Changhong Huo

Chemical Constituents of the Plants from Genus Jatropha

by Xi-Ping Zhanga), Man-Li Zhanga), Xiao-Huo Sua), Chang-Hong Huoa), Yu-Cheng Gub), and Qing-Wen Shi*a) a) Department of Natural Product Chemistry, School of Pharmaceutical Sciences, Hebei Medical University, 361 Zhongshan East Road, 050017, Shijiazhuang, Hebei Province, P. R. China (e-mail: shiqingwen@hebmu.edu.cn) b) Syngenta Jealott s Hill International Reasearch Centre, Bracknell, Berkshire RG426EY, UK

Chemical constituents of plants from the genus Illicium.

by Yun-Ning Liua), Xiao-Hui Sua), Chang-Hong Huoa), Xi-Ping Zhanga), Qing-Wen Shi*a), and Yu-Cheng Gub) a) Laboratory of Natural Product Chemistry, School of Pharmaceutical Sciences, Hebei Medical University, Shijiazhuang 050017, Hebei Province, P. R. China (phone: þ86-311-86265634; e-mail: shiqingwen@hebmu.edu.cn) b) Syngenta Jealott s Hill International Reasearch Centre, Bracknell, Berkshire RG426EY, UK

Chemical constituents of plants from the genus Xylocarpus.

by Li-Ru Shena), Dong Guob), Yue-Mei Yua), Bao-Wei Yina), Lei Zhaoa), Qing-Wen Shia), Yong-Li Wanga), and Chang-Hong Huo*a) a) School of Pharmaceutical Sciences, Hebei Medical University, 361 Zhongshan East Road, 050017 Shijiazhuang, P. R. China (phone: þ8631186265634, e-mail: rainbowhuo@hebmu.edu.cn) b) North China Pharmaceutical Group Corporation, New Drug R&D Co., Ltd., Shijiazhuang, P. R. China

Chemical constituents of the plants of the genus Calophyllum.

by Xiao-Hui Sua), Man-Li Zhanga), Li-Geng Lia), Chang-Hong Huoa), Yu-Cheng Gub), and Qing-Wen Shi*a) a) Department of Natural Product Chemistry, School of Pharmaceutical Sciences, Hebei Medical University, 361 Zhongshan East Road, Shijiazhuang 050017, Hebei Province, P. R. China (phone: þ86-311-8626-5634; e-mail: shiqingwen@hebmu.edu.cn) b) Syngenta Jealott s Hill International Research Centre, Bracknell, UK-Berkshire RG426EY

Achillinin A, a Cytotoxic Guaianolide from the Flower of Yarrow, Achillea millefolium

Achillinin A (2β,3β-epoxy-1α,4β,10α-trihydroxyguai-11(13)-en-12,6α-olide, 1), a new guaianolide isolated from the flower of Achillea millefolium, exhibited potential antiproliferative activity to A549, RERF-LC-kj and QG-90 cells with 50% inhibitory concentration (IC50) values of 5.8, 10 and 0.31 μM, respectively.

Two new eudesmanes from Inula helenium

Two new eudesmanes, 1α-hydroxy-11,13-dihydroisoalantolactone (1), 3α-hydroxy-11,13dihydroalantolactone (2), and one known compound were isolated from the roots of Inula helenium. Their structures were established on the basis of spectral evidence.

Polyhydroxytriterpenoids and Phenolic Constituents from Forsythia suspensa (Thunb.) Vahl Leaves

Forsythia suspensa (Thunb.) Vahl leaves have been consumed in China as a health-promoting functional tea for centuries. Three new polyhydroxytriterpenoid glycosides named suspensanosides A-C (1-3), seven known polyhydroxytriterpenoids (4-10), and 12 known phenolics (11-22) were identified from F. suspensa leaves. Compounds 1-10, 15-17, and 22 have not been found in the Forsythia genus previously, whereas compound 14 was first reported to be isolated from the leaves of F. suspensa. All isolates were tested for their antiproliferative activities on BGC-823 and MCF-7 human tumor cell lines, whereas all phenolics were further investigated for their antioxidant activities by a DPPH assay. The results clearly demonstrate that triterpenoids, especially ursane-type triterpenoids, and the diverse phenolic components are crucial for the beneficial effects of F. suspensa leaves.

Xylomexicanins C and D, New Mexicanolide-Type Limonoids from Xylocarpus granatum

Two new limonoids, named xylomexicanins C and D, were isolated from a dichloromethane extract of the seeds of Xylocarpus granatum cultivated in Hainan, China, and their structures were elucidated on the basis of one- and two-dimensional NMR (including 1H, 13C-NMR, DEPT, 1H-1H COSY, HSQC, HMBC, and NOESY) and confirmed by high-resolution mass spectrometry. Xylomexicanin C exhibited antiproliferative activity against human breast carcinoma cells (KT).

Taxanes from the leaves of Taxus cuspidata.

A new taxane, 13-oxobaccatin III (2), and five known taxanes, 10-deacetyl-13-oxobaccatin III (1), baccatin III (3), 7-epi-10-deacetyltaxol (4), 19-debenzoyl-19-acetyltaxinine M (5), and 5a-decinnamoyltaxagifine (6), were isolated from the leaves of the Japanese yew, Taxus cuspidate. Their structures were established on the basis of the analysis of their spectral data.

Identification of in vitro and in vivo metabolites of alantolactone by UPLC-TOF-MS/MS

Alantolactone (AL), an active sesquiterpene originating from Inula helenium, is a potential anticancer and anti-inflammatory agent. However so far, studies on AL metabolism have not been reported. In the present study, we have investigated for the first time the in vivo and in vitro metabolites of AL using ultra performance liquid chromatography combined with time of flight mass spectrometry (UPLC-TOF-MS/MS). A unique on-line information-dependent acquisition (IDA) method multiple mass defect filter (MMDF) combined with dynamic background subtraction (DBS) was applied to trace all of the probable metabolites of AL. Five MMDF templates were set according to the core structure of AL and the general metabolite biotransformation patterns, and other five sulfur-containing dimer filter templates were first established on the basis of structural elucidation of AL metabolites obtained from rat intestinal bacteria biotransformation. As a result, 44 metabolites were characterized: 41 metabolites from rat urine, bile and feces after oral administration of AL, and 13 metabolites from AL biotransformation by rat intestinal bacteria. Particularly, 26 metabolites were identified as novel sulfur-containing products. The results indicated that addition of double bond at Δ((11,13)) and oxidization were the main metabolic reactions of AL. A new metabolism pathway to produce addition products of H2S to AL and further generate a series of sulfur-containing dimers of AL was revealed. This study significantly enriched our knowledge about AL metabolism, which will lead to a better understanding of the safety and efficacy of AL. At the same time, the established methodology can be widely applied for the structural determination of the metabolites of other sesquiterpene containing α-methylene-γ-lactone moiety.