Atsuki Fukutani

Nonuniform Muscle Hypertrophy: Its Relation to Muscle Activation in Training Session

PURPOSE Muscle hypertrophy in response to resistance training has been reported to occur nonuniformly along the length of the muscle. The purpose of the present study was to examine whether the regional difference in muscle hypertrophy induced by a training intervention corresponds to the regional difference in muscle activation in the training session. METHODS Twelve young men participated in a training intervention program for the elbow extensors with a multijoint resistance exercise for 12 wk (3 d · wk(-1)). Before and after the intervention, cross-sectional areas of the triceps brachii along its length were measured with magnetic resonance images. A series of transverse relaxation time (T2)-weighted magnetic resonance images was recorded before and immediately after the first session of training intervention. The T2 was calculated for each pixel within the triceps brachii. In the images recorded after the session, the number of pixels with a T2 greater than the threshold (mean + 1 SD of T2 before the session) was expressed as the ratio to the whole number of pixels within the muscle and used as an index of muscle activation (percent activated area). RESULTS The percent activated area of the triceps brachii in the first session was significantly higher in the middle regions than that in the most proximal region. Similarly, the relative change in cross-sectional area induced by the training intervention was also significantly greater in the middle regions than the most proximal region. CONCLUSION The results suggest that nonuniform muscle hypertrophy after training intervention is due to the region-specific muscle activation during the training session.

Effect of conditioning contraction intensity on postactivation potentiation is muscle dependent

We aimed to examine whether the influence of conditioning contraction intensity on the extent of postactivation potentiation (PAP) is muscle dependent. Eleven healthy males performed both thumb adduction and plantar flexion as a conditioning contraction. The conditioning contraction intensities were set at 20%, 40%, 60%, 80%, or 100% of the maximal voluntary isometric contraction (MVC). Before and after the conditioning contraction, twitch torque was measured for the respective joint to calculate the extent of PAP. In plantar flexion, the extent of PAP became significantly larger as the conditioning contraction intensity increased up to 80% MVC (p<0.05). In contrast, the extent of PAP in thumb adduction increased significantly only up to 60% MVC (p<0.05), but not at higher intensities. These results indicate that the influence of the conditioning contraction intensity on the extent of PAP is muscle dependent. Our results suggest that a conditioning contraction with submaximal intensity can sufficiently evoke sizable PAP in the muscle where most of muscle fibers are recruited at submaximal intensities, thereby attenuating muscle fatigue induced by the conditioning contraction.

Potentiation of isokinetic torque is velocity- dependent following an isometric conditioning contraction

Not only twitch torque but also the maximal voluntary concentric torque increases after a high-intensity contraction (conditioning contraction). The purpose of this study was to test the hypothesis that the increase in the maximal voluntary concentric torque induced by a conditioning contraction is prominent when tested at fast angular velocities conditions. Twelve healthy male participants performed the maximal voluntary isometric plantar flexion for six seconds as a conditioning contraction. Before and after the conditioning contraction, peak torques during the maximal voluntary concentric plantar flexions were measured at 30°/s (slow) and 180°/s (fast), each of which was carried out in a separate condition. Isometric twitch torque was also recorded before and after the conditioning contraction in each of the two velocity conditions to confirm the extent of the positive effect of the conditioning contraction. The extent of increase in isometric twitch torque was similar between the two velocity conditions, whereas the maximal voluntary concentric torque increased significantly only in the fast velocity condition (p = 0.003). These results support the hypothesis and indicate that the maximal voluntary concentric torque can be potentiated by the conditioning contraction if the joint angular velocity during the maximal voluntary concentric contraction is sufficiently high.

Factors of force potentiation induced by stretch-shortening cycle in plantarflexors.

Muscle force is potentiated by countermovement; this phenomenon is called stretch-shortening cycle (SSC) effect. In this study, we examined the factors strongly related to SSC effect in vivo, focusing on tendon elongation, preactivation, and residual force enhancement. Twelve healthy men participated in this study. Ankle joint angle was passively moved by a dynamometer, with a range of motion from 15° dorsiflexion (DF) to 15° plantarflexion (PF). Muscle contraction was evoked by electrical stimulation, with stimulation timing adjusted to elicit three types of contraction: (1) concentric contraction without preliminary contraction (CON), (2) concentric contraction after preliminary eccentric contraction (ECC), and (3) concentric contraction after preliminary isometric contraction (ISO). Joint torque was recorded at DF5°, PF0°, and PF5°, respectively. SSC effect was calculated as the ratio of joint torque obtained in ECC or ISO with respect to that obtained in CON at the aforementioned three joint angles. SSC effect was prominent in the first half of movement in both ECC (DF5°, 329.3 ± 101.2%; PF0°, 159.2 ± 29.4%; PF5°, 125.5 ± 20.8%) and ISO (DF5°, 276.4 ± 87.0%; PF0°, 134.5 ± 24.5%; PF5°, 106.8 ± 18.0%) conditions. SSC effect was significantly larger in ECC than in ISO at all joint angles (P < 0.001). Even without preliminary eccentric contraction (i.e., ISO condition), SSC effect was clearly large, indicating that a significant part of SSC effect is derived from preactivation. However, the active lengthening-induced force potentiation mechanism (residual force enhancement) also contributes to SSC effect.

Relationship between joint torque and muscle fascicle shortening at various joint angles and intensities in the plantar flexors

Because it is difficult to measure tendon length changes directly in humans, tendon length changes during dynamic movement have been evaluated indirectly from changes in muscle fascicle length and joint angle. The purpose of this study was to examine the validity of the indirect method. Twitch contractions of the ankle plantar flexors were evoked isometrically in eight subjects. Twitch contractions evoked by singlet, doublet, and triplet stimulations were conducted at dorsiflexion 20° (DF20), plantar flexion 0° (PF0), and plantar flexion 20° (PF20). Muscle fascicle length and pennation angle were recorded by ultrasonography. The magnitude of muscle fascicle shortening was significantly smaller in DF20 than in PF0 and PF20, although the magnitude of joint torque was significantly larger in DF20 than in PF0 and PF20. Theoretically, the magnitude of tendon elongation is expected to be larger in larger joint torque conditions. However, we found that the magnitude of tendon elongation evaluated from muscle fascicle shortening was larger in a lower joint torque condition (PF20). These results suggest that the magnitude of muscle fascicle shortening does not necessarily represent tendon elongation. The larger muscle fascicle shortening in PF20 may be partly caused by eliminating slack of the muscle-tendon complex.

Tendon Cross-Sectional Area Is Not Associated with Muscle Volume

Recent studies have reported that resistance training increases the cross-sectional areas (CSAs) of tendons; however, this finding has not been consistently observed across different studies. If tendon CSA increases through resistance training, resistance-trained individuals should have larger tendon CSAs as compared with untrained individuals. Therefore, in the current study, we aimed to investigate whether resistance training increases tendon CSAs by comparing resistance-trained and untrained individuals. Sixteen males, who were either body builders or rugby players, were recruited as the training group, and 11 males, who did not participate in regular resistance training, were recruited into the control group. Tendon CSAs and muscle volumes of the triceps brachii, quadriceps femoris, and triceps surae were calculated from images obtained by using magnetic resonance imaging. The volumes of the 3 muscles were significantly higher in the training group than in the control group (P < .001 for all muscles). However, a significant difference in tendon CSAs was found only for the distal portion of the triceps surae tendon (P = .041). These findings indicate that tendon CSA is not associated with muscle volume, suggesting that resistance training does not increase tendon CSA.

Influence of neglecting the curved path of the Achilles tendon on Achilles tendon length change at various ranges of motion

Achilles tendon length has been measured using a straight‐line model. However, this model is associated with a greater measurement error compared with a curved‐line model. Therefore, we examined the influence of neglecting the curved path of the Achilles tendon on its length change at various ranges of motion. Ten male subjects participated in this study. First, the location of the Achilles tendon was confirmed by using ultrasonography, and markers were attached on the skin over the Achilles tendon path. Then, the three‐dimensional coordinates of each marker at dorsiflexion (DF) 15°, plantarflexion (PF) 0°, PF15°, and PF30° were obtained. Achilles tendon length in the curved‐line model was calculated as the sum of the distances among each marker. On the other hand, Achilles tendon length in the straight‐line model was calculated as the straight distance between the two most proximal and distal markers projected onto the sagittal plane. The difference of the Achilles tendon length change between curved‐line and straight‐line models was calculated by subtracting the Achilles tendon length change obtained in curved‐line model from that obtained in straight‐line model with three different ranges of motion (i.e., PF0°, PF15°, and PF30° from DF15°, respectively). As a result, the difference in Achilles tendon length change between the two models increased significantly as the range of motion increased. In conclusion, neglecting the curved path of the Achilles tendon induces substantial overestimation of its length change when the extent of ankle joint angle change is large.

Effect of Preactivation on Torque Enhancement by the Stretch-Shortening Cycle in Knee Extensors.

The stretch-shortening cycle is one of the most interesting topics in the field of sport sciences, because the performance of human movement is enhanced by the stretch-shortening cycle (eccentric contraction). The purpose of the present study was to examine whether the influence of preactivation on the torque enhancement by stretch-shortening cycle in knee extensors. Twelve men participated in this study. The following three conditions were conducted for knee extensors: (1) concentric contraction without preactivation (CON), (2) concentric contraction with eccentric preactivation (ECC), and (3) concentric contraction with isometric preactivation (ISO). Muscle contractions were evoked by electrical stimulation to discard the influence of neural activity. The range of motion of the knee joint was set from 80 to 140 degrees (full extension = 180 degrees). Angular velocities of the concentric and eccentric contractions were set at 180 and 90 degrees/s, respectively. In the concentric contraction phase, joint torques were recorded at 85, 95, and 105 degrees, and they were compared among the three conditions. In the early phase (85 degrees) of concentric contraction, the joint torque was larger in the ECC and ISO conditions than in the CON condition. However, these clear differences disappeared in the later phase (105 degrees) of concentric contraction. The results showed that joint torque was clearly different among the three conditions in the early phase whereas this difference disappeared in the later phase. Thus, preactivation, which is prominent in the early phase of contractions, plays an important role in torque enhancement by the stretch-shortening cycle in knee extensors.

Comparison of the Achilles tendon moment arms determined using the tendon excursion and three‐dimensional methods

The moment arm of muscle‐tendon force is a key parameter for calculating muscle and tendon properties. The tendon excursion method was used for determining the Achilles tendon moment arm (ATMA). However, the accuracy of this method remains unclear. This study aimed to investigate the magnitude of error introduced in determining the ATMA using the tendon excursion method by comparing it with the reference three‐dimensional (3D) method. The tendon excursion method determined the ATMA as the ratio between the Achilles tendon displacement during foot rotation from 15° of dorsiflexion to 15° of plantarflexion and the joint rotation angle. A series of foot images was obtained at 15° of dorsiflexion, the neutral position, and 15° of plantarflexion. The 3D value of the ATMA was determined as the shortest distance between the talocrural joint axis and the line of action of the Achilles tendon force. The ATMA determined by the tendon excursion method was smaller by 3.8 mm than that determined using the 3D method. This error may be explained mainly by the length change in the Achilles tendon due to the change in the force applied to it, as passive plantarflexion torque was different by 11 Nm between 15° of dorsiflexion and 15° of plantarflexion. Furthermore, the ATMAs determined using the 3D and tendon excursion methods were significantly correlated but the coefficient of determination was not large (R2 = 0.352). This result suggests that the tendon excursion method may not be feasible to evaluate the individual variability of the ATMA.

Can a High-Intensity Contraction Be Enhanced by a Conditioning Contraction? Insight from the Relationship Between Shortening Velocity of Muscle Fibers and Postactivation Potentiation

The magnitude of twitch torque increases after a high-intensity contraction of the same muscle (conditioning contraction). This phenomenon is called postactivation potentiation (PAP). Recently, it has been shown that the maximal voluntary concentric torque or power attained during the maximal voluntary concentric contraction can be increased by a conditioning contraction, suggesting that conditioning contractions are effective on not only twitch but also on maximal voluntary contractions. In contrast, some studies have reported that a conditioning contraction had no potentiation effect on subsequent electrically-evoked maximal isometric force. This discrepancy among previous studies may be attributable to differences in the mode of contraction (i.e., isometric, concentric or eccentric), which can affect the extent of PAP. Therefore, the main purpose of this study was to examine the influence of these aforementioned factors on the extent of PAP, and to discuss the applications of a conditioning contraction to high-intensity contractions.