Betul Kargul

Viability of fibroblasts in a novel probiotic storage media

A number of storage media have been investigated as to their ability to maintain the viability of the periodontal ligament (PDL) cells and thus to permit longer extra-alveolar periods prior to replantation of avulsed teeth. The aim of the present in vitro study was to evaluate the number of viable PDL cells of avulsed teeth treated by Hanks Balanced Salt Solutions (HBSS), saline, a novel probiotic solution and milk. Thirty-six freshly extracted single-rooted human teeth with closed apices were divided into one of the four experimental groups and two control groups (N = 6 each). The positive and negative controls corresponded to 0 min and an 8-h dry time respectively. Following extraction, the coronal 3 mm of PDL tissue was scraped with a #15 scalpel to remove cells that might have been damaged. The experimental teeth were dried for 30 min followed by a 45 min immersion in one of the four experimental media. Each experimental tooth, after drying and soaking, was incubated for 30 min with a 2.5 ml solution of 0.2 mg ml(-1) of collagenase CLS II and a 2.4 mg ml(-1) solution of dispase grade II in phosphate buffer saline (PBS). The cells were then labelled with 0.4% Trypan blue for determination of viability. The teeth stored in positive control demonstrated the highest number of viable PDL cells followed in rank order by HBSS, saline, Lactobacillus reuteri solution and milk. There was no significant difference in the number of viable PDL cells between HBSS, milk, L. reuteri solution and saline. Within the parameters of this study, it appears that probiotic may be able to maintain PDL cell viability as HBSS, milk, or saline.

An audit of the time to initial treatment in avulsion injuries.

UNLABELLED The prognosis of avulsed teeth depends on prompt and appropriate treatment. Good outcome requires education of the general public and non-dental professionals. AIM Retrospective observational survey of case records of avulsion injuries attending a dental hospital trauma clinic. METHOD Data collected included: hospital number, date of birth, gender, source of patients referral, date of trauma, number of avulsed teeth, place of initial presentation, storage, hours till initial presentation, and initial treatment. RESULTS One hundred and twenty teeth with avulsion were identified in 75 children. The mean age of the patients was 9.8 years (SD = 2.3 years) at the time of trauma with avulsions recorded in 44 (58.7%) boys and in 31 (41.3%) girls. Only 51 (42.5%) teeth were stored in an appropriate medium before attendance at any site and only 48 (40%) of the teeth were seen within 1 h. 83.3% received emergency treatment at general hospital, 89.7% in dental practice and 92.9% at dental hospital. CONCLUSIONS A minority of avulsion injuries were seen within the first hour and a minority were in appropriate storage medium at presentation. Geographical location plays a huge role in the time taken to reach secondary care. However, improving public and non-dental professional knowledge about tooth storage in avulsion injuries is critical to long-term prognosis of the teeth.

Biocompatibility of Accelerated Mineral Trioxide Aggregate on Stem Cells Derived from Human Dental Pulp

The aim of this study was to evaluate the effects of several additives on the setting time and cytotoxicity of accelerated-set mineral trioxide aggregate (MTA) on stem cells of human dental pulp. ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Johnson City, TN) was mixed with various additives including distilled water, 2.5% disodium hydrogen phosphate (Na2HPO4) (Merck, Darmstadt, Germany), K-Y Jelly (Johnson & Johnson, Markham, ON, Canada), and 5% and 10% calcium chloride (CaCl2) (Merck). The setting times were evaluated using a Vicat apparatus (Alsa Lab, Istanbul, Turkey). Human dental pulp stem cells were isolated and seeded into 48-well plates at 2 × 10(3) cells per well and incubated with MTA samples for 24 hours, 3 days, and 7 days. Cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. MTA mixed with 10% CaCl2 showed the lowest setting time (P < .05). According to the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium results on the 1st, 3rd, and 7th days, a statistically significant difference was found (P < .05) between MTA groups and the control group. MTA mixed with K-Y Jelly in all groups showed the lowest cell viability at all time points (P < .05). The cell viability of MTA mixed with distilled water, 5% CaCl2, 10% CaCl2, and Na2HPO4 increased significantly through time (P < .05). This in vitro study found MTA mixed with 5% and 10% CaCl2 and Na2HPO4 is biocompatible with dental pulp stem cells in terms of cell viability. Further in vitro and in vivo investigations are required to prove the clinical applications of MTA mixed with various additives.

A Quantitative Analysis of a Probiotic Storage Media for Avulsed Teeth

AIM The aim of the present in vitro study was to investigate the potential of a storage medium, probiotic yogurt (Bifidibacterium animalis DN 173010) in comparison with Hanks balanced salt solution (HBSS), saline and milk in maintaining viable periodontal ligament (PDL) cells on simulated avulsed teeth. MATERIALS AND METHODS Thirty-six freshly extracted single-rooted human teeth with closed apices were divided into six experimental groups (N=6). The teeth were extracted as atraumatically as possible and washed in sterile saline solution to eliminate residual blood. Following extractions, the coronal 3 mm of PDL tissues were scraped with a #15 scalpel to remove cells that may have been damaged. The positive and negative controls corresponded to 0 minutes and an 8-hour dry time, respectively. After extraction, the positive control teeth were immediately treated with dispase and collagenase. The negative control teeth were bench-dried for 8 h, with no follow-up storage solution time, and then placed in the dispase and collagenase. The number of viable protective least significant difference PDL cells were counted under a light microscope with a hemocytometer at 20× magnification and analyzed. Statistical analysis of the data was accomplished using Nonparametric ANOVA complemented by Kruskal-Wallis Test and Dunns Multiple Comparisons Test. RESULTS Positive control was found to be significantly better than the others, there were statistically significant differences between positive control and other test groups (p=0.000). The teeth stored in positive control demonstrated the highest number of viable PDL cells followed in order by probiotic yogurt, HBSS, saline and milk. CONCLUSION Bifidibacterium animalis DN 173010 seems to be an alternative for the temporary storage of avulsed teeth, due to high number of viable PDL cells. Probiotics may be suitable transport media for avulsed teeth, but further research is warranted using the commercially available products.

Possible Medical Aetiological Factors and Characteristics of Molar Incisor Hypomineralisation in a Group of Turkish Children

Libraries have an exceptional place in the history, culture, education and scientific life of a nation. They collect all aspects of our linguistics and literacy, all out theoretical assumptions as well as all the results of experience and practice. The importance of a library is not mirrored only in the national and historical role and heritage, but in a more permanent, informational role, since a modern library must, above all, be an effective information system. Since a library of a university operates as a part of its matrix, it is easily shadowed by other forms of educational and scientific infrastructure. 50(th) anniversary of the Central Dental Library of the School of Dental Medicine University of Zagreb is an excellent opportunity to make a call to the institution and public to its unique and irreplaceable role.Sažetak Svrha:�Željele su se odrediti klinicke znacajke i moguci medicinski uzroci molarno incizivne hipominerelizacije (MIH-a) u skupini turske djece. Materijali i metode: U ispitivanju je sudjelovalo 228 djece u dobi od 7 do 14 godina. Svi su pregledani na Odjelu za djecju dentalnu medicinu Stomatoloskog fakulteta Sveucilista Marmara u Istambulu. Prenatalni i postnatalni podatci prikupljeni su popunjavanjem upitnika. Rezultati: MIH je pronađen kod 54 (24 %) djeteta, a 41 (76 %) imalo je samo ogranicena podrucja opaciteta (1. stupanj). Kod osam (15%) djevojcica i djecaka bile su obavljene atipicne restauracije (2. stupanj), a barem jedno posteruptivno ljustenje (3. stupanj) uoceno je kod njih pet (9 %). MIH je svima uglavnom zahvacao kutnjake (55 %), a ne sjekutice (45 %; p<0,001). U skupini s MIH-om bili su cesti prenatalni bronhitis i hipertenzija. Nije bilo vece razlike u medicinskoj anamnezi do dobi od tri godine između skupine s MIH-om i skupine bez njega, osim duljeg uzimanja lijekova i astme u skupini s molarno incizalnom hipomineralizacijom (p<0,05). Zakljucak:�Premda etiologija MIH-a ostaje nejasna, cest je kod djece koja neprekidno uzimaju lijekove i oboljela su od astme u prve tri godine života.

Oral health and related factors in a group of children with cystic fibrosis in Istanbul, Turkey

BACKGROUND Cystic fibrosis (CF) patients can be considered as high caries risk patients because they frequently consume sugar-rich food between meals and they have a high intake of sugar containing syrups, aerosols, and salivary flow reducing medication. Variable caries prevalences were reported in CF patients in previous studies. There are no studies related to CF and salivary thromboplastic activity, which can be presented as a marker of wound healing and bleeding tendency of oral cavity. OBJECTIVE The aim of this study was to compare oral health status and salivary pH, flow rate, and thromboplastic activity in children with CF and healthy controls. MATERIALS AND METHODS A sample of 35 children with CF (23 girls and 12 boys), and 12 healthy control subjects (6 girls and 6 boys) were selected. Caries experience, oral hygiene, and dental erosion were assessed. Salivary flow rate, pH, thromboplastic activity, and total protein content were determined. Differences between the groups were evaluated using Chi-square test with a significance level set at 0.05. RESULTS The differences between children with CF and healthy controls in tooth brushing frequency, use of fluoride tablets, caries experience, dental erosion index, oral hygiene index, salivary flow rate and total protein levels were not statistically significant (P > 0.05). Salivary thromboplastic activity of the CF group was significantly lower than the healthy controls (P < 0.01). CONCLUSION Large population studies may be necessary to establish the role of salivary thromboplastic activity in children with CF considering our findings related to the decreased salivary thromboplastic activity, which may indicate delayed oral wound healing process.

Conventional endodontic treatment of primary molars using metronidazole as an intra-canal medicament: a pilot study

AIM: This was to evaluate the clinical and radiographic outcomes of an antibacterial drug (Metronidazole, Nidazol, IE Ulagay Ilac A.S) application as an intra-canal medicament combined with pulpectomy in infected primary molar teeth. METHODS: The study material consisted of data collected from children treated at the Dental School Dept. of Paediatric Dentistry in Marmara University between 2000 and 2004. Clinical and radiographic data were collected over 2 years from patients who had received a topical application of metronidazole in root canal dressing before a pulpectomy was completed. Clinical success parameters were: no abscess formation, no fistula, no pain and no pathologic mobility at treated teeth with metranidazole dressing. The overall success and failure rates were analysed. Radiographic diagnosis was standardized between investigators and intra and inter-rater reliability assessed. Both investigators read and evaluated all radiographs, after a comparison of results, a consensus was agreed upon for each result. STATISTICS: All data were entered into an Excel format and SPSS 11.0 P < 0.05 were used for Windows and Chi-square for statistical analyses. RESULTS: There were 64 molars assessed for clinical and radiographic success. Considering the eruption times, success rate was 75% as determined by the last follow up clinically and radiographically according to predetermined success criteria. In the 64 molars, 4 cases demonstrated loss of the alveolar bone, 3 exhibited varying degrees of root resorptions on radiographic examination and 3 showed clinical pathologic mobility. Fistulae were observed in only 1 case and early loss was detected in 5 cases. CONCLUSION: These results suggest that main factors responsible for failure may be associated with uncertain mixing proportions of the metronidazole paste and inadequate maxillary restorations. But some modifications in preparing the paste could increase its efficacy.

A novel quantitative light‑induced fluorescence device for monitoring molar‑incisor hypomineralization

Background: The FluoreCam system is based on an innovative approach to the quantification of enamel health termed fluorescence enamel imaging (FEI). Enamel is both highly mineralized and semi-translucent. Because of its mineral composition, enamel will fluoresce when exposed to certain light wavelengths. The semi-translucent nature of enamel results in different enamel densities emitting different levels of fluorescence. As a result, with FEI technology, one can measure the density of tooth enamel by measuring its fluorescence when subjected to specific light wavelengths. Purpose: To determine the ability of visual examination and the instrumental procedures of the FluoreCam to monitor molar-incisor hypomineralization (MIH) lesions. Subjects and Methods: This study involved children with MIH at the Department of Paediatric Dentistry, Marmara University. In total, 11 patients with MIH were diagnosed on a visual MIH scale and evaluated with the FluoreCam. The equipment, data processing, and interaction between the equipment and operator were evaluated. Results: Fluorescent images recorded with the custom software, the clinical view, and digital numeric values were evaluated to assess the potential for use of the device in clinical practice. Conclusion: These preliminary data from an ongoing clinical study suggest that measurements with the FluoreCam are useful in monitoring MIH. This technique also provides visual and quantitative feedback to patients.

Prevalence and Pattern of Non-Syndromic Hypodontia in a Group of Turkish Children

OBJECTIVE The aim of this study was to assess the prevalence and pattern of congenital missing teeth in the permanent dentition (excluding wisdom teeth), among Turkish children in a 4-year period (2009-2012). METHODS The study group comprised 1658 children (873 girls, 785 boys). The children were examined in Department of Pediatric Dentistry, Dental School of Marmara University. A chi square test was used to determine the difference in the prevalence of hypodontia between genders. RESULTS The prevalence of hypodontia was 6.2% (6.3% girls, 6% boys) with no statistically significant difference between the genders (P=0.601). The most frequently missing tooth were the mandibular left second premolars, 63 (20.7%), followed by the mandibular right second premolars, 61(20.1%), maxillary left premolars, 31 (10.2%). There were 89 anterior and 214 posterior missing teeth. Bilateral hypodontia was observed in 70 (39 girls, 31 boys) patients (67.9%). The most common bilateral missing teeth were the mandibular second premolar (22 girls, 21 boys) (42.1%) and the maxillary second premolar (6 girls, 15 boys) (20.5%). The mandibular left second premolar (9 boys, 8 girls) was the most frequent unilaterally missing tooth (5.6%) followed by the mandibular right second premolar (10 girls, 6 boys) (5.28%). CONCLUSION The obtained results point to the importance of detailed clinical and radiographic examination. These help with long-term treatment planning according to a childs individual requirements.

Microleakage in class V cavities prepared using conventional method versus Er:YAG laser restored with glass ionomer cement or resin composite

Abstract This study evaluated the effect of tooth preparation method (diamond bur vs. Er:YAG laser) on the microleakage levels of glass ionomers and resin composite. Human permanent premolars (N = 80) were randomly divided into two groups (n = 40). Cavities on half of the teeth were prepared using diamond bur for enamel and carbide bur for dentin and the other half using Er:YAG laser. The teeth were randomly divided into four groups according to the restoration materials, namely (a) ChemFil Rock (CFR), (b) IonoluxAC (IAC), (c) EQUIA system (EQA) and one resin composite (d) AeliteLS (ALS) (n = 10 per group). Microleakage (μm) was assessed at the occlusal and gingival margins after dye penetration (0.5% basic fuchsine for 24 h). On the occlusal aspect, while the cavity preparation types significantly affected the microleakage for CFR (p = 0.015), IAC (p = 0.001) glass ionomer restorations, it did not show significant effect for glass ionomer EQA (p = 0.09) and resin composite ALS (p = 0.2). Er:YAG laser presented less microleakage compared to bur preparation in all groups except for EQA. On the gingival aspect, microleakage decreased significantly for CFR (p = 0.02), IAC (p = 0.001), except for EQA where significant increase was observed (p = 0.001) with the use of Er:YAG laser. Microleakage decrease was not significant at the gingival region between diamond bur and Er:YAG laser for ALS (p = 0.663). At the occlusal and gingival sites in all groups within each preparation method, microleakage level was not significant.