Sinem Özlem Enginler

Genetic variations of BRCA1 and BRCA2 genes in dogs with mammary tumours

Mammary tumours are the most common tumour type in female dogs. The formation of the mammary tumours is multifactorial but the high incidence of tumour disease in certain canine breeds suggests a strong genetic component. BRCA1 and BRCA2 are the most important genes significantly associated with mammary tumours. The aim of this study was to determine the association between the variations of these two genes and canine mammary tumours. 5′-untranslated region, intron 8 and exon 9 of BRCA1 and exons 12, 24, 27 of BRCA2 were sequenced in order to detect the genetic variations. In addition to six previously identified polymorphisms, six novel single nucleotide polymorphisms (SNPs) were detected. Five of the coding SNPs were synonymous and three of them were non-synonymous. The comparison of the sequences from 25 mammary tumour bearing and 10 tumour free dogs suggested that the two SNPs in intron 8 and exon 9 of BRCA1 and two SNPs in exon 24 and exon 27 of BRCA2, which are firstly identified in this study, might be associated with mammary tumour development in dogs. Especially one SNP in exon 9 of BRCA1 and one SNP in exon 24 of BRCA2 were found to be significantly associated with canine mammary tumours.

Bir Köpekte Ovarian Granüloza Hücre Tümörü ve Kompleks Meme Adenokarsinomu

On bir yasli boxer kopek rutin kisirlastirma icin Dogum ve Jinekoloji Anabilim Dali klinigine getirildi ve histopatolojik olarak granuloza hucre tumoru (GHT) olarak teshis edilen bir kitle tesadufen saptandi. Ameliyat esnasinda hormonal analiz icin kan ornegi toplandi ve bu kan orneginde ostrojen konsantrasyonu referans degerlerin altinda, progesteron konsantrasyonu ise normal olarak tespit edildi. Kopegin ilk muayenesi esnasinda meme bezlerinde genislemeler tespit edildi ve herhangi bir vulvar akinti/sislik tespit edilmedi. Kisirlastirmayi takiben bir ay sonra kopek total bilateral mastektomi icin klinigimize tekrar getirildi. Histopatolojik olarak tumorel kitleler kompleks adenokarsinom tip II olarak tespit edildi. Kopekte hem GHT hem de meme tumoru ayni anda bulundugu halde, GHT’nun kopek meme tumoru gelisimi uzerine etkisi tam anlamiyla anlasilamadi. Serum ostrojen ve progesteron konsantrasyonlarinin referans degerlerden yuksek olmamasina bagli olarak, GHT’nun henuz hormon salgilamadigi dusunuldu. Sonuc olarak, bu olgu GHT’nun kopek meme tumorunun gelisimi uzerine olasi etkisinden bahsetmesinden ve kopekte meme tumoru varliginda bu hastaligin goz ardi edilmemesi gerekliligini onermesinden dolayi onemlidir.

Unilateral mastectomy as an alternative treatment for gangrenous mastitis in a Saanen goat

Abstract A four-year-old Saanen goat, weighing 52 kg, was referred to the Department of Obstetrics and Gynaecology clinic with black discoloration of the left mammary gland. The goat’s general condition was not good upon initial examination. The goat was anorexic, lethargic, and febrile (rectal temperature of 40 °C) with a palpably cold left mammary gland on which there was an accumulation of pus. Somatic cell count (SCC) was measured as 820,000/ml in the milk sample collected from the infected gland, and Staphylococcus aureus was isolated. Gangrenous mastitis was diagnosed on the left mammary gland of the goat based on the clinical findings. To treat the condition, a unilateral mastectomy was performed successfully. The goat was fully recovered a week after surgery. This case aimed to establish a surgical approach of unilateral mastectomy as a treatment option for goats with gangrenous mastitis while allowing the other mammary gland to continue lactation. Thus, it can be concluded that unilateral mastectomy is a viable alternative treatment of goats with gangrenous mastitis.

The effect of oviductal cells on in vitro maturation of canine oocytes in different culture media

Sinem Özlem ENGİNLER*, Asiye İzem SANDAL, Özen Banu ÖZDAŞ, Ramazan ARICI, Ezgi ERTÜRK, Elif Merve ÇINAR, Israa Faris MOHAMMED, Alper BARAN, Çağatay TEK, Mehmet Can GÜNDÜZ Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Istanbul University, Avcılar, İstanbul, Turkey Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, Avcılar, İstanbul, Turkey