Catherine Tremblay

Potential of Sphagnum peat for improving soil organic matter, water holding capacity, bulk density and potato yield in a sandy soil

Low soil organic matter content and limited soil water holding are the major natural constraint of dryland cropping on sandy soils in the Quebec boreal regions. We conducted a 3-yr (1994–1996) study in a boreal sandy soil, Ferro-Humic Podzol (Spodosols), to determine the potential of Sphagnum peat for improving soil organic matter (SOM), water holding capacity, bulk density (BD), plant leaf nutrient status, and potato and barley yields. The cropping was a rotation of 2-yr potato (Solanum tuberosum L. ‘Superior’) and 1-yr barley (Hordeum vulgare L. ‘Chapais’). The treatments consisted of Sphagnum peat at rates of 0, 29, 48, and 68 Mg ha−1 3-yr−1 on a dry weight basis, and granular N-P-K fertilizers (12-7.5-7) at rates of 1.4, 1.6, and 1.8 Mg ha−1 yr−1, respectively, arranged in a split-block design. The peat-amended soils were higher in water content (SWC), SOM and total porosity but lower in BD and N than neighboring non-peat soils (P < 0.05). Effects of peat and fertilizer treatments and their interaction were significant on potato leaf N, Ca, Mg, and P, tuber yield, dry weight, harvested N and tuber specific gravity (P < 0.05), depending on year. Potato tuber yield and N increased simultaneously up to 30% (compared to the control), and were significantly correlated with SWC, SOM, BD, and NO3-N (−0.52 ≤r ≤ 0.80). In the 3rd year, the linear effect of peat treatments was significant on barley grain yield. In 1995 there was a decline of 4.5−7.3% of SOM of the previous year level. It is suggested that Sphagnum peat at a rate of 48 Mg ha−1 had the potential for improving sandy soil productivity. A longer-term investigation of soil water, N, SOM pool and crop yield changes is necessary to better understand the physical, chemical and biological processes of peat in cropping systems and to maximize the benefits of peat applications.

Efficiency of soil and fertilizer nitrogen of a sod-potato system in the humid, acid and cool environment

It was hypothesized that soil N variability, and fertilization and cropping management affect potato (Solanum tuberosum L.) growth and fertilizer N efficiency. Following a 20-year sod breakup on a loamy soil in eastern Quebec, Canada (46°37′ N, 71°47′ W), we conducted a 3-year (1993–1995) study to investigate the effects of soil pool N and fertilizer N management on non-irrigated potato (cv. Superior) tuber yield, fertilizer N recovery (NRE), and residual N distribution in soils under humid, cool and acid pedoclimatic conditions. The fertilizer N treatments consisted of a control, side-dress at rates of 70, 105 and 140 kg ha−1, and split applications (at seeding and bloom) at rates of 70+70, 105+70 and 140+70 kg ha−1, respectively. Soil acidity was corrected with limestone following the plow down of the sod. Years of cropping, main effect of N treatment, and year and fertilizer N interaction were significant on total and marketable tuber yields and N uptake, which were significantly related to soil N, and root growth. Apparent NRE ranged between 29 and 70%, depending on years and N rates. Total tuber yield, N uptake, soil N use and NRE were significantly higher in the first (sod–potato) year, but decreased by 41.8, 22.7, 21.4 and 14.7%, respectively, in the third (sod–potato–potato–potato) year. Initial soil N pool was declined by 75% following the 3-year cropping. In 2–3 years, the side-dress N (140 kg ha−1) increased significantly tuber yields (11.4–19.8%) compared to the split N (70+70 kg ha−1). Higher split N had no effect on tuber yield and N uptake but increased residual N at harvest. Unused fertilizer N was strongly linked (R2=0.98) to fertilizer N rates. Time factor and N treatment had significant effects (P<0.0001) on loss of N to below the root zone. Smaller scale rate and timing of split N need to be further determined. Increasing fertilizer N use efficiency could be expected with sod breakup and 75% of regional recommendation rate under humid, cool and acid pedoclimatic conditions.

Environmental Mehlich-III soil phosphorus saturation indices for Quebec acid to near neutral mineral soils varying in texture and genesis

The Mehlich-III method (M-III) (Mehlich 1984) is a multinutrient agri-environmental routine soil-testing procedure used in many jurisdictions in North America, but one that is affected by soil texture. The PW determined by the Sissingh (1971) method is an index of surface water contamination and desorbed P that is not influenced by soil texture and that can be used to define specific M-III critical environmental indices by soil texture group. Our objective was to define critical environmental indices by relating (P/Al)M-III to PW. We analyzed 275 soil samples from surface, and 175 from subsurface layers, varying in genesis, texture, and pH. The relationship between PW and (P/Al)M-III was influenced by soil properties, particularly soil texture and genesis. Fine-textured (> 300 g clay kg-1) and gleyed soils tended to release more PW at a given (P/Al)M-III compared with coarse-textured (≤ 300 g clay kg-1) and podzolized soils. Using a critical value of 9.7 mg PW L-1 derived from the literature, critical env...

Mechanical properties of endothelialized fibroblast-derived vascular scaffolds stimulated in a bioreactor

There is an ongoing clinical need for tissue-engineered small-diameter (<6mm) vascular grafts since clinical applications are restricted by the limited availability of autologous living grafts or the lack of suitability of synthetic grafts. The present study uses our self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold that can then be available off-the-shelf. Briefly, scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and then decellularized by immersion in deionized water. Constructs were then endothelialized and perfused for 1week in an appropriate bioreactor. Mechanical testing results showed that the decellularization process did not influence the resistance of the tissue and an increase in ultimate tensile strength was observed following the perfusion of the construct in the bioreactor. These fibroblast-derived vascular scaffolds could be stored and later used to deliver readily implantable grafts within 4weeks including an autologous endothelial cell isolation and seeding process. This technology could greatly accelerate the clinical availability of tissue-engineered blood vessels.

Comparison of the direct burst pressure and the ring tensile test methods for mechanical characterization of tissue-engineered vascular substitutes

Tissue engineering provides a promising alternative for small diameter vascular grafts, especially with the self-assembly method. It is crucial that these grafts possess mechanical properties that allow them to withstand physiological flow and pressure without being damaged. Therefore, an accurate assessment of their mechanical properties, especially the burst pressure, is essential prior to clinical release. In this study, the burst pressure of self-assembled tissue-engineered vascular substitutes was first measured by the direct method, which consists in pressurizing the construct with fluid until tissue failure. It was then compared to the burst pressure estimated by Laplace׳s law using data from a ring tensile test. The major advantage of this last method is that it requires a significantly smaller tissue sample. However, it has been reported as overestimating the burst pressure compared to a direct measurement. In the present report, it was found that an accurate estimation of the burst pressure may be obtained from a ring tensile test when failure internal diameter is used as the diameter parameter in Laplace׳s law. Overestimation occurs with the method previously reported, i.e. when the unloaded internal diameter is used for calculations. The estimation of other mechanical properties was also investigated. It was demonstrated that data from a ring tensile test provide an accurate estimate of the failure strain and the stiffness of the constructs when compared to measurements with the direct method.

A new construction technique for tissue-engineered heart valves using the self-assembly method.

Tissue engineering appears as a promising option to create new heart valve substitutes able to overcome the serious drawbacks encountered with mechanical substitutes or tissue valves. The objective of this article is to present the construction method of a new entirely biological stentless aortic valve using the self-assembly method and also a first assessment of its behavior in a bioreactor when exposed to a pulsatile flow. A thick tissue was created by stacking several fibroblast sheets produced with the self-assembly technique. Different sets of custom-made templates were designed to confer to the thick tissue a three-dimensional (3D) shape similar to that of a native aortic valve. The construction of the valve was divided in two sequential steps. The first step was the installation of the thick tissue in a flat preshaping template followed by a 4-week maturation period. The second step was the actual cylindrical 3D forming of the valve. The microscopic tissue structure was assessed using histological cross sections stained with Massons Trichrome and Picrosirius Red. The thick tissue remained uniformly populated with cells throughout the construction steps and the dense extracellular matrix presented corrugated fibers of collagen. This first prototype of tissue-engineered heart valve was installed in a bioreactor to assess its capacity to sustain a light pulsatile flow at a frequency of 0.5 Hz. Under the light pulsed flow, it was observed that the leaflets opened and closed according to the flow variations. This study demonstrates that the self-assembly method is a viable option for the construction of complex 3D shapes, such as heart valves, with an entirely biological material.

Agri-environmental models using Mehlich-III soil phospho rus saturation index for corn in Quebec

Soil phosphorus (P), which is potentially a risk for environmental contamination, is currently interpreted using soil P saturation in North America. Our objective was to assess the ratio of P to aluminum (Al) in the Mehlich-III (M-III) soil test to build P requirement models for corn and soybean. We analyzed 129 corn and 19 soybean P fertilizer trials. For corn, the (P/Al)M-III ratio improved soil fertility classification compared with PM-III alone. The critical PM-III value as determined by the Cate-Nelson procedure was found to be 31.5 mg PM-III kg-1, close to published values. The critical (P/Al)M-III ratios of 0.025 for > 300 g clay kg-1 soils and 0.040 for ≤ 300 g clay kg-1 soils differed significantly between the two soil groups. For (P/Al)M-III ratios above 0.214, there was no positive response to added P for all soils regardless of texture. Using published critical environmental (P/Al)M-III ratios of 0.076 for > 300 g clay kg-1soils and 0.131 for ≤ 300 g clay kg-1 soils as benchmarks values, agri-...

In Vivo Remodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

There is a clinical need for tissue-engineered small-diameter (<6 mm) vascular grafts since clinical applications are halted by the limited suitability of autologous or synthetic grafts. This study uses the self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold (FDVS) that can be available off-the-shelf. Briefly, extracellular matrix scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and decellularized by immersion in deionized water. The FDVSs were implanted as an aortic interpositional graft in six Sprague-Dawley rats for 6 months. Five out of the six implants were still patent 6 months after the surgery. Histological analysis showed the infiltration of cells on both abluminal and luminal sides, and immunofluorescence analysis suggested the formation of neomedia comprised of smooth muscle cells and lined underneath with an endothelium. Furthermore, to verify the feasibility of producing tissue-engineered blood vessels of clinically relevant length and diameter, scaffolds with a 4.6 mm inner diameter and 17 cm in length were fabricated with success and stored for an extended period of time, while maintaining suitable properties following the storage period. This novel demonstration of the potential of the FDVS could accelerate the clinical availability of tissue-engineered blood vessels and warrants further preclinical studies.

Microstructured human fibroblast-derived extracellular matrix scaffold for vascular media fabrication.

In the clinical and pharmacological fields, there is a need for the production of tissue‐engineered small‐diameter blood vessels. We have demonstrated previously that the extracellular matrix (ECM) produced by fibroblasts can be used as a scaffold to support three‐dimensional (3D) growth of another cell type. Thus, a resistant tissue‐engineered vascular media can be produced when such scaffolds are used to culture smooth muscle cells (SMCs). The present study was designed to develop an anisotropic fibroblastic ECM sheet that could replicate the physiological architecture of blood vessels after being assembled into a small diameter vascular conduit. Anisotropic ECM scaffolds were produced using human dermal fibroblasts, grown on a microfabricated substrate with a specific topography, which led to cell alignment and unidirectional ECM assembly. Following their devitalization, the scaffolds were seeded with SMCs. These cells elongated and migrated in a single direction, following a specific angle relative to the direction of the aligned fibroblastic ECM. Their resultant ECM stained for collagen I and III and elastin, and the cells expressed SMC differentiation markers. Seven days after SMCs seeding, the sheets were rolled around a mandrel to form a tissue‐engineered vascular media. The resulting anisotropic ECM and cell alignment induced an increase in the mechanical strength and vascular reactivity in the circumferential direction as compared to unaligned constructs. Copyright