Sertac Peker

A Quantitative Analysis of a Probiotic Storage Media for Avulsed Teeth

AIM The aim of the present in vitro study was to investigate the potential of a storage medium, probiotic yogurt (Bifidibacterium animalis DN 173010) in comparison with Hanks balanced salt solution (HBSS), saline and milk in maintaining viable periodontal ligament (PDL) cells on simulated avulsed teeth. MATERIALS AND METHODS Thirty-six freshly extracted single-rooted human teeth with closed apices were divided into six experimental groups (N=6). The teeth were extracted as atraumatically as possible and washed in sterile saline solution to eliminate residual blood. Following extractions, the coronal 3 mm of PDL tissues were scraped with a #15 scalpel to remove cells that may have been damaged. The positive and negative controls corresponded to 0 minutes and an 8-hour dry time, respectively. After extraction, the positive control teeth were immediately treated with dispase and collagenase. The negative control teeth were bench-dried for 8 h, with no follow-up storage solution time, and then placed in the dispase and collagenase. The number of viable protective least significant difference PDL cells were counted under a light microscope with a hemocytometer at 20× magnification and analyzed. Statistical analysis of the data was accomplished using Nonparametric ANOVA complemented by Kruskal-Wallis Test and Dunns Multiple Comparisons Test. RESULTS Positive control was found to be significantly better than the others, there were statistically significant differences between positive control and other test groups (p=0.000). The teeth stored in positive control demonstrated the highest number of viable PDL cells followed in order by probiotic yogurt, HBSS, saline and milk. CONCLUSION Bifidibacterium animalis DN 173010 seems to be an alternative for the temporary storage of avulsed teeth, due to high number of viable PDL cells. Probiotics may be suitable transport media for avulsed teeth, but further research is warranted using the commercially available products.

Possible Medical Aetiological Factors and Characteristics of Molar Incisor Hypomineralisation in a Group of Turkish Children

Libraries have an exceptional place in the history, culture, education and scientific life of a nation. They collect all aspects of our linguistics and literacy, all out theoretical assumptions as well as all the results of experience and practice. The importance of a library is not mirrored only in the national and historical role and heritage, but in a more permanent, informational role, since a modern library must, above all, be an effective information system. Since a library of a university operates as a part of its matrix, it is easily shadowed by other forms of educational and scientific infrastructure. 50(th) anniversary of the Central Dental Library of the School of Dental Medicine University of Zagreb is an excellent opportunity to make a call to the institution and public to its unique and irreplaceable role.Sažetak Svrha:�Željele su se odrediti klinicke znacajke i moguci medicinski uzroci molarno incizivne hipominerelizacije (MIH-a) u skupini turske djece. Materijali i metode: U ispitivanju je sudjelovalo 228 djece u dobi od 7 do 14 godina. Svi su pregledani na Odjelu za djecju dentalnu medicinu Stomatoloskog fakulteta Sveucilista Marmara u Istambulu. Prenatalni i postnatalni podatci prikupljeni su popunjavanjem upitnika. Rezultati: MIH je pronađen kod 54 (24 %) djeteta, a 41 (76 %) imalo je samo ogranicena podrucja opaciteta (1. stupanj). Kod osam (15%) djevojcica i djecaka bile su obavljene atipicne restauracije (2. stupanj), a barem jedno posteruptivno ljustenje (3. stupanj) uoceno je kod njih pet (9 %). MIH je svima uglavnom zahvacao kutnjake (55 %), a ne sjekutice (45 %; p<0,001). U skupini s MIH-om bili su cesti prenatalni bronhitis i hipertenzija. Nije bilo vece razlike u medicinskoj anamnezi do dobi od tri godine između skupine s MIH-om i skupine bez njega, osim duljeg uzimanja lijekova i astme u skupini s molarno incizalnom hipomineralizacijom (p<0,05). Zakljucak:�Premda etiologija MIH-a ostaje nejasna, cest je kod djece koja neprekidno uzimaju lijekove i oboljela su od astme u prve tri godine života.

Microleakage in class V cavities prepared using conventional method versus Er:YAG laser restored with glass ionomer cement or resin composite

Abstract This study evaluated the effect of tooth preparation method (diamond bur vs. Er:YAG laser) on the microleakage levels of glass ionomers and resin composite. Human permanent premolars (N = 80) were randomly divided into two groups (n = 40). Cavities on half of the teeth were prepared using diamond bur for enamel and carbide bur for dentin and the other half using Er:YAG laser. The teeth were randomly divided into four groups according to the restoration materials, namely (a) ChemFil Rock (CFR), (b) IonoluxAC (IAC), (c) EQUIA system (EQA) and one resin composite (d) AeliteLS (ALS) (n = 10 per group). Microleakage (μm) was assessed at the occlusal and gingival margins after dye penetration (0.5% basic fuchsine for 24 h). On the occlusal aspect, while the cavity preparation types significantly affected the microleakage for CFR (p = 0.015), IAC (p = 0.001) glass ionomer restorations, it did not show significant effect for glass ionomer EQA (p = 0.09) and resin composite ALS (p = 0.2). Er:YAG laser presented less microleakage compared to bur preparation in all groups except for EQA. On the gingival aspect, microleakage decreased significantly for CFR (p = 0.02), IAC (p = 0.001), except for EQA where significant increase was observed (p = 0.001) with the use of Er:YAG laser. Microleakage decrease was not significant at the gingival region between diamond bur and Er:YAG laser for ALS (p = 0.663). At the occlusal and gingival sites in all groups within each preparation method, microleakage level was not significant.