Zakir Sultan

In vitro and In vivo Studies of Drug-Drug Interaction between Metformin and Cefepime

A diabetic individual gradually becomes more prone to infections and can be prescribed Metformin concurrently with a broad spectrum antibiotic like Cefepime. Our research examines the possibility of drug-drug interaction between Metformin and Cefepime in a number of in vitro and in vivo parameters. The in vitro tests including Differential Scanning Calorimeter, Scanning Electron Microscope and Fourier Transform Infra Red analysis were found to reveal visible changes in melting points, morphological structures and rearrangement of functional groups due to the interaction. The disc diffusion method was used to compare the antimicrobial properties of all the test samples and the antimicrobial potential of Cefepime was found to be suppressed moderately after in vitro interaction with Metformin. The alteration of the in vivo antidiabetic activity of Metformin was evaluated in streptozotocin-induced Long-Evans Rats, and the anti-hyperglycemic effect of Metformin was detected to decrease significantly in the resulting product of this interaction.

The Pharmacokinetic Study of Aspirin, Paracetamol and Naproxen with Magnesium Sulfate

The present study was designed to evaluate the bioavailability of single drug as well as drug with Mg(II) complexes in the systemic circulation of rats. 132 healthy rats were selected for this study. The rats were fasted for 12 hours (overnight) prior to drug administration and kept fasting up to blood collection after administration of the drugs. The rats were divided in to three groups for each drug of study: control (rats without giving any drugs for each analysis) and group 1 for reference (single drug) and group 2 for test drug i.e. drug-Mg complexes. In this study both the test (drug-Mg complex) drug and corresponding reference drug were administered at the dose of aspirin at 10 mg/kg body weight, paracetamol at 16 mg/kg body weight and naproxen at 16 mg/kg body weight in the solution form through oral route. From the pharmacokinetic study, it was found that concomitant administration of magnesium with aspirin slightly increased elimination rate and lowered the bioavailability than the reference aspirin. When magnesiun was concomitantly administered with paracetamol, it markedly increased elimination rate and decreased the bioavailability than that of the reference paracetamol. When magnesiun was concomitantly administered with naproxen, it decreased elimination rate and remained in the systemic circulation for longer time.