C. Arden Miller

Comparison of Methods for recovering Poliomyelitis Viruses from Human Sources.

Summary One hundred and four fecal and oropharyngeal specimens obtained directly from human sources were studied in 3 types of tissue cultures. The virus recovery rates among fecal samples were of the same order regardless of the type of tissue culture used. Under the conditions of the study, monkey kidney epithelial cell cultures were superior to HeLa or testicular cultures in the primary isolation of poliomyelitis virus from oropharyngeal specimens, A comparison of tests with fecal samples in monkeys and in tissue culture indicated that the latter was as good as, if not better than, monkeys in detecting poliomyelitis virus.

Growth of Poliomyelitis Viruses in Large Stationary Cultures.

Summary Poliomyelitis viruses were propagated in large stationary tissue cultures of plasma imbedded monkey testes. Large volumes of supernatant fluids with high virus titers were achieved. The virus yield, as measured by titer and duration of maximum growth, was almost identical with that obtained in roller tubes, and greater than that reported for suspended cell flask cultures. The method had another advantage in that equipment for rotating bottles was not required. Since the Roux flasks could be stacked one on top of another, a minimum of incubator space was required.

Problems associated with routine immunizations.

(1957). Problems Associated With Routine Immunizations. Postgraduate Medicine: Vol. 22, No. 5, pp. 445-454.