameters, the following were the highest in the G. mosseae + P. fluorescens combination at the medium concentration (recommended superphosphate dose): plant height (78.74 ± 1.8 cm), fresh shoot weight (3.45 ± 0.294 g), dry shoot weight (0.57 ± 0.007 g), fresh root weight (0.223 ± 0.023 g), dry root weight (0.036 ± 0.004 g), root length (17.67 ± 0.48 cm), AM spore number (94.4 ± 9.86), shoot (1.14 ± 0.115%) and root (1.29 ± 0.110%) P content, and acidic (0.447 ± 0.012 IU g -1 FW) and alkaline phosphatase activity (0.119 ± 0.008 IU g -1 FW). The percentage mycorrhizal root colonization with the A. laevis + P. fluorescens (86.86 ± 2.17%) combination and chlorophyll content with the G. mosseae + A. laevis + P. fluorescens (0.474 ± 0.009 mg g -1 FW) combination recorded the highest values at the low concentration (half recommended superphosphate dose) as compared with non-mycorrhizal plants (control). The high superphosphate dose clearly reduced or decreased all the growth parameters. Therefore, vigorous growth and maximum flax yield can be achieved by inoculating plants with AM fungi and P. fluorescens with the recommended dose or less than the recommended dose of superphosphate.

Collaboration


Dive into the Ashok Aggarwal's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar

Anju Tanwar

Kurukshetra University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Vipin Parkash

Rain Forest Research Institute

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Neetu Badda

Kurukshetra University

View shared research outputs
Researchain Logo
Decentralizing Knowledge