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Dive into the research topics where Charles R. Reagan is active.

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Featured researches published by Charles R. Reagan.


Journal of Clinical Investigation | 1973

Metabolic Effects of Plasmin Digests of Human Growth Hormone in the Rat and Man

John B. Mills; Charles R. Reagan; Daniel Rudman; Jack L. Kostyo; P. Zachariah; Alfred E. Wilhelmi

As a first step in our study of structure-function relationships among primate and non-primate growth hormones, human growth hormone (hGH) was subjected to the limited digestive activity of human plasmin. The lyophilized whole digest, containing less than 2% of unchanged hormone, had an average of 2.3 new amino-terminal groups per mole. The digest had the same potency as the native hormone (a) in causing weight gain in hypophysectomized rats; (b) in stimulating somatomedin production in hypophysectomized rats; (c) in stimulating upake of [(3)H]leucine into isolated diaphragm of hypophysectomized rats; (d) in accelerating transport of [(14)C]alpha-aminoisobutyric acid into isolated diaphragm of hypophysectomized rats; (e) in stimulating uptake of [3-0-methyl-(14)C]glucose by isolated adipose tissue of hypophysectomized rats; (f) in accelerating conversion of [(14)C]glucose to (14)CO(2) by isolated epididymal adipose tissue of hypophysectomized rats. The digest also caused glucosuria in partially pancreatectomized rats treated with dexamethasone. These metabolic actions of plasmin-digested hGH in the array of animal tests were confirmed by comparable effects elicited in 11 human subjects (nine pituitary-deficient children and adolescents and two nondeficient adults). A single injection of the plasmin digest caused an increase in plasma free fatty acids and a fall in plasma amino acids. Seven daily injections caused positive balances of nitrogen, phosphorous, sodium, and potassium, gain in body weight, and in two of three subjects impairment of glucose tolerance. The potency of the plasmin digest in producing these metabolic effects in man was comparable to that of native hGH.Thus, 2-3 bonds in the hGH molecule can be cleaved by plasmin without impairing the hormones growthpromoting, anabolic, diabetogenic, and adipokinetic actions for rat and man.


Endocrinology | 1980

Specific Binding of Growth Hormone by Rat Adipocytes

Katherine D. Fagin; Suzette Lackey; Charles R. Reagan; Mario DiGirolamo


The Journal of Clinical Endocrinology and Metabolism | 1974

Effects of Human Somatomedin Preparations on Membrane Transport and Protein Synthesis in the Isolated Rat Diaphragm

Knut Uthne; Charles R. Reagan; Lynn P. Gimpel; Jack L. Kostyo


Endocrinology | 1980

Fragments of Human Growth Hormone Produced by Digestion with Thrombin: Chemistry and Biological Properties

John B. Mills; Jack L. Kostyo; Charles R. Reagan; S. A. Wagner; Martha H. Moseley; Alfred E. Wilhelmi


Endocrinology | 1978

Hourly Variations in Plasma Concentrations of Growth Hormone and Insulin and in Amino Acid Uptake and Incorporation into Protein in Diaphragm Muscle of the Rat

Olle Isaksson; David F. Nutting; Jack L. Kostyo; Charles R. Reagan


Endocrinology | 1975

Isolation and Biological Characterization of Fragments of Human Growth Hormone Produced by Digestion with Plasmin

Charles R. Reagan; John B. Mills; Jack L. Kostyo; Alfred E. Wilhelmi


Endocrinology | 1978

Isolation and Characterization of Fragments of Reduced and S-Carbamidomethylated Human Growth Hormone Produced by Plasmin Digestion. II. Biological and Immunological ActivitiesEndocrinology 102:*

Charles R. Reagan; Jack L. Kostyo; John B. Mills; Martha H. Moseley; Alfred E. Wilhelmi


Pharmacology & Therapeutics. Part B: General and Systematic Pharmacology | 1976

The biology of growth hormone

Jack L. Kostyo; Charles R. Reagan


Endocrinology | 1981

Recombination of Fragments of Human Growth Hormone: Altered Activity Profile of the Recombinant Molecule*

Charles R. Reagan; Jack L. Kostyo; John B. Mills; S. E. Gennick; J. L. Messina; S. A. Wagner; Alfred E. Wilhelmi


Endocrinology | 1979

Use of Antibodies to Modify the Effect of Growth Hormone on Sugar Transport in Rat Diaphragm Muscle

Olle Isaksson; Jessica Schwartz; Jack L. Kostyo; Charles R. Reagan

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