Bertrand Tatsinkou Fossi

Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soils

An amylolytic yeast strain was isolated from starchy soils (flour mills environment, flour market, cassava farms after harvesting and transformation of tubers) and its enzyme productivity and activity evaluated. The enzyme synthesis was optimum at 30°C when initial pH of fermentation medium was 4.5. After extraction and partial purification, the enzyme remained stable in a range of temperature and pH between 20°C-60°C and 2-8 pH respectively. The optimum enzyme activity was displayed at 70°C and pH 5.5 and 6.5. The thermostability of the enzyme was comparable to that of amylases from bacteria. Key Words: Amylolytic yeast, amylase activity, thermostable amylase. African Journal of Biotechnology Vol.4(1) 2005: 14-18

Application of Amylolytic Lactobacillus fermentum 04BBA19 in Fermentation for Simultaneous Production of Thermostable Alpha-Amylase and Lactic Acid

© 2013 Fossi and Tavea, licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Application of Amylolytic Lactobacillus fermentum 04BBA19 in Fermentation for Simultaneous Production of Thermostable -Amylase and Lactic Acid