Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Caren C. Helbing is active.

Publication


Featured researches published by Caren C. Helbing.


Environmental Toxicology and Chemistry | 2004

Toxicity of glyphosate‐based pesticides to four North American frog species

Christina M. Howe; Michael Berrill; Bruce D. Pauli; Caren C. Helbing; Kate Werry; Nik Veldhoen

Glyphosate-based herbicides are among the most widely used pesticides in the world. We compared the acute toxicity of the glyphosate end-use formulation Roundup Original to four North American amphibian species (Rana clamitans, R. pipiens, R. sylvatica, and Bufo americanus) and the toxicity of glyphosate technical, the polyethoxylated tallowamine surfactant (POEA) commonly used in glyphosate-based herbicides, and five newer glyphosate formulations to R. clamitans. For R. clamitans, acute toxicity values in order of decreasing toxicity were POEA > Roundup Original > Roundup Transorb > Glyfos AU; no significant acute toxicity was observed with glyphosate technical material or the glyphosate formulations Roundup Biactive, Touchdown, or Glyfos BIO. Comparisons between the four amphibian species showed that the toxicity of Roundup Original varied with species and developmental stage. Rana pipiens tadpoles chronically exposed to environmentally relevant concentrations of POEA or glyphosate formulations containing POEA showed decreased snout-vent length at metamorphosis and increased time to metamorphosis, tail damage, and gonadal abnormalities. These effects may be caused, in some part, by disruption of hormone signaling, because thyroid hormone receptor beta mRNA transcript levels were elevated by exposure to formulations containing glyphosate and POEA. Taken together, the data suggest that surfactant composition must be considered in the evaluation of toxicity of glyphosate-based herbicides.


Environmental Health Perspectives | 2009

Integrating omic technologies into aquatic ecological risk assessment and environmental monitoring: hurdles, achievements, and future outlook.

Graham van Aggelen; Gerald T. Ankley; William S. Baldwin; Daniel W. Bearden; William H. Benson; J. Kevin Chipman; Tim Collette; John A. Craft; Nancy D. Denslow; Michael R. Embry; Francesco Falciani; Stephen G. George; Caren C. Helbing; Paul F. Hoekstra; Taisen Iguchi; Yoshi Kagami; Ioanna Katsiadaki; Peter Kille; Li Liu; Peter G. Lord; Terry McIntyre; Anne O'Neill; Heather Osachoff; Ed J. Perkins; Eduarda M. Santos; Rachel C. Skirrow; Jason R. Snape; Charles R. Tyler; Don Versteeg; Mark R. Viant

Background In this commentary we present the findings from an international consortium on fish toxicogenomics sponsored by the U.K. Natural Environment Research Council (Fish Toxicogenomics—Moving into Regulation and Monitoring, held 21–23 April 2008 at the Pacific Environmental Science Centre, Vancouver, BC, Canada). Objectives The consortium from government agencies, academia, and industry addressed three topics: progress in ecotoxicogenomics, regulatory perspectives on roadblocks for practical implementation of toxicogenomics into risk assessment, and dealing with variability in data sets. Discussion Participants noted that examples of successful application of omic technologies have been identified, but critical studies are needed to relate molecular changes to ecological adverse outcome. Participants made recommendations for the management of technical and biological variation. They also stressed the need for enhanced interdisciplinary training and communication as well as considerable investment into the generation and curation of appropriate reference omic data. Conclusions The participants concluded that, although there are hurdles to pass on the road to regulatory acceptance, omics technologies are already useful for elucidating modes of action of toxicants and can contribute to the risk assessment process as part of a weight-of-evidence approach.


Environmental Health Perspectives | 2006

PCB-related alteration of thyroid hormones and thyroid hormone receptor gene expression in free-ranging harbor seals (Phoca vitulina)

Maki Tabuchi; Nik Veldhoen; Neil Dangerfield; Steven J. Jeffries; Caren C. Helbing; Peter S. Ross

Persistent organic pollutants are environmental contaminants that, because of their lipophilic properties and long half-lives, bioaccumulate within aquatic food webs and often reach high concentrations in marine mammals, such as harbor seals (Phoca vitulina). Exposure to these contaminants has been associated with developmental abnormalities, immunotoxicity, and reproductive impairment in marine mammals and other high-trophic-level wildlife, mediated via a disruption of endocrine processes. The highly conserved thyroid hormones (THs) represent one vulnerable endocrine end point that is critical for metabolism, growth, and development in vertebrates. We characterized the relationship between contaminants and specific TH receptor (TR ) gene expression in skin/blubber biopsy samples, as well as serum THs, from free-ranging harbor seal pups (n = 39) in British Columbia, Canada, and Washington State, USA. We observed a contaminant-related increase in blubber TR-α gene expression [total polychlorinated biphenyls (∑PCBs); r = 0.679; p < 0.001] and a concomitant decrease in circulating total thyroxine concentrations (∑PCBs; r = −0.711; p < 0.001). Consistent with results observed in carefully controlled laboratory and captive feeding studies, our findings suggest that the TH system in harbor seals is highly sensitive to disruption by environmental contaminants. Such a disruption not only may lead to adverse effects on growth and development but also could have important ramifications for lipid metabolism and energetics in marine mammals.


Environmental Science & Technology | 2011

Effects of Triclocarban, Triclosan, and Methyl Triclosan on Thyroid Hormone Action and Stress in Frog and Mammalian Culture Systems

Ashley Hinther; Caleb M. Bromba; Jeremy E. Wulff; Caren C. Helbing

Triclosan (TCS) and triclocarban (TCC) are widely used broad spectrum bactericides that are common pollutants of waterways and soils. Methyl triclosan (mTCS) is the predominant bacterial TCS metabolite. Previous studies have shown that TCS disrupts thyroid hormone (TH) action; however, the effects of mTCS or TCC are not known. The present study uses the cultured frog tadpole tail fin biopsy (C-fin) assay and the TH-responsive rat pituitary GH3 cell line to assess the effects of these three chemicals (1-1000 nM) on TH signaling and cellular stress within 48 h. mRNA abundance of TH receptor β, Rana larval keratin type I (TH-response), heat shock protein 30, and catalase (stress-response) was measured using quantitative real-time polymerase chain reaction in the C-fin assay. The TH-responsive gene transcripts encoding growth hormone, deiodinase I, and prolactin were measured in GH3 cells with the heat shock protein 70 transcript acting as a cellular stress indicator. We found alteration of stress indicators at a wide range of concentrations of TCS, mTCS, and TCC in both test systems. mTCS and TCC affected TH-responsive gene transcripts at the highest concentration in mammalian cells, whereas a modest effect included lower concentrations in the C-fin assay. In contrast, TCS did not affect TH-responsive transcripts. These results identify nontarget biological effects of these bacteriocides on amphibian and mammalian cells and suggest the TH-disrupting effects observed for TCS could be mediated through its metabolite.


BMC Biology | 2009

Characterization of the histone H2A.Z-1 and H2A.Z-2 isoforms in vertebrates.

Deanna Dryhurst; Toyotaka Ishibashi; Kristie L. Rose; José M. Eirín-López; Darin McDonald; Begonia Silva-Moreno; Nik Veldhoen; Caren C. Helbing; Michael J. Hendzel; Jeffrey Shabanowitz; Donald F. Hunt; Juan Ausió

BackgroundWithin chromatin, the histone variant H2A.Z plays a role in many diverse nuclear processes including transcription, preventing the spread of heterochromatin and epigenetic transcriptional memory. The molecular mechanisms of how H2A.Z mediates its effects are not entirely understood. However, it is now known that H2A.Z has two protein isoforms in vertebrates, H2A.Z-1 and H2A.Z-2, which are encoded by separate genes and differ by 3 amino acid residues.ResultsWe report that H2A.Z-1 and H2A.Z-2 are expressed across a wide range of human tissues, they are both acetylated at lysine residues within the N-terminal region and they exhibit similar, but nonidentical, distributions within chromatin. Our results suggest that H2A.Z-2 preferentially associates with H3 trimethylated at lysine 4 compared to H2A.Z-1. The phylogenetic analysis of the promoter regions of H2A.Z-1 and H2A.Z-2 indicate that they have evolved separately during vertebrate evolution.ConclusionsOur biochemical, gene expression, and phylogenetic data suggest that the H2A.Z-1 and H2A.Z-2 variants function similarly yet they may have acquired a degree of functional independence.


Developmental Dynamics | 2002

Distinctive gene profiles occur at key points during natural metamorphosis in the Xenopus laevis tadpole tail.

Nik Veldhoen; Doug Crump; Kate Werry; Caren C. Helbing

Thyroid hormones (THs) are essential for tadpole metamorphosis into a juvenile frog; however, a complex interplay between additional hormones and signaling events also contributes to this dramatic developmental phase. A major mechanism of TH action is the nuclear receptor‐mediated regulation of gene transcription of responsive genes. By using the precocious metamorphic model, several genes have been identified as TH responsive in the regressing tail. Many of these genes also exhibit altered expression during natural metamorphosis. Although identification of these genes provides insight into the mechanism whereby TH acts, complex interplay between TH and other hormones and the developmental stage‐dependency of tissue responses contribute to the timing and coordination of metamorphic events. We investigated the temporal gene expression profile in Xenopus laevis tadpole tails from premetamorphosis through metamorphic climax by using a combination of a novel frog cDNA array containing 420 genes and quantitative real‐time PCR. Seventy‐nine genes were identified whose steady‐state mRNA expression levels were altered in the tadpole tail during natural metamorphosis, of which 34 have previously been identified to be TH responsive in frogs or mammals. Of these genes, 75 clustered into 13 groups that displayed distinct developmental expression profiles. The levels of 28 transcripts were altered during premetamorphosis, 31 during prometamorphosis, and 43 with the onset of tail regression. This work establishes an important baseline for determining the mechanisms whereby tissues undergo differing metamorphic fates.


Environmental Science & Technology | 2011

PCB-associated changes in mRNA expression in killer whales (Orcinus orca) from the NE Pacific Ocean.

Andrea H. Buckman; Nik Veldhoen; Graeme M. Ellis; John K. B. Ford; Caren C. Helbing; Peter S. Ross

Killer whales in the NE Pacific Ocean are among the worlds most PCB-contaminated marine mammals, raising concerns about implications for their health. Sixteen health-related killer whale mRNA transcripts were analyzed in blubber biopsies collected from 35 free-ranging killer whales in British Columbia using real-time quantitative polymerase chain reaction. We observed PCB-related increases in the expression of five gene targets, including the aryl hydrocarbon receptor (AhR; r(2) = 0.83; p < 0.001), thyroid hormone α receptor (TRα; r(2) = 0.64; p < 0.001), estrogen α receptor (ERα; r(2) = 0.70; p < 0.001), interleukin 10 (IL-10; r(2) = 0.74 and 0.68, males and females, respectively; p < 0.001), and metallothionein 1 (MT1; r(2) = 0.58; p < 0.001). Best-fit models indicated that population (dietary preference), age, and sex were not confounding factors, except for IL-10, where males differed from females. While the population-level consequences are unclear, the PCB-associated alterations in mRNA abundance of such pivotal end points provide compelling evidence of adverse physiological effects of persistent environmental contaminants in these endangered killer whales.


Ecotoxicology and Environmental Safety | 2012

Molecular profiling of marine fauna: integration of omics with environmental assessment of the world's oceans.

Nik Veldhoen; Michael G. Ikonomou; Caren C. Helbing

Many species that contribute to the commercial and ecological richness of our marine ecosystems are harbingers of environmental change. The ability of organisms to rapidly detect and respond to changes in the surrounding environment represents the foundation for application of molecular profiling technologies towards marine sentinel species in an attempt to identify signature profiles that may reside within the transcriptome, proteome, or metabolome and that are indicative of a particular environmental exposure event. The current review highlights recent examples of the biological information obtained for marine sentinel teleosts, mammals, and invertebrates. While in its infancy, such basal information can provide a systems biology framework in the detection and evaluation of environmental chemical contaminant effects on marine fauna. Repeated evaluation across different seasons and local marine environs will lead to discrimination between signature profiles representing normal variation within the complex milieu of environmental factors that trigger biological response in a given sentinel species and permit a greater understanding of normal versus anthropogenic-associated modulation of biological pathways, which prove detrimental to marine fauna. It is anticipated that incorporation of contaminant-specific molecular signatures into current risk assessment paradigms will lead to enhanced wildlife management strategies that minimize the impacts of our industrialized society on marine ecosystems.


Visual Neuroscience | 2006

Spatio-temporal characterization of retinal opsin gene expression during thyroid hormone-induced and natural development of rainbow trout

Kathy Veldhoen; W. Ted Allison; Nik Veldhoen; Bradley R. Anholt; Caren C. Helbing; Craig W. Hawryshyn

The abundance and spatial distribution of retinal cone photoreceptors change during thyroid hormone (TH)-induced and natural development of rainbow trout (Oncorhynchus mykiss). These changes are thought to allow the fish to adapt to different photic environments throughout its life history. To date, the ontogeny of rainbow trout cone photoreceptors has been examined using physiological and morphological approaches. In this study, we extended these observations by measuring opsin gene expression in retinal quadrants during natural and TH-induced development. Gene expression during natural development was investigated in retinae from fish at both parr and smolt stages. The role of TH in modulating opsin gene expression was determined in TH-treated parr and control fish sampled after two, nine, and 22 days of treatment. Total RNA was isolated from each retinal quadrant and steady-state opsin mRNA levels were measured using reverse transcriptase real-time quantitative polymerase chain reaction (QPCR) analysis. Expression of ultraviolet-sensitive opsin (SWS1), rod opsin (RH1), middle wavelength-sensitive opsin (RH2), and long wavelength-sensitive opsin (LWS) transcripts vary spatially in the parr retina. Smolts, compared to parr, had downregulated SWS1 expression in all quadrants, lower LWS expression dorsally, higher RH1 expression nasally, and higher RH2 expression dorsally. In TH-treated parr, SWS1 opsin expression was downregulated in the nasal quadrants by two days. SWS1 displayed the greatest degree of downregulation in all quadrants after nine days of treatment, with an increase in short wavelength-sensitive (SWS2) and RH2 opsin mRNA expression in the temporal quadrants. This study reveals that opsin genes display spatially significant differences within rainbow trout retina in their level of mRNA expression, and that regulation of opsin expression is a dynamic process that is influenced by TH. This is particularly evident for SWS1 gene expression in parr following TH-induced and natural development.


Toxicological Sciences | 2011

Triclosan Affects Thyroid Hormone–Dependent Metamorphosis in Anurans

Caren C. Helbing; Graham van Aggelen; Nik Veldhoen

Our original work on Rana catesbeiana (bullfrog) tadpoles exposed to triclosan (TCS) during induction of precocious metamorphosis that was presented in Veldhoen et al. (2006) suggested that frog tadpoles exposed to the antibacterial agent exhibit changes through prometamorphosis. In a recent study, Fort et al. (2010) exposed premetamorphic Xenopus laevis tadpoles to triclosan for 21 days into prometamorphosis. This work, published in Toxological Sciences (Fort et al., 2010), stated that environmentally relevant TCS concentrations do not alter the normal course of thyroid-mediated metamorphosis in this standard anuran model. Herein, we describe the apparent discrepancy between these two studies, and on examination of the data presented in the study of Fort et al. (2010), we do not come to the same conclusion that triclosan had no effects on metamorphosis. Fort et al. (2010) stated that ‘‘larval developmental stage at exposure day 21 was not significantly different from controls based on observed parameters.’’ A major indicator of exposure effect is progression through the developmental stages as defined by Nieuwkoop and Faber (NF; Nieuwkoop and Faber, 1994). The relevant stages in the study are between NF stages 54 and 66; the range of stages attained by NF stage 51 tadpoles after 21 days of exposure. Even though these are numerical stages, they are categorical, defined by distinctive morphological characteristics. The number of tadpoles that were at these stages was reported in Table 3 of the study of Fort et al. (2010), and chi-square analysis was performed. The authors stated that ‘‘Overall, the frequency of each developmental stage in each of the treatment concentrations following 21 days of exposure to TCS was not significantly different from each other (chi-square, p 1⁄4 0.319).’’ It was not clear how the analysis was done, but using the same data as presented in Table 3 in the study of Fort et al. (2010) in a 9 3 5 contingency table with NF 54–56, 63–64, and 65–66 stages grouped and all other stage categories kept separate gave a chi-square 1⁄4 48.7, p 1⁄4 0.029, degrees of freedom 1⁄4 32. In addition, a pairwise 7 3 2 contingency table comparison of the control with each of the TCS treatments individually gave the results shown in Table 1. Therefore, the data presented by Fort et al. (2010) do indeed show that TCS at the three lower exposure concentrations had an effect on the postembryonic development of X. laevis tadpoles. This is consistent with our observations with the bullfrog (Veldhoen et al., 2006.). Moreover, the authors observed that tails from stagematched NF 60 tadpoles showed significantly elevated thyroid hormone receptor b (TRb) transcript levels in the two middle concentrations examined. The 1.5-fold increase observed relative to the control animals was interpreted as ‘‘likely not biologically significant,’’ and the authors point to a lack of concentration dependence of the TCS exposure on altered transcript abundance. Putting this information in context, TRb transcript levels increase in the tadpole tail by approximately sevenfold between NF stages 54 and 60 (Wagner and Helbing,

Collaboration


Dive into the Caren C. Helbing's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Inanc Birol

University of British Columbia

View shared research outputs
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge