Binit Shrestha

In vitro effects of Salvia officinalis L. essential oil on Candida albicans.

OBJECTIVE To determine the anticandidal activities of Salvia officinalis L. (S. officinalis) essential oil against Candida albicans (C. albicans) and the inhibitory effects on the adhesion of C. albicans to polymethyl methacrylate (PMMA) resin surface. METHODS Disc diffusion method was first used to test the anticandidal activities of the S. officinalis L. essential oil against the reference strain (ATCC 90028) and 2 clinical strains of C. albicans. Then the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) were determined by modified membrane method. The adhesion of C. albicans to PMMA resin surface was assessed after immersion with S. officinalis L. essential oil at various concentrations of 1×MIC, 0.5×MIC and 0.25×MIC at room temperature for 30 min. One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukeys test was used for multiple comparisons. RESULTS S. officinalis L. essential oil exhibited anticandidal activity against all strains of C. albicans with inhibition zone ranging from 40.5 mm to 19.5 mm. The MIC and MLC of the oil were determined as 2.780 g/L against all test strains. According to the effects on C. albicans adhesion to PMMA resin surface, it was found that immersion in the essential oil at concentrations of 1×MIC (2.780 g/L), 0.5×MIC (1.390 g/L) and 0.25×MIC (0.695 g/L) for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner (P<0.05). CONCLUSIONS S. officinalis L. essential oil exhibited anticandidal activities against C. albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface. With further testing and development, S. officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

In vitro antimicrobial effects of grape seed extract on peri-implantitis microflora in craniofacial implants

OBJECTIVE To determine the antimicrobial effects of grape seed on peri-implantitis microflora. METHODS The grape seed extract was tested against peri-implantitis microflora most commonly found in craniofacial implants including reference strains of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Candida albicans (C. albicans) and clinical strains of S. aureus, Klebsiella pneumonia (K. pneumonia) and Candida parapsilosis (C. parapsilosis) by disk diffusion test. Minimum inhibitory concentrations (MIC) and minimum cidal concentrations (MCC) were determined using modified agar dilution millpore method. The extract was further combined with polyethylene glycol and propylene glycol, and was tested for antimicrobial effects. RESULTS Grape seed extract showed positive inhibitory effects with S. aureus at MIC of 0.625 mg/mL and MCC of 1.25 mg/mL respectively. However the extracts showed minimal or no reactivity against strains of E. coli, K. pneumonia, C. parapsilosis and C. albicans. The use of grape seed extract in combination with polyethylene glycol and propylene glycol also showed dose dependent inhibitory effect on S. aureus. CONCLUSIONS The results of the study showed that grape seed has potential antimicrobial effects which can be further studied and developed to be used in the treatment of infected skin-abutment interface of craniofacial implants.

Late effects of orbital enucleation and radiation on maxillofacial prosthetic rehabilitation: A clinical report.

The retinoblastoma is one of the most common tumors of the eye diagnosed in childhood. The treatment for patients with retinoblastoma includes surgical removal of the lesion along with adjunctive chemotherapy and radiotherapy. If the tumor is controlled, these treatments can have secondary adverse effects pertaining to the growth and development of orofacial structures in young patients. This clinical report describes the prosthetic rehabilitation of a patient who underwent enucleation followed by radiation therapy and adjunctive chemotherapy to treat the primary diagnosis of retinoblastoma. This therapy resulted in a combination of dental and facial growth and developmental abnormalities.

Silicone nasal prosthesis retained by an intranasal stent: a clinical report.

Nasal defects after tumor excision can leave a patient functionally and esthetically impaired. Loss of nasal septal cartilage support causes the soft tissue to collapse or undergo stenosis, further compounding the problem. Intranasal stents can be used to maintain the patency of such nasal defects. This clinical report describes the use of an acrylic resin nasal stent bonded to a silicone nasal prosthesis to rehabilitate a patient with a nasal defect.

Orbital prosthesis fabrication: current challenges and future aspects

Orbital defect, arising from tumor-related resections, trauma, and congenital anoma- lies, can lead to negative functional and psychological effects in the patients. Rehabilitation with an orbital prosthesis can provide satisfactory aesthetic outlook and can be combined with surgical reconstruction to improve the retention. Despite less than ideal properties, silicone elastomer is the most commonly used material to fabricate orbital prosthesis. Several studies have been conducted on developing newer materials and improving the properties of silicone elastomer. Craniofacial implants offer greater retention compared to adhesives, but they have been observed to have a higher risk of implant failure and related side effects when placed in irradiated bone. This review gives a scope on understanding the current challenges faced during fabrication of orbital prosthesis and the future directions of this field.

Pain and quality of life related to suture removal after 3 or 7 days at the extraction sites of impacted lower third molars

Background This study aimed to evaluate the patients pain and quality of life after suture removal at either 3 or 7 days following the bilateral surgical extraction of impacted lower third molars. Methods This study was a prospective, randomized controlled clinical trial carried out in 30 patients, who acted as their own control. Each patient required the bilaterally impacted mandibular third molars to be extracted. The impacted teeth were removed and the wound margins were approximated and sutured with black braided silk. The suture material was removed on day 3 on one side and on day 7 on the other. Each participant was asked to complete a questionnaire after the removal of the suture material on each designated day. Results Regarding overall clinical symptoms, the mean VAS scores of male and female participants on day 3 were not significantly different from those on day 7. A significant difference was found in female participants, in that overall daily activity was better on day 7. There were significant differences in the ability to smile and laugh in both sexes and the ability to chew in the male participants was better on day 7. Conclusions There were no significant differences in the patients pain and quality of life between suture removal on day 3 or on day 7 following surgery to remove impacted lower third molars.

Prosthodontic rehabilitation of congenital auricular defect: a clinical report.

This clinical report presents the prosthetic rehabilitation of a congenital auricular defect. The rehabilitation was initially performed with an adhesive retained silicone prosthesis. After careful evaluation of the patients retentive needs, two endo-osseous craniofacial implants were placed in the mastoid region. After integration, an implant-retained silicone prosthesis was fabricated using a Hader bar with two ERA attachments, which restored the defect with good aesthetic and functional outcome. Prosthetic rehabilitation is a viable treatment option for restoring auricular defects and can be indicated in situations where surgical reconstruction may not be feasible.

Rapid fabrication of silicone orbital prosthesis using conventional methods

Restoration of orbital defects with silicone prosthesis has been a well-documented and accepted treatment option. Adhesive retained prosthesis offer the patients with adequate retention and treatment satisfaction. However, marginal breakdown and discoloration are common problems associated with these prostheses, necessitating their refabrication. Fabrication of a silicone orbital prosthesis is time consuming and requires multiple clinical and laboratory procedures. This technical article describes simple and cost effective steps for rapid fabrication of a silicone prosthesis using conventional methods.

A two-step functional impression technique for the fabrication of an implant-retained silicone auricular prosthesis

Excessive movement of the underlying tissue bed can lead to poor retention of an implant-retained silicone auricular prosthesis. This article describes a 2-step impression technique with a secondary functional pickup impression of the wax pattern. A wide range of tissue movements can be recorded while maintaining a stable relationship between the abutment analog assembly and retentive elements. The definitive cast is modified accordingly to provide an accurate reproduction of the patients tissues during function, thus, increasing the overall retention and stability of the definitive prosthesis.

Rapid fabrication of a digital prosthesis.

Finger prosthesis often needs refabrication due to its discoloration following use. This article presents a novel, economical, and cost-effective technique to duplicate the patient׳s existing prosthesis to obtain a new wax replica, which is then clinically tried and processed to obtain new silicone finger prosthesis. This technique requires comparatively less clinical and laboratory steps as to fabricate an entirely new prosthesis. The newly fabricated silicone finger prosthesis has the fit and marginal adaptation of the patient׳s existing prosthesis but the esthetics is improved.