Canh Le Tien

Mechanical and barrier properties of nanocrystalline cellulose reinforced chitosan based nanocomposite films

Nanocrystalline cellulose (NCC) reinforced chitosan-based biodegradable films were prepared by solution casting. The NCC content in the films was varied from 1 to 10% (dry wt. basis). It was found that the tensile strength (TS) of the nanocomposite films with 5% (w/w) NCC content was optimum with an improvement of 26% compared to the control chitosan films. Incorporation of NCC also significantly improved barrier properties. Water vapor permeability (WVP) of the chitosan/NCC films was decreased by 27% for the optimum 5% (w/w) NCC content. Swelling studies revealed a decrease in water uptake of the NCC-reinforced chitosan films. Analyses of thermal properties showed no significant effect of NCC whereas X-ray diffraction studies confirmed the appearance of crystalline peaks in the nanocomposite films. Surface morphology of the films was investigated by scanning electron microscopy and it was found that NCC was dispersed homogenously into chitosan matrix.

N-acylated chitosan: hydrophobic matrices for controlled drug release.

N-acylation of chitosan with various fatty acid (C(6)-C(16)) chlorides increased its hydrophobic character and made important changes in its structural features. Unmodified chitosan exhibited a low degree of order (DO) and a weak tablet crushing strength. Chitosan acylated with a short chain length (C(6)) possessed similar properties, but exhibited significant swelling. Acylation with longer side chains (C(8)-C(16)) resulted in a higher DO and crushing strength but lower swelling. The best mechanical characteristics and drug release properties were found for palmitoyl chitosan (substitution degree 40-50%) tablets with 20% acetaminophen as a tracer. The high stability of these monolithic tablets appears to be due to hydrophobic interactions between side chains, as shown by a more organized structure. Infrared spectroscopy, X-ray diffractometry and proton nuclear magnetic resonance analyses of palmitoyl chitosan were consistent with a hydrophobic self-assembling model. Drug dissolution kinetics showed longer release times for higher degrees of functionalization, i.e. 30 h (for 47% substitution) and 90 h (for 69% substitution), suggesting palmitoyl chitosan excipients as interesting candidates for oral and subdermal pharmaceutical applications.

Nanocrystalline cellulose (NCC) reinforced alginate based biodegradable nanocomposite film.

Nanocrystalline cellulose (NCC) reinforced alginate-based nanocomposite film was prepared by solution casting. The NCC content in the matrix was varied from 1 to 8% ((w/w) % dry matrix). It was found that the nanocomposite reinforced with 5 wt% NCC content exhibits the highest tensile strength which was increased by 37% compared to the control. Incorporation of NCC also significantly improved water vapor permeability (WVP) of the nanocomposite showing a 31% decrease due to 5 wt% NCC loading. Molecular interactions between alginate and NCC were supported by Fourier Transform Infrared Spectroscopy. The X-ray diffraction studies also confirmed the appearance of crystalline peaks due to the presence of NCC inside the films. Thermal stability of alginate-based nanocomposite films was improved after incorporation of NCC.

Improvement of water barrier property of paperboard by coating application with biodegradable polymers.

Biopolymeric coatings were prepared and applied onto paperboard to improve its water barrier property. To prepare whey protein isolate (WPI)/cellulose-based films, WPI and glycerol were dissolved in water with glutaraldehyde (cross-linking agent) and cellulose xanthate. The solution was cast, dried, and insolubilized by entrapment of WPI in regenerated cellulose. Films were combined with beeswax (BW) into a bilayer coating system and then applied onto paperboard by heating compression. Another coating solution consisting of poly(vinyl butyral) (PVB)/zein was prepared by dissolving poly(vinyl alcohol) (PVA) and zein in 70% ethanol with glutaraldehyde and butyraldehyde (functionalization agent). The PVB/zein solution was applied onto paperboard after BW was sprayed. The structure of the PVB/zein-based coatings was analyzed by Fourier transform infrared spectroscopy (FTIR). The water vapor barrier property of coated paperboards was evaluated by water vapor transmission rate (WVTR) measurements. From the FTIR spectra, PVA functionalization after cross-linking and efficient acetalization into PVB were confirmed. WPI/cellulose and PVB/zein coating treatments improved the water barrier properties of paperboard by decreasing the WVTR by 77-78%. Although the BW coating was more efficient (decrease of WVTR by 89%), bilayer coatings composed of BW and polymer coatings had a stronger barrier effect with a decrease of WVTR to 92-95%, hence approaching commercial attributes required to ensure water vapor barrier in paperboard-based food containers (10 g/m(2).day). These results suggest that surface coating by biodegradable polymers may be utilized for the manufacture of paperboard containers in industrial applications.

20 – Edible films and coatings from nonstarch polysaccharides

Publisher Summary This chapter focuses on the polysaccharide-based films that have the potential to be used in a variety of applications. These films can be used to extend the shelf life of fruits, vegetables, seafood, meats, and confectionery products by preventing dehydration, oxidation rancidity, surface browning, and oil diffusion, as well as by their ability to modify the internal atmosphere in fruit and vegetable packaging. Moreover, when applied to fruits and vegetables, polysaccharide-based coatings can improve the physicochemical, nutritional, and sensorial properties of the products. Several studies have demonstrated that the incorporation of active compounds like antimicrobials, antioxidants, or bacteriocins in edible films and coatings can improve the microbial safety and the shelf life, and can be used to stabilize the quality of the products during storage. The development of new technologies (functionalization, cross-linking) to improve the film properties (control release, bioactivity protection, resistance to water) of active packaging and coatings is a major focus for future research.

The effect of ethylene oxide sterilization on the surface chemistry and in vitro cytotoxicity of several kinds of chitosan

The surfaces of three chitosan samples, differing only in their degrees of deacetylation and of carboxyethyl chitosan were chemically characterized by X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectroscopy, X-ray diffraction, and Fourier transform infrared, both before and after sterilization with ethylene oxide. Unexpected elemental ratios suggest that surface chemical modification occurred during the processing of the original chitin, with further surface modification on subsequent sterilization, despite previous reports to the contrary. Cell viability was evaluated by direct contact methyl thiazole tetrazolium and lactate dehydrogenase assays between the chitosan particles and A549 human epithelial cells, which demonstrated that the modifications incurred on sterilization are reflected in biocompatibility changes. All the samples were found to be biocompatible and nontoxic before sterilization and remained so subsequently.