Betty G. Uzman

Nuclear bodies: A component of cell nuclei in hamster tissues and human tumors

Nuclear bodies which are seen as 1–1.5 μ large, fibrous whorls in some cell nuclei have been recorded in the present study in cells from a number of human tumors; lymph nodes, bone marrow and spleen of patients with leukemia and Hodgkins disease; neurons in Dawsons subacute sclerosing leukoencephalitis; renal tubular cells in cytomegalic inclusion disease; and in cells from choroid plexus in two normal infants. In this study, large nuclear bodies were seen most frequently in the reticuloendothelial cells of thymus, lymph nodes, and spleen of hamsters carrying intraperitoneal transplants of human leukemic lymphoblasts. In many cases, interchromatinic granules and small masses of chromatin are seen associated with the fibrous nuclear bodies. On the basis of some preliminary histochemical staining and enzyme extractions, it appears probable that the fibrous nuclear bodies do not contain any DNA or RNA but may have proteins in their structure.

Production and secretion of chondroitin sulfates and dermatan sulfate by established mammalian cell lines

Abstract Ten established mammalian cell lines of different origin (lymphoid, epithelial and fibroblastic) were found to produce and secrete a mixture of chondroitin sulfates A, C and dermatan sulfate into the medium. The rates of production and secretion of the total sulfated mucopolysaccharides as well as the proportion of individual mucopolysaccharides differ strikingly among different cell lines.

Nature of the Packing of Ribosomes within Chromatoid Bodies

Optical Fourier transformns, made of electron mnicrographs of the crystalline array of ribonucleoprotein known as chromatoid bodies of Entamoeba invadens, have been interpreted as the transforms of a helix of 12 nodes in five turns. A model shows that this helix may be built of spheres 180 angstroms in diameter, placed at a radius of 150 angstroms. The optical transform of the model is very similar to the transform of the original electron micrograph.

Pentagonal Aggregation of Virus Particles

Virus aggregates with a unique fivefotd axis have been observed in the electron microscope.

Peripheral Nerve Regeneration in Quail-Chick Spinal Cord Chimeras Treated with Oral Ciclosporin

Regeneration of chimeric wing nerves in 2 quail-chick spinal cord chimeras is reported. The large DNA-containing quail nucleolar marker could not be distinguished in flattened cells, and in particular, in perineurial cell nuclei of chimeric nerves. With oral ciclosporin treatment, rejection of graft-derived nervous system tissues was acute and florid in one chimera, but followed a chronic, remitting course in the other.