Dhiraj P. Murale

Highly Selective Excited State Intramolecular Proton Transfer (ESIPT)-Based Superoxide Probing

Two novel fluorescent probe conjugates of 2-(benzothiazol-2-yl)-phenol (HBT) enable ratiometric and selective superoxide detection by an established excited state intramolecular proton transfer (ESIPT) mechanism giving a 60-fold intensity increase.

Novel and selective detection of Tabun mimics

Detection of nerve agent-related molecules based on BODIPY-salicylaldehyde oxime conjugation was studied. Fluorescence intensity of the B-SAL-OXIME species increases in the presence of DECP, whereas it decreases in the presence of DCP and DEMP (limit of detection = 997 nM). Benzonitrile formation in the novel fluorescent B-SAL-OXIME system was elucidated using model substrates.

Rapid and selective detection of Cys in living neuronal cells utilizing a novel fluorescein with chloropropionate–ester functionalities

A chloropropionate-caged fluorescein probe allows for prompt detection of cysteine over other biothiols, e.g., homocysteine with a limit of detection of 12.8 μM.

Extremely selective fluorescence detection of cysteine or superoxide with aliphatic ester hydrolysis

A novel fluorescence probe modality demonstrated with fluorescein affords a highly selective aqueous-based detection of cysteine over other biothiols, e.g. homocysteine, with a limit of detection of 11.3 μM.