Beom-Il Lee

Mucilaginibacter soyangensis sp. nov., isolated from a lake

A non-motile, yellow-orange-pigmented bacterial strain, designated HME6664(T), was isolated from Lake Soyang, Republic of Korea. The major fatty acids of strain HME6664(T) were summed feature 3 (comprising C16 : 1ω6c and/or C(16 : 1)ω7c; 44.7%) and iso-C15 : 0 (20.2%). The DNA G+C content was 40.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6664(T) formed a lineage within the genus Mucilaginibacter. Strain HME6664(T) was closely related to Mucilaginibacter ximonensis (96.7%), Mucilaginibacter dorajii (96.5%) and Mucilaginibacter lappiensis (96.3%). On the basis of the evidence presented in this study, strain HME6664(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter soyangensis sp. nov., is proposed. The type strain is HME6664(T) ( = KCTC 23261(T) = CECT 7824(T)).

Sediminibacterium goheungense sp. nov., isolated from a freshwater reservoir.

A novel bacterial strain designated HME7863(T) was isolated from a freshwater reservoir in Goheung, Republic of Korea. Cells of strain HME7863(T) were Gram-staining-negative, strictly aerobic, orange-pigmented, rod-shaped and motile by gliding. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain HME7863(T) formed a lineage within the genus Sediminibacterium. Strain HME7863(T) was closely related to Sediminibacterium ginsengisoli DCY13(T) (96.9 % sequence similarity) and Sediminibacterium salmoneum NJ-44(T) (96.4 %). The major cellular fatty acids were iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0 3-OH. Polar lipid analysis revealed the presence of phosphatidylethanolamine, five unidentified aminolipids, two unidentified aminophospholipids, one unidentified phospholipid and two unidentified polar lipids. The only respiratory quinone was MK-7. The DNA G+C content was 40.5 mol%. On the basis of the evidence presented in this study, strain HME7863(T) represents a novel species of the genus Sediminibacterium, for which the name Sediminibacterium goheungense sp. nov., is proposed. The type strain is HME7863(T) ( = KCTC 23945(T) = CECT 8100(T)).

Chitinimonas viridis sp. nov., isolated from a mesotrophic artificial lake.

A Gram-staining-negative, rod-shaped bacterium, strain HMD2169(T), was isolated from a mesotrophic artificial lake in Korea. Strain HMD2169(T) grew in the presence of 0-3.0% (w/v) NaCl, at pH 5-10 and at 20-37 °C. The predominant quinone of strain HMD2169(T) was ubiquinone (UQ)-8. The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 59.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD2169(T) was a representative of a lineage within the genus Chitinimonas. Strain HMD2169(T) was closely related to Chitinimonas taiwanensis (95.8 % sequence similarity) and Chitinimonas koreensis (94.6 %). On the basis of the evidence presented in this study, strain HMD2169(T) is a representative of a novel species of the genus Chitinimonas, for which the name Chitinimonas viridis sp. nov. is proposed with the type strain HMD2169(T) ( = KCTC 22839(T) = CECT 7703(T)).

Pontibacter jeungdoensis sp. nov., isolated from a solar saltern in Korea

A Gram-staining-negative, rod-shaped and red-pigmented bacterial strain, HMD3125T, was isolated from a solar saltern in Jeungdo, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD3125T formed a lineage within the genus Pontibacter and was similar to Pontibacter salisaro (96.1%) and P. korlensis (95.3%). The major fatty acids of strain HMD3125T were summed feature 4 (comprising iso-C17:1 I and/or anteiso-C17:1 B; 30.4%), iso-C15:0 (20.4%) and iso-C17:0 3OH (17.2%). The polar lipid profile of HMD3125T consisted of the phosphatidylethanolamine, four unidentified polar lipids, unidentified phospholipid, unidentified aminolipid and unidentified aminophospholipid. Strain HMD3125T contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The DNA G+C content of strain HMD3125T was 45.6 mol%. Strain HMD3125T assigned as a novel species in the genus Pontibacter, for which the name Pontibacter jeungdoensis sp. nov. is proposed. The type strain is HMD3125T (=KCTC 23156T =CECT 7710T).

Mucilaginibacter flavus sp. nov., isolated from wetland.

A non-motile, pale yellow, colony-forming strain, designated HME6839(T), was isolated from the wetland of Jeju Island, Republic of Korea. The major fatty acids of strain HME6839(T) were summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0 and C16 : 1ω5c. The DNA G+C content was 41.2 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6839(T) formed a lineage within the genus Mucilaginibacter. Strain HME6839 (T) [corrected] was closely related to Mucilaginibacter dorajii (96.7 %), Mucilaginibacter polysacchareus (96.5 %) and Mucilaginibacter lappiensis (96.3 %). On the basis of the chemotaxonomic and phylogenetic results presented in this study, strain HME6839(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter flavus sp. nov., is proposed. The type strain is HME6839(T) ( = KCTC 23441(T) = CECT 7857(T)).

Lacihabitans soyangensis gen. nov., sp. nov., a new member of the family Cytophagaceae, isolated from a freshwater reservoir.

A Gram-staining-negative, non-motile and orange-pigmented bacterium, designated strain HME6675(T), was isolated from freshwater of a reservoir in Korea. The major fatty acids of strain HME6675(T) were iso-C15 : 0 (33.4 %) and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c; 31.3 %). The major respiratory quinone was MK-7. The polar lipids were phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and three unidentified polar lipids. The DNA G+C content of strain HME6675(T) was 37.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6675(T) formed a lineage within the family Cytophagaceae and was related to Leadbetterella byssophila 4M15(T) (93.0 % sequence similarity), Fluviimonas pallidilutea TQQ6(T) (90.6 %) and Emticicia oligotrophica GPTSA100-15(T) (89.1 %). On the basis of the evidence presented in this study, strain HME6675(T) represents a novel genus and species of the family Cytophagaceae, for which the name Lacihabitans soyangensis gen. nov., sp. nov. is proposed. The type strain of Lacihabitans soyangensis is HME6675(T) ( = KCTC 23259(T) = CECT 7826(T)).

Ferruginibacter yonginensis sp. nov., isolated from a mesotrophic artificial lake.

A Gram-stain-negative, rod-shaped, aerobic and reddish-pigmented strain, designated HME8442(T), was isolated from a mesotrophic artificial lake. The strain grew optimally at 30 °C and pH 7 on R2A agar. The major fatty acid was iso-C15 : 0. The polar lipids were phosphatidylethanolamine, one unidentified aminolipid and three unidentified polar lipids. The predominant respiratory quinone was MK-7. The DNA G+C content was 35.8 mol%. Strain HME8442(T) was closely related to Ferruginibacter lapsinanis HU1-HG42(T) (94.4 % 16S rRNA gene sequence similarity) and Ferruginibacter alkalilentus HU1-GD23(T) (93.9 %). The phylogenetic tree based on 16S rRNA gene sequences showed that strain HME8442(T) formed a lineage within the genus Ferruginibacter. On the basis of the evidence presented in this study, strain HME8442(T) represents a novel species of the genus Ferruginibacter, for which the name Ferruginibacter yonginensis sp. nov. is proposed. The type strain is HME8442(T) ( = KACC 17314(T) = CECT 8289(T)).

Pedobacter soyangensis sp. nov., isolated from Lake Soyang in Korea

Strain HME6451T was isolated from Lake Soyang in Korea. Phylogenetic tree based on 16S rRNA gene sequence showed that strain HME6451T formed a lineage within the genus Pedobacter. The strain HME6451T was closely related to Pedobacter daechungensis (95.4% sequence similarity), Pedobacter lentus (94.4%), and Pedobacter glucosidilyticus (93.8%). And strain HME6451T was a Gram-staining-negative, short rod-shaped, strictly aerobic bacterium. The major fatty acids were iso-C15:0 (41.2%), summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c; 23.1%), and iso-C17:0-3OH (10.1%). The polar lipids of HME6451T were consisted of one phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and four unidentified polar lipids. The DNA G+C content was 36.0 mol%. On the basis of the evidence presented in this study, strain HME6451T represent a novel species of the genus Pedobacter, for which the name Pedobacter soyangensis sp. nov., is proposed the type strain HME6451T (=KCTC 23467T =CECT 7865T).