Asiye İzem Sandal

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

ABSTRACT This study aimed to investigate the effects of different doses of taurine added to glucose extender. Semen was collected from rainbow trout (Oncorhynchus mykiss) by abdominal massage and diluted (1:2) with 300 mM glucose (G) extender containing different taurine doses (50, 75 and 100 mM). The control group extender did not contain taurine. After dilution and dosing, samples were filled to 0.5 ml straws, frozen in nitrogen vapour and stored in liquid nitrogen. The comparison of the results obtained for the 50 mM taurine group and the control group showed no significant difference between the post-thawing motility percentages and motility duration of sperm, and only a very low statistically difference for eyed-embryo percentages (P < 0.05).

Kedilerde MI ve MII Oositleri Kullanilarak Somatik Klonlama

Animal production via SCNT provides a unique tool for protection of valuable individuals, conservation of vulnerable and endangered species and production of transgenic animals. A total of 167 MI and 219 MII stage oocytes were used as the material of the study. The oocytes were enucleated at 44 h after in vitro maturation by aspiration of the polar body and the MI or MII plates. Cycling granulosa cells were used for nuclear transfer. Cell fusion was induced with DC pulses of 2.0 kV/cm 60µs, 0.1s apart (2x) delivered by a BTX Electrocell Manipulator 200 (BTX, San Diego, CA, USA). After fusion, the embryos were activated by 1.0 kV/cm 20µs DC pulses 0.1s apart (2x) followed by 2 mM 6-DMAP (6-dimethylaminopurine) incubation in culture medium for 4 h in a humidified atmosphere of 5% CO2, 5% O2, and 90% N2 at 38°C. The somatic cell transferred embryos were cultured for 8 days in mSOF medium supplemented with 0.4% BSA in a humidified 5% CO2, 5% O2, and 90% N2 atmosphere at 38°C. After in vitro culture period, all embryos transferred to HSOF containing Hoechst 33342 (5 μg/mL) and the cell numbers were counted under ultraviolet light using a fluorescent microscope. The fusion (66.66 vs 21.55%) and cleavage rates (15.75 vs 11.11%) were significantly higher in MII stage oocytes than MI stage oocytes (P<0.02). While SCNT embryos were developed to morula stage in MII group (14; 9.58%), all the cleaved embryos were arrested at the 2-4 cell stage in MI group. None of the embryos was developed to blastocyst stage in both groups.

The effect of oviductal cells on in vitro maturation of canine oocytes in different culture media

Sinem Özlem ENGİNLER*, Asiye İzem SANDAL, Özen Banu ÖZDAŞ, Ramazan ARICI, Ezgi ERTÜRK, Elif Merve ÇINAR, Israa Faris MOHAMMED, Alper BARAN, Çağatay TEK, Mehmet Can GÜNDÜZ Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Istanbul University, Avcılar, İstanbul, Turkey Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, Avcılar, İstanbul, Turkey