Azize Alayli Gungor

Removing Trypan blue dye using nano-Zn modified Luffa sponge.

This study has presented specific features that are examined to remove the Trypan blue dye from the waste using Luffa sponge (LS) and modified Luffa sponge with zinc nanoparticles (ZnNPs). Peroxidase enzyme was obtained from Euphorbia amygdaloides plant and it was used with the green synthesis of Zn nanoparticles. Luffa sponge was used to be a support material for immobilized nanoparticles and it also used in remediation work. The obtained membrane forms, fibrous materials, (LS, ZnNPs-LS) were characterized with SEM and XRD. LS and ZnNPs-LS were employed as adsorbent to be used for the removal of Trypan blue dye from aqueous via batch studies. Measurements were made for the equilibrium, pH, temperature, concentration of dye with UV-visible spectrometer (590nm; for Trypan blue dye). The optimum removal of Trypan blue dye was found at pH7, the equilibrium was attained within 30min. The thermodynamic properties ΔG0, ΔH0, and ΔS0 showed that adsorption of Trypan blue dye onto LS and ZnNPs-LS were spontaneous and endothermic. The equilibrium isotherm data were analyzed using Langmuir and Freundlich models and the sorption process was described by the Langmuir isotherm with maximum monolayer adsorption capacity of 45.32 and 47.3mg/g for LS and LS-ZnNPs at 303±1°K, respectively.

Green synthesis and characterisation of platinum nanoparticles using quail egg yolk.

Nanotechnology is extensively used in all parts today. Therefore, nano synthesis is also significant in all explored areas. The results of studies conducted have revealed that nanoparticle synthesis is performed by using both chemical and physical methods. It is well known that these syntheses are carried out at high charge, pressure and temperature in harsh environments. Therefore, this study investigated green synthesis method that sustains more mild conditions. In this study, quail egg yolk having high vitamin and protein content was prepared for green synthesis reaction and used for the synthesis of platinum nanoparticles in the reaction medium. Reaction situations were optimised as a function of pH, temperature, time and concentration by using quail egg yolk. The results showed that the highest platinum nanoparticles were synthesised at 20°C and pH6.0 for 4h. Also, optimal concentration of metal ions was established as 0.5mM. The synthesised platinum nanoparticles were characterised by using UV spectrum, X-ray diffraction and scanning electron microscope.

Biochemical Evaluation and Green Synthesis of Nano Silver Using Peroxidase from Euphorbia (Euphorbia amygdaloides) and Its Antibacterial Activity

Silver nanoparticles are used an increased attention for various biomedical and medical applications. In this study, green synthesis of silver nanoparticles was made with simple method by using peroxidase enzyme partially purified from Euphorbia (Euphorbia amygdaloides) plant. Optimum pH, temperature and time period were determined to obtain silver nanoparticles using the peroxidase enzyme. The result shows that higher silver nanoparticle was synthesized for 4 hours and at 20°C and pH 8. Also, optimal concentration of metal ions was found as 0.5 mM. The synthesized silver nanoparticles were characterized by UV spectrum, scanning electron microscope (SEM) and X-ray diffraction. Antibacterial activity of silver nanoparticles was measured against some microorganisms such as Serratia marcescens, Yersinia pseudotuberculosis, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, and Escherichia coli. Synthesized silver nanoparticles have wide spectrum antibacterial activity in low concentration and may be a good alternative therapeutic approach in medicine and pharmaceutical fields in future.

Green synthesis of γ-Fe 2 O 3 nanoparticles for methane gas sensing

Green synthesis is becoming increasingly important as an eco-friendly alternative to the traditional production process because of its growing industrial applications. In this study, peroxidase enzymes of the leaf extracts of the plants Ficus carica (Fig) and Euphorbia amygdaloides (Euphorbia) were used for the synthesis of γ-Fe2O3 nanoparticles (NPs). The structural, morphological and methane gas sensing properties of γ-Fe2O3 NPs were investigated. γ-Fe2O3 NPs obtained from the Fig plant peroxidase enzyme showed a higher response, selectivity and short response/recovery time than γ-Fe2O3 NPs obtained from the Euphorbia plant peroxidase enzyme, which exhibited 15% response to 1-ppm methane gas at 150 °C. Impedance spectroscopy analysis showed that the resistance due to the grain boundaries significantly contributed to the characteristics of gas sensing. It can be seen from results that γ-Fe2O3 NPs produced from peroxidase enzymes that purified from Fig plant have great potential for industrial applications.

Green synthesis of gold nanoparticles using quail egg yolk and investigation of potential application areas

Abstract Nanotechnology is a growing area of research. For example, gold nanoparticles have a wide variety of applications, including optical, electronic, and medical; they are also used as catalysts in biosensor applications and can be used for scanning, carrier, heat source, and sensors. Therefore, nanoparticle synthesis is of great importance to the country’s economy and to scientific development. Nanoparticle synthesis involves using chemical methods, physical methods, or both chemical and physical methods. The synthesis is performed at high pressures or high temperatures and severe conditions, both of which have a high cost in terms of energy. In our investigation, we used a green synthesis method, which used quail eggs that required more moderate conditions and less energy. The yolk from quail eggs has a high protein and vitamin content. Using quail egg yolks, the reaction conditions were optimized in terms of pH, temperature, and concentration. The morphological properties of the obtained gold nanoparticles were characterized using an ultraviolet-visible spectrophotometer, a scanning electron microscope, and an X-ray diffraction analysis.

An Enzymatic Method for Zinc Determination in Wastewater

Aims: In this study, an enzymatic zinc determination method was based on the r egaining of the activity of apo carbonic anhydrase by the zinc is presented in the sample. Study Design:This method determines the amount ofzincin the waste waterofthe environment canbe used in orderto find outhow much contaminated.For this purpose, wastewater from seven different areas and three drinking waters in Erzurum were taken and zinc concentrations were determined . Methodology: Carbonic anhydrase was purified by Sepharose-4B-L tyrosine sulphanilamidaffinity chromatography from bovine erythrocytes. The zinc in this structure was removed by dialysis against dipicolinic acid resulting in apo carbonic anhydrase obtained at a ratio of 100%. The activity of the enzyme was determined by the esterase activity on p -nitrophenylacetate. For comparison purposes, the same samples were analyzed in an atomic absorption (AAS). Results: When we compared the results of the AA and the enzymatic method, we Original ResearchArticle