Chang No Yoon
Korea Institute of Science and Technology
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Chang No Yoon.
PLOS ONE | 2012
Jun-Seok Lee; Yun Kyung Kim; Han Jo Kim; Siti Hajar; Yee Ling Tan; Nam-Young Kang; Shin Hui Ng; Chang No Yoon; Young-Tae Chang
Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCIs 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluorescence intensity analysis, we successfully discriminated the organ origin of all the 60 cell-lines.
Scientific Reports | 2015
Young-Hwa Yoo; JiHyeon Yun; Chang No Yoon; Jun-Seok Lee
Hepatitis C virus (HCV) infection is the leading cause of chronic liver disease that currently affects at least 170 million people worldwide. Although significant efforts have been focused on discovering inhibitors of a viral polymerase (NS5B) or protease (NS3), strategies to cure HCV infection have been hampered by the limited therapeutic target proteins. Thus, discovery of a novel target remains a major challenge. Here, we report a method that combines transcriptome expression analysis with unbiased proteome reactivity profiling to identify novel host cell response factors in HCV infection. A chemical probe for non-directed proteomic profiling was selected based on genome-wide transcriptome expression analysis after HCV infection, which revealed noticeable alterations related to disulfide bond metabolism. On the basis of this result, we screened the proteome reactivity using chemical probes containing thiol-reactive functional groups and discovered a unique labeling profile in HCV-infected cells. A subsequent quantitative chemical proteomic mapping study led to the identification of a target protein, T-plastin (PLST), and its regulation of HCV replication. Our approach demonstrates both a straightforward strategy for selecting chemical probes to discriminate disease states using a model system and its application for proteome reactivity profiling for novel biomarker discovery.
Journal of Biochemistry and Molecular Biology | 2014
Jun-Seok Lee; Young-Hwa Yoo; Chang No Yoon
The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of smallmolecule probes in proteomics studies and highlight some recent examples in which this principle has been applied. [BMB Reports 2014; 47(3): 149-157]
Molecular BioSystems | 2011
Yun Kyung Kim; Jin Kak Lee; Jun-Seok Lee; Chang No Yoon; Young-Tae Chang
A mitochondria-trackable fluorophore, CDy2, selectively labels Cys302 in the active site of mitochondrial aldehyde dehydrogenase (ALDH2).
Chemical Communications | 2015
Dhiraj P. Murale; Seong Cheol Hong; JiHyeon Yun; Chang No Yoon; Jun-Seok Lee
Chemistry & Biology | 2015
Ha-Na Na; Young-Hwa Yoo; Chang No Yoon; Jun-Seok Lee
Chemical Communications | 2014
Jun-Seok Lee; Young-Hwa Yoo; Jihye Kang; Won Seok Han; Jin Kak Lee; Chang No Yoon
Bulletin of The Korean Chemical Society | 2012
Won Seok Han; Jin Kak Lee; Jun-Seok Lee; Hoh-Gyu Hahn; Chang No Yoon
Bulletin of The Korean Chemical Society | 2017
Jin Kak Lee; Won Seok Han; Jun-Seok Lee; Chang No Yoon
Bulletin of The Korean Chemical Society | 2017
Jin Kak Lee; Won Seok Han; Jun-Seok Lee; Yong-Sam Kim; Jeong-Heon Ko; Jong Shin Yoo; Chang No Yoon