Asma Rahman

An In Vitro Study of Binding of Aceclofenac and Pantoprazole with Bovine Serum Albumin by UV Spectroscopic Method

An In Vitro Study of Binding of Aceclofenac and Pantoprazole with Bovine Serum Albumin by UV Spectroscopic Method Plasma protein binding is one of the most important pharmacokinetic parameter of drug. The study was designed to determine the binding of Aceclofenac and Pantoprazole to Bovine Serum Albumin. The study was conducted by equilibrium dialysis method followed by measurement using a spectrophotometer at pH 7.4 and 37°C

A Study of Method Development, Validation, and Forced Degradation for Simultaneous Quantification of Paracetamol and Ibuprofen in Pharmaceutical Dosage Form by RP-HPLC Method

A rapid and stability-indicating reversed phase high-performance liquid chromatography (RP-HPLC) method was developed for simultaneous quantification of paracetamol and ibuprofen in their combined dosage form especially to get some more advantages over other methods already developed for this combination. The method was validated according to United States Pharmacopeia (USP) guideline with respect to accuracy, precision, specificity, linearity, solution stability, robustness, sensitivity, and system suitability. Forced degradation study was validated according to International Conference on Harmonisation (ICH). For this, an isocratic condition of mobile phase comprising phosphate buffer (pH 6.8) and acetonitrile in a ratio of 65:35, v/v at a flow rate of 0.7 mL/minute over RP C18 (octadecylsilane (ODS), 150 × 4.6 mm, 5 μm, Phenomenex Inc.) column at ambient temperature was maintained. The method showed excellent linear response with correlation coefficient (R2) values of 0.999 and 1.0 for paracetamol and ibuprofen respectively, which were within the limit of correlation coefficient (R2 > 0.995). The percent recoveries for two drugs were found within the acceptance limit of (97.0–103.0%). Intra-and inter-day precision studies of the new method were less than the maximum allowable limit percentage of relative standard deviation (%RSD) ≤ 2.0. Forced degradation of the drug product was carried out as per the ICH guidelines with a view to establishing the stability-indicating property of this method and providing useful information about the degradation pathways, degradation products, and how the quality of a drug substance and drug product changes with time under the influence of various stressing conditions. The degradation of ibuprofen was within the limit (5–20%, according to the guideline of ICH), while paracetamol showed <20% degradation in oxidation and basic condition.

A Study of Prophylactic Effect Against Diabetes of Two Ayurvedic Drugs ‘Jambadyarista’ and ‘Bohumutrantak Ras’ in Normal as well as Alloxan-induced Diabetic Rats

Aims: The present study was designed to evaluate the anti -diabetic as well as prophylactic activity of Jambadyarista and Bohumutrantak Ras in alloxan -induced diabetic rats. Study Design:Study the prophylactic and antiglycemic effects against diabetes of two

In vitro and In vivo Studies of Drug-Drug Interaction between Metformin and Cefepime

A diabetic individual gradually becomes more prone to infections and can be prescribed Metformin concurrently with a broad spectrum antibiotic like Cefepime. Our research examines the possibility of drug-drug interaction between Metformin and Cefepime in a number of in vitro and in vivo parameters. The in vitro tests including Differential Scanning Calorimeter, Scanning Electron Microscope and Fourier Transform Infra Red analysis were found to reveal visible changes in melting points, morphological structures and rearrangement of functional groups due to the interaction. The disc diffusion method was used to compare the antimicrobial properties of all the test samples and the antimicrobial potential of Cefepime was found to be suppressed moderately after in vitro interaction with Metformin. The alteration of the in vivo antidiabetic activity of Metformin was evaluated in streptozotocin-induced Long-Evans Rats, and the anti-hyperglycemic effect of Metformin was detected to decrease significantly in the resulting product of this interaction.

Anticholinesterase and Antioxidant Potentials of a Medicinal Plant Abroma augusta: Implications for the Alternative Treatment Therapy of Cognitive Deficits in Alzheimers disease

Oxidative stress and low level of neurotransmitter (especially acetylcholine) are main characteristics of Alzheimer’s disease (AD), a progressive neurodegenerative disease. Prolonging the function of acetylcholine by inhibiting acetylcholinesterase or butyrylcholinesterase enzyme and reducing oxidative stress with antioxidants are most effective treatment therapy of AD. Abroma augusta is a well-known medicine plant with a variety of medicinal uses. In this study we examine cholinesterase inhibitory activity as well as antioxidant activity of dried fruit extract including crude methyl extract and its sub fractions (Chloroform fraction, Petroleum ether fraction, Ethyl acetate fraction and aqueous fraction) of A. augusta. Both cholinergic inhibitory activity and antioxidant activity of various fractions suggested that, ethyl acetate fraction are most prominent among all fractions and can be used in symptomatic treatment of AD.

The Pharmacokinetic Study of Aspirin, Paracetamol and Naproxen with Magnesium Sulfate

The present study was designed to evaluate the bioavailability of single drug as well as drug with Mg(II) complexes in the systemic circulation of rats. 132 healthy rats were selected for this study. The rats were fasted for 12 hours (overnight) prior to drug administration and kept fasting up to blood collection after administration of the drugs. The rats were divided in to three groups for each drug of study: control (rats without giving any drugs for each analysis) and group 1 for reference (single drug) and group 2 for test drug i.e. drug-Mg complexes. In this study both the test (drug-Mg complex) drug and corresponding reference drug were administered at the dose of aspirin at 10 mg/kg body weight, paracetamol at 16 mg/kg body weight and naproxen at 16 mg/kg body weight in the solution form through oral route. From the pharmacokinetic study, it was found that concomitant administration of magnesium with aspirin slightly increased elimination rate and lowered the bioavailability than the reference aspirin. When magnesiun was concomitantly administered with paracetamol, it markedly increased elimination rate and decreased the bioavailability than that of the reference paracetamol. When magnesiun was concomitantly administered with naproxen, it decreased elimination rate and remained in the systemic circulation for longer time.

Incompatibility of Bisoprolol Fumarate with Some Super-disintegrating Agents

Aims: Bisoprolol fumarate, a selective β1adrenoreceptor blocker, is usually formulated as immediate release tablet dosage form. While developing the immediate release tablet formula in laboratory, the assay and dissolution results were found below acceptance limit in some formulation. The formulations differed only in disintegrating agents. Therefore a chemical interaction was suspected with some of the disintegrants with the drug used in the formulas. The aims of this study were to find out the interaction with the specific excipient. Study Design: Consequently, a pilot study of binary mixture of Bisoprolol-excipients (conventionally used in solid dosage form, e.g. binder, diluents, disintegrating agents, glidants, dissolution enhancer etc.) was carried out in laboratory using different analytical methods such as dissolution tester, UV, HPLC, DSC etc. Also formulated tablets were studied. Original Research Article Shakar et al.; BJPR, 5(2): 137-145, 2015; Article no.BJPR.2015.013 138 Place: Study was carried in drug testing and analytical research laboratory in Center for Advanced Research in Sciences, University of Dhaka. Results: From the study, bisoprolol fumarate was found quite incompatible with ‘sodium starch glycolate’(SSG) and ‘croscarmellose sodium’(CCS) both of which are used as disintegrating agents in conventional solid dosage forms. But other disintegrating agents such as kollidon CL (KCL) has shown no interaction towards bisoprolol fumarate. Conclusion: Thus from this study we reached a valuable conclusion that bisoprolol fumarate is quite incompatible with two disintegrating agents namely sodium starch glycolate and croscarmellose sodium. With sodium starch glycolate, the drug was found to be degraded by around 19% whereas with croscarmellose sodium degradation was estimated around 13% in freshly prepared tablets. On the other hand, kollidon CL is compatible with this drug in its solid dosage formulation.

Development and Validation of a RP-HPLC Method for Simultaneous Determination of Levofloxacin and Moxifloxacin in Pharmaceutical Dosage Forms

A simple, fast and economic reversed phase high performance liquid chromatographic (HPLC) method has been successfully developed and validated for simultaneous determination of fluoroquinolone analogs namely levofloxacin and moxifloxacin in both pure form (as API) and in pharmaceutical dosage forms. The method was validated according to the guidelines of ICH, FDA and USP with respect to accuracy, precision and linearity. For method development a C-18 bonded silica column (250 x 4.6 mm, 5μ, Phenomenex, Inc) was used with a mobile phase comprising of 10% aqueous solution of acetic acid and acetonitrile in a ratio of 80:20 v/v. The flow rate was 0.5 mL/min and effluents were monitored at 300 nm and the retention times were found to be at 7.0±0.1 min and 10.59±0.1 min for levofloxacin and moxifloxacin, respectively. The recovery was found to be more than 99% for each spiked samples of levofloxacin and moxifloxacin, demonstrating the accuracy of the protocol. Intra-day and inter-day precisions of the new method were less than the maximum allowable limit (RSD% £ 2.0) according to FDA. The method showed linear response with correlation coefficient value of 0.9975 in both the cases. Therefore, the developed method was found to be simpler, accurate, reproducible, efficient and less time consuming and can be successfully applied for the simultaneous assay of levofloxacin and moxifloxacin formulations.