Chaohui Gao

Determination of methanol and ethanol synchronously in ternary mixture by NIRS and PLS regression

This paper reports the usefulness of partial least squares (PLS) in the analysis of NIR spectra. Based on this work, the ethanol and methanol could be measured synchronously in the chemical industry. It is demonstrated that the proposed technique is quite convenient and efficient. PLS regression has been applied to establish a satisfactory calibration model via adopting optimum wavelength. In this model, the correlation coefficient in methanol determination is 0.99991 and the root mean square error of calibrate (RMSEC) is 0.431; in ethanol determination, the correlation coefficient is up to 0.99998, and the RMSEC is 0.193. Compared with the measured value of GC in the sample determination, the range of relative error is from 0.721% to 3.505%.

Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Method

According to the amino acid sequence and codon preference of E. coli, the human interleukin-18(IL-18) gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.