Cheryl Capehart

Photoperiod, early post-natal eye growth, and visual deprivation

(1) We studied the influence of photoperiod and unilateral lid suture on post-natal ocular growth in two types of White Leghorn chicks previously reported to respond differently to visual deprivation, Truslow and Cornell K chicks. We analyzed the chicks after 2 weeks of rearing, a time period commonly used in neuropharmacological studies of eye growth but much shorter than in most prior studies of photoperiod effects on the chick eye. (2) Altering the photoperiod length significantly influenced the refraction and growth of both open and sutured eyes even at this early time, with differences between the two types of chicks. (3) The most prominent effect on the open eyes was the development of hyperopia with rearing under constant light, a response especially prominent in the Cornell K chicks. In the open eyes under this condition, the anterior chamber shallowed and the vitreous chamber elongated in the axial dimension only, reciprocal changes that resulted in no net alteration of axial length at 2 weeks. A high variability in refraction of open eyes reared with constant illumination suggests the need for a dark period in the regulation of eye growth. (4) Compared to contralateral open eyes, the lid-sutured eyes of both types of chicks developed longer total axial lengths and enlarged vitreous chambers in both axial and equatorial dimensions under each photoperiod. The effects on anterior chamber depth and refraction were complex and differed between the two types of chicks. (5) The responses in open eyes support the notion that growth of the vitrious chamber of the chick eye is differentially regulated in the axial and equatorial dimensions, previously indicated by pharmacological studies. The responses in both open and sutured eyes indicate different control mechanisms for anterior chamber and vitreous cavity growth.

Local Patterns of Image Degradation Differentially Affect Refraction and Eye Shape in Chick

Purpose: To evaluate visual blur as a mechanism for modulating eye shape. Methods: Chicks wore a unilateral full goggle or one of several goggles modified with apertures. After 2 weeks, eyes were measured with refractometry, ultrasound, and calipers, and three retinal regions were assayed for dopamine and DOPAC (3,4-dihydroxyphenylacetic acid). Results: Goggled eyes were diffusely enlarged or enlarged predominantly along the axial dimension, depending on the goggle. Myopia developed under goggle types inducing primarily axial growth and under some of the goggles inducing diffuse eye expansion. Enlarged eyes remained emmetropic beneath other goggles that caused diffuse eye expansion. Reductions in retinal dopamine and DOPAC were proportional to the eye growth and refraction effects. Conclusions: Localized image degradation can cause myopia with predominantly axial expansion, myopia with more diffuse vitreous chamber expansion, or eye expansion without myopia. Robust expansion of the equatorial diameter alone was not observed. The associated alterations in retinal dopamine metabolism are consistent with a hypothesized role of dopaminergic amacrine cells in the visual regulation of eye growth. Besides refraction and overall size, visual blur can affect eye shape; but the goggle responses do not correspond to a simple summation of blur signals across the retina. Therefore, other mechanisms seemingly are needed to account for the full range of refractions and ocular shapes seen in chicks and, by analogy, in humans.

The ciliary ganglion and vitreous cavity shape

PURPOSE To learn the influence of the ciliary ganglion on the postnatal growth of eyes with unimpaired visual input and of eyes beneath an image diffusing goggle. METHODS Newborn chicks received unilateral ciliary ganglionectomy or unilateral sham operation and were reared either with or without a goggle ipsilateral to the surgical procedure. Ocular refractions and ultrasound measurements were made on anesthetized chicks; eyes enucleated postmortem were measured in axial and equatorial dimensions with calipers and studied histologically. RESULTS Excessive growth of open eyes in the equatorial dimensions followed ciliary ganglionectomy and became more pronounced as the chicks grew older. There was only a modest increase in axial growth. Ganglionectomy also induced relative hyperopia; lens thinning contributed to this effect and likely was a direct result of disrupted parasympathetic input to the ciliary muscle. Ganglionectomy also slightly increased the thickness of the choroid in the posterior pole but not in more peripheral locations. CONCLUSION We conclude that the ciliary ganglion exerts an inhibitory influence on the postnatal growth of open eyes; the main effect is in the equatorial dimension of the vitreous cavity, with a smaller effect on axial length. Ciliary ganglionectomy exerted minimal influence on the development of experimental myopia, known to be induced by the goggle regimen. The amount of equatorial expansion in goggle-induced myopia was greater than after ganglionectomy alone, indicating that other factors besides the ciliary ganglion can influence the equatorial dimension of the vitreous cavity.

Corneal surface area: an index of anterior segment growth.

Corneal surface area and perimeter were assessed as novel indices to monitor anterior segment growth, using chicks reared under different photoperiods. We obtained central and mid‐peripheral corneal curvatures using photokeratometry. Anatomical tracings of the anterior corneal surface also were made from freeze‐dried non‐fixed preparations of the anterior segments of the same eyes. Using either photokeratometry or anatomical data, the profile of the anterior corneal surface was fit to a general equation for conical sections; corneal surface area was estimated from surfaces of revolution. Optical techniques modeled the chick cornea as a circle or as an ellipse closely resembling a circle. The anatomical technique, in contrast, modeled the chick corneal profile as a hyperbola. Potential explanations of this discrepancy are discussed. Regardless of which model is evaluated, the corneal surface area and perimeter of two‐week‐old chicks are affected by the photoperiod of rearing. Corneal surface area in particular proved more sensitive than conventional measurements in identifying anterior segment effects of rearing under different photoperiods. Analysis of corneal area may prove useful in evaluating the mechanisms governing anterior segment growth.

Anterior Segment Growth and Peripheral Neural Pathways in Chick

Purpose: To learn if peripheral nerve pathways are necessary for corneal expansion and anterior segment growth under a 12-hr light:dark cycle or for the inhibition of corneal expansion under constant light rearing. Methods: Recently hatched White Leghorn chicks under anesthesia received unilateral ciliary ganglionectomy (CGx), cranial cervical ganglionectomy (Sx), or section of the ophthalmic nerve (TGx), along with sham-operated and/or never-operated control cohorts. Chicks were reared postoperatively under either a 12-hr light:dark cycle or under constant light. After 2 weeks and with the chicks under anesthesia, corneal radii of curvature and diameters were obtained with a photokeratoscope, refractometry and A-scan ultrasonography were performed, and the axial and equatorial dimensions of enucleated eyes were measured with digital calipers. Corneal areas were calculated from corneal curvatures and diameters. Results: Despite the rich peripheral innervation to the eye, the selective denervations performed here exerted remarkably limited effects on corneal expansion and anterior segment development in chicks reared under either lighting condition. Ophthalmic nerve section did reverse in large part the inhibition of equatorial expansion of the vitreous chamber occurring under constant light rearing. Conclusions: The ciliary, sympathetic, or ophthalmic peripheral nerve pathways to the eye are not required either for corneal expansion and anterior segment development under a 12-hr light:dark cycle or for the inhibition of corneal expansion under constant light rearing. The ocular sensory innervation may be a means for regulating vitreous cavity shape.

Distinctive Mechanisms Regulating Corneal and Scleral Growth

To learn if the cornea adjusts passively to vitreous cavity expansion or if its growth is subject to an independent regulatory mechanism, chicks were raised under photoperiods with 12 or 24 hours of light. As previously described, continuous light rearing caused vitreous cavity enlargement, anterior chamber shallowing and corneal flattening compared to the other photoperiods. We previously found that, when assessed by optical or anatomical methods, the chick corneal surface area at 2 weeks of age was larger following rearing under a 12 hour light:dark photoperiod than under continuous light. With a spherical model for the chick corneal shape, optical methods suggest that corneal growth slows rather than arrests under continuous light, when vitreous cavity expansion is greatest. The photoperiod-related dissociation of vitreous cavity and corneal growth indicates that scleral and corneal expansion can be differentially regulated. Because of photoperiod dependency, neural mechanisms likely modulate post-natal corneal expansion.