Bidyut K. Datta

The Analgesic and Anti-Inflammatory Activities of the Extracts of Phyllanthus reticulatus. in Mice Model

Abstract The petroleum ether, ethyl acetate, and methanol extracts of Phyllanthus reticulatus. Poir. (Euphorbiaceae) were chosen for pharmacological screening. In the acetic acid-induced writhing test, the ethyl acetate extract in doses of 150 and 300 mg/kg showed 51.23 and 65.12% inhibition of writhing, respectively. A significant elongation of tail-flick time was evident both in the ethyl acetate and the methanol extracts (42.38 and 60.49%) only at the 300 mg/kg dose level. In carrageenan-induced rat paw edema model, the methanol extract at the 300 mg/kg dose level showed 40.03% inhibition of edema at the end of 4 h. These results demonstrated that the extracts of P. reticulatus. possess significant analgesic and anti-inflammatory properties.

Analgesic and diuretic properties of α-santalone from Polygonum flaccidum.

Polygonum flaccidum Meissn. is an annual herb, native to Bangladesh, and well known for its analgesic, anti‐inflammatory, diuretic, purgative and insecticidal properties, and also for its use against snake‐bites. The analgesic and the diuretic properties of α‐santalone (1), isolated from the aerial parts of Polygonum flaccidum, were assessed by the acetic‐acid‐induced writhing method and the Lipschitz test, respectively. Complete 1H and 13C NMR data of 1 are also presented. Copyright

Quercetin 3-O-(6"-galloyl)-beta-D-galactoside from Polygonum viscosum (Polygonaceae).

1. Subject and source Polygonum viscosum Buch.-Ham. Ex D. Don (Family: Polygonaceae), commonly known as`Bishkatalia, is an erect annual herb indigenous to Nepal and widely distributed in Bangladesh, northeast India, China and Japan. The plants were collected from Panchari, Chittagong and a voucher specimen (voucher no 764) developed in the Herbarium of the To our knowledge no report on the phytochemical or pharmacological studies on P. viscosum is available to date.

Protective effects of Flacourtia indica aerial parts extracts against paracetamol-induced hepatotoxiciy in rats

Abstract The petroleum ether, ethyl acetate and methanol extracts of the aerial parts of Flacourtia indica (Burm. f.) Merr., were evaluated for hepato-protective properties. In paracetamol-induced hepatic necrosis in rat models, all extracts were found to reduce serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and serum alkaline phosphatase (SAP). The most significant reduction of the serum level of SGOT and SGPT were exhibited by petroleum ether and ethyl acetate extracts at a single oral of dose of 1.5 g/kg of body weight with a reduction of 29.0% SGOT & 24.0% SGPT level by petroleum ether extract, and 10.57% SGOT & 6.7% SGPT level by ethyl acetate extract compared to paracetamol (3 g/kg of body weight) treated animals. Histopathological examination also showed good recovery of paracetamol-induced necrosis by petroleum ether and ethyl acetate extracts. On the other hand, the methanol extract obtained by successive cold extraction did not show any remarkable effect on paracetamol-induced hepatic necrosis. The hepatoprotective effects exhibited by petroleum ether and ethyl acetate extract might be mediated through the inhibition of microsomal drug metabolizing enzymes.

Anti-cholinergic, Cytotoxic and Anti-HIV-1 Activities of Sesquiterpenes and a Flavonoid Glycoside from the Aerial Parts of Polygonum viscosum

The anti-cholinergic, cytotoxic and anti-HIV-1 activities of four sesquiterpenes, viscosumic acid, viscozulenic acid, viscoazucine and viscoazulone, and a flavonoid glycoside, quercetin 3-O-(6″-feruloyl)-β-d-galactopyranoside, isolated from the aerial parts of Polygonum viscosum have been assessed. Among these compounds, quercetin 3-O-(6″-feruloyl)-β-d-galactopyranoside exhibited significant cytotoxic activity (IC50 = 13.33 µg/mL) against the ovarian cancer cell line (OVCAR-3), anti-HIV-1 activity (IC50 = 25.61 µg/mL) and mild anti-cholinergic activity. Only one sesquiterpene, viscoazulone, showed anti-HIV-1 activity (IC50 = 33.13 µg/mL).

Viscozulenic acid : a novel sesquiterpene acid from Polygonum viscosum

High-performance liquid chromatographic analysis of a methanol extract of Polygonum viscosum has yielded a novel sesquiterpene acid, 6-hydroxy-4-methoxycarbonyl-7-(1-methylethyl)-3,3a,6,7,8,8a-hexahydroazulene-1-carboxylic acid (named viscozulenic acid) [1], the structure of which was unambiguously elucidated by UV, HR-EIMS, and a series of 1D and 2D NMR experiments.

Flavone and α-santalene derivatives from Polygonum flaccidum

Abstract Chemical investigation of Polygonum flaccidum revealed the presence of a new acylflavone and an α-santalene derivative. The structures of the compounds were elucidated by high field 1H NMR spectroscopic techniques.

Antinociceptive, anti-inflammatory and diuretic properties of Polygonum barbatum (L.) Hara var. barbata

The antinociceptive, anti-inflammatory and diuretic properties of the extracts of P. barbatum (L.) Hara var. barbata, Polygonaceae, at the doses of 200 and 400 mg/kg body weight, were evaluated in mice/rat models using, respectively, the acetic-acid-induced writhing method, the carrageenan-induced edema test and the Lipschitz method. In the acetic-acid-induced writhing test in mice, all extracts displayed a dose dependent analgesic effect. The most potent analgesic activity was observed with the petroleum ether extract at the dose of 400 mg/kg body weight with an inhibition of writhing response 46.8% compared to 62.2% for the positive control aminopyrine. Petroleum ether extract at the dose of 400 mg/kg body weight also displayed the highest levels of anti-inflammatory activity after 2 h with the 39.3% inhibition of paw edema, and this effect was better than the effect observed by the conventional anti-inflammatory agent phenylbutazone (maximum inhibition of 38.3% after 4 h). All extracts increased urine volume in a dose-dependent manner, and the ethyl acetate extract showed a significant level of diuresis comparable to that of the standard diuretic agent furosemide.

Quercetin 3-O-(6′′-caffeoyl)-β-d-galactopyranoside from Polygonum viscosum

Abstract The methanol extract of the whole plant parts of Polygonum viscosum has yielded a flavonol glycoside, 3-O-(6′′-caffeoyl)-β- d -galactopyranoside (1), the structure of which has been determined unambiguously by UV and a series of one- and two-dimensional NMR experiments, notably, 1H, 13C, DEPT, COSY45, HMBC and HMQC.

Further Sesquiterpenes from Polygonum Viscosum (Polygonaceae)

Two new sesquiterpenes, 4-methoxycarbonyl-7-(1-methylethyl)-6-oxo-3,3a,7,8,8a-pentahydroazulene-1-carboxylic acid (viscoazusone) and 1,4-dimethoxy-carbonyl-7-(1-methylethyl)-6-oxo-3,3a,7,8,8a-pentahydroazulene (viscoazulone), were isolated from the whole plant of Polygonum viscosum . The structures of these compounds were determined by spectroscopic means.