Carol J. Lovatt

Plant Growth Regulators in Citriculture: World Current Uses

ABSTRACT Plant growth regulators (PGRs), both naturally occurring and synthetic, are used extensively in citriculture not only to help growers solve production problems, but also to increase the market value of the crop and to improve grower returns. Yield increases are obtained via an improvement in fruit set, particularly for shybearing cultivars, and/or fruit size, especially for the “small-fruit” group of mandarins. Increased fruit number per tree is achieved indirectly through an increase in flower number as a result of better flower initiation or by a direct effect on fruit set. Improved fruit size is brought about directly by stimulating the growth of fruit tissues or indirectly by reducing fruit number by partial inhibition of flower initiation or by subsequent fruit removal. PGRs are also used to hasten maturity, to delay harvest, and to maintain fruit quality postharvest, resulting in greater profits to the grower. This article provides an overview of current uses of PGRs in citriculture. The re...

Plant Growth Regulators in Citriculture: Factors Regulating Endogenous Levels in Citrus Tissues

Abstract Since the review on endogenous growth substances of citrus tissues by Goldschmidt in 1976 (HortScience, 11: 95-99), much information regarding this topic has been published in a wide array of journals. The present review provides a comprehensive overview of published information on endogenous levels of the five classes of plant growth substances (i.e., auxins, cytokinins, gibberellins, ethylene, and abscisic acid), plus polyamines and other endogenous substances that appear to have a role in regulating citrus growth and development. It is the first in a three-part series that next examines hormonal regulation of physiological processes in citrus followed by review of current uses and commercial applications of plant growth regulators in citrus production. In this article, a brief history of the detection and characterization of each class of plant growth substances is given. Following this, variation in endogenous levels associated with different organs (and/or tissues), stages of development, sp...

Effect of harvest date on the nutritional quality and antioxidant capacity in ‘Hass’ avocado during storage

The effect of harvest date on nutritional compounds and antioxidant activity (AOC) in avocado (Persea americana Mill. cv Hass) fruit during storage was determined. The fruits were harvested at seven different dates and ripened at 25 °C following 21 or 35 days of cold storage. The results indicated that the phenolic and glutathione contents were increased and the ascorbic acid content was not significantly different in early harvested fruit (January to March), and the phenolic, ascorbic acid and glutathione contents were increased slightly and then decreased on late harvested fruit (April to June). Similar trends were observed in the changes of AOC. Furthermore, AOC in early harvested fruit after storage for 35 days was much higher than that in late harvested fruit after storage for 21 days. Therefore, avocado can be harvested earlier for economic benefits according to the market and can keep high nutritional value for human health benefits.

Alfalfa (Medicago sativa) carbamoylphosphate synthetase gene structure records the deep lineage of plants.

Given the central role of carbamoylphosphate synthetases in pyrimidine and arginine metabolism in all living organisms, the absence of fundamental information regarding plant CPSase genes is a striking omission [Lawson et al., Mol. Biol. Evol. 13 (1996) 970-977; van den Hoff et al., J. Mol. Evol. 41 (1995) 813-832]. Whereas CPSase gene architecture and aa sequence have proven to be useful characters in establishing ancient and modern genetic affinities, phylogenetic analysis cannot be completed without the inclusion of plant CPSases. We describe the first isolation by molecular cloning of a plant CPSase gene (CPAII) derived from alfalfa (Medicago sativa). DNA sequence analysis reveals a proteobacterial architecture, namely closely linked carA and carB coding domains separated by a short intergenic region, and transcribed as a polycistronic mRNA. CPAII encodes the amino acid residues that typify a CPSase type II enzyme. In addition, an ancient internal duplication has been retained in the plant carB sequence. Partial nucleotide sequencing of additional clones reveals that the alfalfa genome contains multiple CPSase II gene copies which may be tissue-specific in their expression. It appears that with respect to CPSase genes, CPAII resembles the carAB gene of bacteria, and may have preserved much of this ancient gene structure in the alfalfa genome.

Relationship of polyamines to low-temperature stress-induced flowering of the ‘Washington’ navel orange (Citrus sinensis L. Osbeck)

SummaryThe objectives of the present study were to quantify the relationship between flowering and leaf polyamine content at the initiation of, and during, a low-temperature floral-induction treatment and to test the ability of canopy sprays of L-arginine (50 mM), putrescine (10 and 20 mM), and spermidine (10 and 20 mM) to enhance the flowering response of the ‘Washington’ navel orange (Citrus sinensis L. Osbeck). Five year old container-grown ‘Washington’ navel orange trees were subjected to four weeks of low-temperature treatment of 8 h days at PFD of 500 µmol m−2 s−1 and 10°C and 16 h nights at 7°C. Leaf NH3–NH4+, putrescine and spermidine concentrations were significantly greater at the end of, or one day after, the low temperature floral-induction treatment, while spermine content decreased. Trees receiving putrescine (20 mM) or spermidine (10 mM) had significantly greater leaf concentrations of spermidine at the initiation of the low-temperature floral-induction treatment. Flower number per tree was...

Regulation of Purine Metabolism in Intact Leaves of Coffea arabica

The capacity of Coffea arabica leaves (5- x 5-mm pieces) to synthesize de novo and catabolize purine nucleotides to provide precursors for caffeine (1,3,7-trimethylxanthine) was investigated. Consistent with de novo synthesis, glycine, bicarbonate, and formate were incorporated into the purine ring of inosine 5[prime]-monophosphate (IMP) and adenine nucleotides ([sigma]Ade); azaserine, a known inhibitor of purine de novo synthesis, inhibited incorporation. Activity of the de novo pathway in C. arabica per g fresh weight of leaf tissue during a 3-h incubation period was 8 [plus or minus] 4 nmol of formate incorporated into IMP, 61 [plus or minus] 7 nmol into [sigma]Ade, and 150 nmol into caffeine (the latter during a 7-h incubation). Coffee leaves exhibited classical purine catabolism. Radiolabeled formate, inosine, adenosine, and adenine were incorporated into hypoxanthine and xanthine, which were catabolized to allantoin and urea. Urease activity was demonstrated. Per g fresh weight, coffee leaf squares incorporated 90 [plus or minus] 22 nmol of xanthine into caffeine in 7 h but degraded 102 [plus or minus] 1 nmol of xanthine to allantoin in 3 h. Feedback control of de novo purine biosynthesis was contrasted in C. arabica and Cucurbita pepo, a species that does not synthesize purine alkaloids. End-product inhibition was demonstrated to occur in both species but at different enzyme reactions.

Regulation of CPSase, ACTase, and OCTase genes in Medicago truncatula: Implications for carbamoylphosphate synthesis and allocation to pyrimidine and arginine de novo Biosynthesis☆

In most prokaryotes and many eukaryotes, synthesis of carbamoylphosphate (CP) by carbamoylphosphate synthetase (CPSase; E.C. 6.3.5.5) and its allocation to either pyrimidine or arginine biosynthesis are highly controlled processes. Regulation at the transcriptional level occurs at either CPSase genes or the downstream genes encoding aspartate carbamoyltransferase (E.C. 2.1.3.2) or ornithine carbamoyltransferase (E.C. 2.1.3.3). Given the importance of pyrimidine and arginine biosynthesis, our lack of basic knowledge regarding genetic regulation of these processes in plants is a striking omission. Transcripts encoding two CPSase small subunits (MtCPSs1 and MtCPSs2), a single CPSase large subunit (MtCPSl), ACTase (MtPyrB), and OCTase (MtArgF) were characterized in the model legume Medicago truncatula. Quantitative real-time PCR data provided evidence (i) that the accumulation of all CPSase gene transcripts, as well as the MtPyrB transcript, was dramatically reduced following seedling incubation with uridine; (ii) exogenously supplied arginine down regulated only MtArgF; and (iii) mRNA levels of both CPSase small subunits, MtPyrB, and MtArgF were significantly increased after supplying plants with ornithine alone or in combination with uridine or arginine compared to plants treated with only uridine or arginine, respectively (P< or =0.05). A proposed novel, yet simple regulatory scheme employed by M. truncatula more closely resembles a prokaryotic control strategy than those used by other eukaryotes.

Biosynthesis of pyrimidine nucleotides in cultured root callus ofCucurbita pepo

SummaryCallus cultures derived from roots of summer squash (Cucurbita pepo L. c.v. Early Prolific Straightneck) grown in the dark at 27° C on Murashige and Skoog medium supplemented per liter with 30 g sucrose, 100 mg myo-inositol, 10 mg indole-butyric acid, 2 mg glycine, 1 mg thiamin, 0.5 mg nicotinic acid, 0.5 mg pyridoxine, and 2 g Gelrite were capable of synthesizing pyrimidine nucleotides both de novo and through salvage of existing pyrimidine nucleotides and bases. Evidence that the de novo biosynthesis of pyrimidine nucleotides proceeded via the orotate pathway in this tissue included: (a) demonstration of the incorporation of NaH14CO3 and [14C6]orotic acid into uridine nucleotides (ΣUMP), and (b) demonstration that the addition of 6-azauridine blocked the incorporation of these two precursors into ΣUMP.The synthesis of pyrimidine nucleotides through the salvage of existing pyrimidine bases and ribosides was demonstrated by measuring the incorporation of [14C2]uracil and [14C2]uridine into ΣUMP. Salvage of both [14C2]uracil and [14C2]uridine was sensitive to inhibition by 6-azauridine or one of its metabolites.The orotic acid pathway for the de novo biosynthesis of pyrimidine nucleotides was demonstrated to be sensitive to end-product inhibition. Uridine, or one of its metabolites, inhibited the incorporation of NaH14CO3, but not [14C6]orotic acid, into ΣUMP. Evidence is presented suggesting that Aspartate carbomoyltransferase is the site of feedback control.

Application of commerical enzymes to measure the activity of the arginine pathway-urea cycle in intact cells☆

The activity of the complete arginine pathway-urea cycle was assessed in intact plant cells by employing the commercial enzymes arginase (EC 3.5.3.1) and urease (EC 3.5.1.5) to determine the amount of NaH14CO3 incorporated into [guanido-14C]arginine and/or into [14C]urea during a 3-h labeling period. Recovery of [guanido-14C]arginine was linear from 5 to 1000 nmol/g tissue and averaged 80 +/- 5% (mean +/- SE, N = 3). The procedure is reliable, inexpensive, well suited to the simultaneous analysis of numerous samples, and significantly more sensitive than existing methods. The method is ideally suited for assessing the activity of the complete arginine biosynthetic pathway in intact cells. In addition, the method has the distinct advantage of providing simultaneous measurement of the amount of NaH14CO3 accumulating in arginine relative to the amount accumulating as urea. Evidence is presented demonstrating that both the activity of the arginine pathway and the relative amounts of [guanido-14C]arginine and [14C]urea synthesized from NaH14CO3 were influenced by changes in the level of ornithine, NH+4, or phosphorus available to plant tissues.

Gibberellin-Induced Gene Expression Associated with Cytoplasmic Male Sterility in Sunflower

ABSTRACT Gibberellins (GAs) are plant hormones with diverse roles in plant growth and development. Male sterility is one of several GA responses. In an effort to understand the involvements of GAs in sunflower male reproductive development, physiological, biochemical and molecular studies of GA-induced and cytoplasmic male sterility were performed. Male sterility was induced by exogenous application of gibberellic acid (GA3) to the fertile inflorescence apex in early reproductive development. Using different experimental approaches: hormonal quantitative analyses (gas-liquid chromatography), protein profiles (SDS PAGE) and transcripts assays (RT-PCR) revealed a similarity in expression of cytoplasmic (CMS) and induced (IMS) male sterility. Our results provide the first evidence that expression of CMS-specific orfH522 gene can be induced by GA3 in plant with original fertile cytoplasm.