Chantal Marion

Composition and antimalarial activity in vitro of volatile components of lippia multiflora

The essential oil of Lippia multiflora was prepared by hydrodistillation of leaves and stalks and characterized by GC and mass spectroscopy. The oil was tested for antimalarial activity on in vitro cultures of Plasmodium falciparum (FcB1-Columbia chloroquine-resistant strain and F32-Tanzania chloroquine-sensitive strain). The dilutions inhibiting the in vitro growth of the parasite by 50% 24 and 72 hr after administration of the essential oil to the parasite culture were 1/12,000 and 1/21,000, respectively. When tested on a highly synchronized culture, the essential oil inhibited growth mostly at the trophozoite-schizont step, indicating a potential effect on the first nuclear division of the parasite.

Volatile Components of Annona muricata L.

ABSTRACT GC/MS analysis of the leaf, peel and fruit pulp oils of Annona muricata L. showed the presence of 68 compounds of which 59 were identified. The main components of the leaf oil were β-caryophyllene (31.4%) and other sesquiterpenes, while the fruit oil contained essentially aliphatic acids and esters, in particular, methyl (E)-2-hexenoate (39.8%).

A Chemical, Bacteriological, Toxicological and Clinical Study of the Essential Oil of Lippia multiflora Mold. (Verbenaceae)

ABSTRACT The leaf oil of Lippia multiflora was examined by GC and GC/MS and found to contain (Z)-tagetone (11.3%) and (E)-tagetone (30.2%) as major constituents. It was found to be highly active against isolated microorangisms of the buccal flora. A mouthwash prepared at a dilution of 1/500 of the essential oil showed the same activity as the oil. Tests for toxicity proved negative. The results obtained thus far reveal a particularly promising potential for the large scale use of L. multiflora.

Volatile Composition of Fourteen Species of Fresh Wild Mushrooms (Boletales)

Abstract Fourty-five volatile substances of fourteen species of fresh wild mushrooms (Boletus aereus, Boletus calopus, Chroogomphus rutilus, Gomphidius glutinosus, Leccinum aurantiacum, Leccinum lepidum, Leccinum pulchrum, Leccinum querci-num, Leccinum versipelle, Paxillus atrotomentosus, Suillus bovinus, Suillus collinitus, Suillus granulatus, Suillus variegatus) have been identified by GC/MS. The main components of the fruit bodies were 1-octen-3-ol, (E)-2-octenol, 1-octen-3-one, octanol, 3-octanone, 3-octanol, N(2-phenylethyl)acetamide, benzaldehyde, limonene, geranyl acetone, farnesyl acetone and (E,E)-farnesol. Camphene and germacrene D were identified for the first time in mushrooms.

In vitro Cytotoxic Activity of Three Essential Oils from Salvia Species

Abstract Essential oils of Salvia officinalis L., S. sclarea L. and S. lavandulifolia Vahl. (Lamiaceae) prepared by hydrodistillation were characterized by GC/MS. The oils were found to possess the following major components: S. officinalis: 1,8-cineole (11.7%), α-thujone (65.5%) and β-thujone (15.4%); S. sclarea: linalool (10.7%) and linalyl acetate (81.1%) and S. lavandulifolia: 1,8-cineole (25.5%), camphor (39.0%) and linalyl acetate (10.2%). These oils were tested for cytotoxic activities on cultures of different human tumor cell lines. Cytotoxic activity was compared to that of the anticancer agent doxorubicin. The oils of S. officinalis and S. lavandulifolia were 100 to 1000 times less active than doxorubicin on all the cell lines. S. sclarea oil showed a strong in vitro activity against cell suspensions in the same range as doxorubicin and was 100 times less active on attached cell cultures.

Investigation of Some Volatile Components of Seven Fresh Wild Mushrooms (Basidiomycetes)

Twenty-five volatile compounds were identified by GC/MS from seven fresh wild mushrooms (Piptoporus betulinus, Oligoporus caesius, Amanita rubescens, Paxillus involutus, Suillus grevillei, Suillus luteus, Xerocomus subtomentosus—Basidiomycetes). The main volatile components were 1-octen-3-ol, 3-heptanone, limonene, (E)-2-heptenal, (E)-2-decenal, 4-hydroxy-4-methyl-2-pentanone, methyl 2,4-dihydroxy-3,6-dimethylbenzoate, farnesyl acetone, 3-octanol and 3-octanone. Geranyl acetone and 1,8-cineole were identified for the first time in mushrooms.

Caffeic glycoside esters from Jasminum nudiflorum and some related species

Poliumoside, forsythoside B, echinacoside and arenarioside, caffeic glycoside esters, were isolated from several species of Oleaceae. The poliumoside/forsythoside ratio distinguishes Jasminum nudiflorum from J. mesnyi. Arenarioside and forsythoside B act in Forsythia species as good hybridization markers.

Volatile Components of Annona squamosa L.

ABSTRACT GC/MS analysis of the essential oil and extract of the leaves and die fruits of Annona squamosa L. showed the presence of 107 compounds of which 70 were identified. The main components of the leaf oil, accounting for 82% of the oil, were germacrene D (17.5%), β-elemene (12%), α- and β-pinene (8.1%), sabinene (8.8%), bicyclogermacrene (6%), T-cadinol (5.5%) and T-muurolol (4.4%), while the fruit oil contained spathulenol (17.8–37%), bornyl acetate (1.8–7.1%), germacrene D (0.5–9.6%), borneol (0.2–3.7%) and verbenone (0.5–4.5%).

Volatile Components of Stachys Corsica Pers. (Lamiaceae)

Abstract Nineteen volatile components were identified by GC/MS from the dichlo-romethane extract of air-dried Stachys corsica. Carvacryl acetate, linalool, α-terpenyl acetate and α-terpineol were the main volatiles.

Volatile Constituents of Fruit Pulp of Dialium guineense Wild. (Cesalpinaceae)

Abstract A concentrate of the hydrodistilled volatiles from the pulp of fruits of Dialium guinense Wild, were analyzed by GC and GC/MS. The analysis showed the presence of alkanes (38%), acids (26.6%), aldehydes (13.5%) and alcohols (12.1%).